Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice

Zeng, Zhiwen; Huang, Jinquan; Chen, Yong; Xiao, Yu; Zhu, Wei; Zhang, Dong-Shu

doi:10.1007/s11655-022-3579-7

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…Additionally, T-cell response was modified from pathogenic Th2 to protective Th1. 12 Furthermore, IFN-γ + CD4 + T cells were also found to be elevated in the AWB group. These changes in Treg and Th1 cells were accompanied by alterations in systemic cytokine production in both the AWB and AD-IgG groups, with increased mRNA levels of IL-10 and IFN-γ in the dorsal skin tissue.…”

Section: Discussionmentioning

confidence: 92%

“… 33 There is speculation that injections of autologous whole blood or total IgG into specific acupoints may have an immunomodulatory effect in AD mice by manipulating the dose and type of antigen-presenting cells that come into contact with the antigen. 12 , 30 In this way, autologous blood injections act as personalized vaccines, stimulating the immune system to recognize and respond to AD components, increasing neutralizing modulators, inducing tolerance, and restoring homeostasis. 34 The potential mechanism of A-AHT may be activation of Treg cells by anti-idiotypic immunomodulation because acupoint injections of autologous whole blood, autologous total IgG, or heterologous total IgG improved the clinical severity of AD in AD mice.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that A-AHT can regulate Th1/Th2 immune responses, which is a key factor in AD progression, through the continuous stimulation of acupoints and blood components, and the selectivity of acupoints (ST 36 and LI 11) and injection (AWB compared to normal saline). 12 …”

Section: Introductionmentioning

confidence: 99%

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Acupoint Autohemotherapy Attenuates DNCB-Induced Atopic Dermatitis and Activates Regulatory T Cells in BALB/c Mice

Yan,

Chen,

Huang

et al. 2024

JIR

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Acupoint autohemotherapy (A-AHT) has been proposed as an alternative and complementary treatment for atopic dermatitis (AD), yet the exact role of its blood component in terms of therapeutic efficacy and mechanism of action is still largely unknown. Methods: This study aimed to evaluate the therapeutic efficacies and action mechanisms of intramuscular injections of autologous whole blood (AWB) and mouse immunoglobulin G (IgG) (autologous or heterologous) at acupoints on 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse models. Serum levels of total immunoglobulin E (IgE), IgG, interleukin-10 (IL-10), and interferongamma (IFN-γ) were measured, as well as mRNA expression levels of Forkhead box P3 (FoxP3), IL-10 and IFN-γ in dorsal skin lesions, and IL-10 + , IFN-γ + and FoxP3 + CD4 + T cells in murine spleen. Results: It showed that repeated acupoint injection of AWB, autologous total IgG (purified from autologous blood in AD mice) or heterologous total IgG (purified from healthy blood in normal mice) effectively reduced the severity of AD symptoms and decreased epidermal and dermal thickness as well as mast cells in skin lesions. Additionally, AWB acupoint injection was found to upregulate FoxP3 + , IL-10 + and IFN-γ + CD4 + T cells in murine spleen, suppressing the production of IgE antibodies and increasing that of IgG antibodies in the serum. Furthermore, both AWB and autologous total IgG administrations significantly elevated FoxP3 expression, mRNA levels of IL-10 and IFN-γ in dorsal skin lesions. However, acupoint injection of heterologous total IgG had no effect on regulatory T (Treg) and Th1 cells modulation. Conclusion: These findings suggest that the therapeutic effects of A-AHT on AD are mediated by IgG-induced activation of Treg cells.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Acupoint Autohemotherapy Attenuates DNCB-Induced Atopic Dermatitis and Activates Regulatory T Cells in BALB/c Mice

Yan,

Chen,

Huang

et al. 2024

JIR

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“…Previous studies have discovered that peripheral inflammation is related to the peripheral T lymphocytes-secreted cytokines [ 12 , 13 ]. Th1 and Th2 are the two important subsets of Th cells, Th1 cells mainly secrete the pro-inflammatory cytokines to participate in macrophage activation for mediating cellular immunity [ 14 , 15 ], such as interleukin-6 (IL-6) [ 16 ], Interferon-γ (IFN-γ) [ 17 ]. By contrast, Th2 cells mainly secrete inhibitory cytokines like IL-4 and IL-10 [ 18 , 19 ] to mediate humoral immunity.…”

Section: Discussionmentioning

confidence: 99%

PCSK6 mediates Th1 differentiation and promotes chronic colitis progression and mucosal barrier injury via STAT1

Mei

Zhou

Song

et al. 2023

Aging

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This study was aimed at investigating the expression and role of proprotein convertase subtilisin/kexin type (PCSK6) in inflammatory bowel disease (IBD). DSS induced mouse colitis and mucosal barrier injury, down-regulation of TJ proteins, improvement of permeability, and increases of the proportions of Th1 and M1 macrophages. After PCSK6 knockdown, the colitis in KO mice was improved relative to WT mice, the TJ protein levels increased, and the proportions of Th1 and M1 macrophages decreased. STAT1 inhibitor treatment also inhibited chronic colitis in mice. As revealed by in-vitro experiments, PCSK6 overexpression promoted the transformation of Th0 into Th1, while PCSK6 silencing suppressed the transfection. COPI assay results revealed the presence of targeted binding relation between PCSK6 and STAT1. PCSK6 binds to STAT1 to promote STAT1 phosphorylation and regulate Th1 cell differentiation, thus promoting the M1 polarization of macrophages and aggravating colitis progression. PCSK6 is promising as the new target for the treatment of colitis.

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“…A comprehensive scoring system (Additional file 1 : Table S1) adapted by Scoring Atopic Dermatitis (SCORAD) [ 27 ] was developed to measure the severity of skin symptoms by scoring erythema, edema, scales, lichenification, ear thickness and scratch frequency [ 28 ] with 0 (none), 1 (mild), 2 (moderate) and 3 (severe) [ 29 , 30 , 31 , 32 ]. The scratch frequency was measured an hour after DNCB application with duration of ten minutes, and multiple scratches within one second were regarded as one event.…”

Section: Methodsmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cell treatment alleviates symptoms in an atopic dermatitis-like mouse model

et al. 2023

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Background Atopic dermatitis (AD) is one of the most common immune and inflammatory skin disorders, leading to insufferable itching and skin abnormalities that seriously affect life quality of patients. There are still huge unmet needs for long-term and effective disease control, despite currently available therapies. Evidenced by some preclinical and clinical studies of AD treatment with stem cells, stem cell treatment could significantly and effectively ameliorate AD symptoms. Objectives To elucidate underlying mechanisms of how stem cells therapy alleviates AD-like symptoms. Methods An AD-like mouse model was constructed and treated with mesenchymal stem cells (MSCs) subcutaneously or subcutaneously combined with intravenously. The differentially expressed genes were sorted out from RNA sequencing results of dorsal skin and blood. Results Two injection routes of MSCs could alleviate AD-like symptoms and pathologic changes of the skin and immune organs. RNA sequencing of dorsal skin sections and blood provided gene expression signatures for amelioration of skin defects, inflammatory and immune modulation by MSCs, as well as common AD molecular markers for the skin and blood, which may benefit for clinical diagnosis. IL-1β and its signaling pathway were specifically found to be associated with the development of AD-like dermatitis lesions. MSC treatment effectively inhibited the JAK-STAT pathway and receptors of IL-4, IL-13, IL-17, and IgE. Conclusions MSC therapy could regulate abnormal immune and inflammatory status in AD. Mechanistic exploration will contribute to the development of personalized AD treatment based on MSCs.

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Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice

Cited by 10 publications

References 23 publications

Acupoint Autohemotherapy Attenuates DNCB-Induced Atopic Dermatitis and Activates Regulatory T Cells in BALB/c Mice

Acupoint Autohemotherapy Attenuates DNCB-Induced Atopic Dermatitis and Activates Regulatory T Cells in BALB/c Mice

PCSK6 mediates Th1 differentiation and promotes chronic colitis progression and mucosal barrier injury via STAT1

Human umbilical cord mesenchymal stem cell treatment alleviates symptoms in an atopic dermatitis-like mouse model

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