Acts of Attention: The Poems of D. H. Lawrence

Spector, Robert D.; Lawrence, D. H.; Gilbert, Sandra M.

doi:10.2307/40128577

Cited by 3 publications

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“…Murine CMV establishes acute, persistent, and latent infections in the mouse (reviewed in references 13, 14, 29, and 30). The map position of a limited number of genes with homology between human and murine CMV suggests that these two viruses may have a colinear genome arrangement (3,18,19,24,26,43).…”

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Cytomegalovirus determinant of replication in salivary glands

Manning

Stoddart

Lagenaur

et al. 1992

J Virol

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Murine cytomegalovirus carrying a deletion mutation disrupting the expression of a gene dispensable for growth in cultured cells was found to disseminate poorly in the mouse. The mutation resulted in a dramatic decrease in the expression of a 1.5-kb major and a 1.8-kb minor 1a transcript from a region adjacent to the ie2 gene in the viral genome. Nucleotide sequence determination indicated that 323 bp, including a predicted polyadenylation signal, was deleted from this 1a gene. In cultured cells, the plaque morphology and growth characteristics of the mutant were similar to those of parental or rescued wild-type viruses. Following intraperitoneal inoculation of BALB/c mice, growth of the mutant in the salivary gland was dramatically reduced 10,000-fold, while growth in the liver and spleen was not dramatically affected. The 13 gene was thus denoted sggl (salivary gland growth gene 1). Neither intranasal infection nor direct inoculation into the salivary glands completely overcame the restriction of growth in this organ, suggesting that the sggl gene encoded a determinant of tissue tropism. To investigate the impact of the sggl mutation on virus dissemination via the blood, the virus titer in peripheral blood leukocytes was determined. No difference was found between the sggl mutant and rescued wild-type virus. Thus, murine cytomegalovirus sggl gene products appear to be involved in entry or replication of virus in salivary gland cells.

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confidence: 99%

Cytomegalovirus determinant of replication in salivary glands

Manning

Stoddart

Lagenaur

et al. 1992

J Virol

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“…Foot and mouth disease virus (FMDV), like end, we sought an alternative procedure for its poliovirus, is a member of the family Picorna-location. viridae. Both of these viruses contain a single-Spector and Baltimore (25) showed that the stranded RNA molecule that acts directly as poly(A) tract of poliovirus RNA could be re-mRNA and contains a tract of polyadenylic acid moved by digestion with Escherichia coli [poly(A)] at the 3' end (1,5,18,26). The 5' end RNase H in the presence of oligodeoxythymiof picornavirus RNA is not capped (9,15,20); dylic acid [oligo(dT)].…”

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confidence: 99%

More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA

Rowlands¹,

Harris²,

Brown³

1978

J Virol

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The polycytidylic acid [poly(C)] tract in foot and mouth disease virus RNA has been located about 400 nucleotides from the 5' end of the RNA by analysis of the products from the digestion of the RNA with RNase H in the presence of oligodeoxyguanylic acid [oligo(dG)]. This treatment produces a small fragment (S) containing the small protein covalently linked to the RNA and a large fragment (L) that migrates faster than untreated RNA on low-percentage polyacrylamide gels, lacks the poly(C) tract as shown by RNase T1 digestion and oligo(dG)-cellulose binding, and is no longer infective. Polyacrylamide gel electrophoresis of fragment S suggests that it is about 400 nucleotides long, in agreement with the size estimated from the proportion of radioactivity in the fragment.Analysis of the RNase T, digestion products of S shows that it contains only those oligonucleotides mapping close to the poly(C) tract that is situated near the 5' end of the virus RNA.Foot and mouth disease virus (FMDV), like end, we sought an alternative procedure for its poliovirus, is a member of the family Picorna-location.viridae. Both of these viruses contain a single-Spector and Baltimore (25) showed that the stranded RNA molecule that acts directly as poly(A) tract of poliovirus RNA could be re-mRNA and contains a tract of polyadenylic acid moved by digestion with Escherichia coli [poly(A)] at the 3' end (1,5, 18, 26). The 5' end RNase H in the presence of oligodeoxythymiof picornavirus RNA is not capped (9, 15, 20); dylic acid [oligo(dT)]. This enzyme is an endohowever, poliovirus RNA has recently been nuclease that specifically hydrolyzes the RNA shown to have a small protein covalently linked component of DNA:RNA hybrids (2). In a simto the 5'-terminal nucleotide, and FMDV RNA ilar approach, the specific removal of the poly(C) also contains a covalently linked protein (8, 17, tract from FMDV RNA by digestion with RNase 19, 24).H in the presence of oligodeoxyguanylic acid In addition to poly(A), the RNAs of enceph-[oligo(dG)] to obtain a 5' fragment is described alomyocarditis virus (EMCV), mengovirus, and in this paper. The size of the 5' end fragment FMDV contain a tract of polycytidylic acid gives a measure of the distance of the poly(C) [poly(C)] 100 to 200 nucleotides long (3, 22, 23). tract from the 5' end of the RNA. This sequence has been located near the 5' end of EMCV RNA by showing that only full-length MATEPIALS AND METHODS RNA bound to both polyuridylic acid-and po-

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“…It has been speculated that poly(A) plays a role in transport and/or processing of viral precursor RNA into mature cytoplasmic messen ger or genomic RNA (93). Removal of poly(A) from polio viral RNA results in concurrent loss of RNA infectivity (139,140). Similar experi ments with oncoviral or virus-specific mRNA would be interesting.…”

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confidence: 97%

The Gene Order of Avian RNA Tumor Viruses Derived from Biochemical Analyses of Deletion Mutants and Viral Recombinants

Lh¹

1978

Annu. Rev. Microbiol.

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Acts of Attention: The Poems of D. H. Lawrence

Cited by 3 publications

References 0 publications

Cytomegalovirus determinant of replication in salivary glands

Cytomegalovirus determinant of replication in salivary glands

More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA

The Gene Order of Avian RNA Tumor Viruses Derived from Biochemical Analyses of Deletion Mutants and Viral Recombinants

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