Actomyosin II contractility expels von Willebrand factor from Weibel–Palade bodies during exocytosis

Nightingale, Thomas D.; White, Ian J.; Doyle, Emily L.; Turmaine, Mark; Harrison‐Lavoie, Kimberly J.; Webb, Kathleen F.; Cramer, Louise P.; Cutler, Daniel F.

doi:10.1083/jcb.201011119

Cited by 123 publications

(201 citation statements)

References 58 publications

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“…Furthermore, it was found that to release the content of these vesicles, Myosin2a and 2b recruitment and activity were required to provide the contractile force necessary to complete fusion of the vesicle with the plasma membrane (Masedunskas, et al, 2011). Similar observation has been reported for the secretion of von Willebrand factor from human endothelial cells (Nightingale, et al, 2011).…”

Section: Phospho-regulation Beyond Fusionsupporting

confidence: 60%

Molecular Machinery Regulating Exocytosis

Shandala¹,

Kakavanos-Plew²,

Ng³

et al. 2012

Crosstalk and Integration of Membrane Trafficking Pathways

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Section: Phospho-regulation Beyond Fusionsupporting

confidence: 60%

Molecular Machinery Regulating Exocytosis

Shandala¹,

Kakavanos-Plew²,

Ng³

et al. 2012

Crosstalk and Integration of Membrane Trafficking Pathways

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“…The ability of a cell to achieve a complex, polarized architecture also relies on actomyosin contractility (Lee et al, 2012;Walters et al, 2006;Yu et al, 2008) as does its ability to migrate (Aguilar-Cuenca et al, 2014;Vicente-Manzanares et al, 2009). Moreover, actomyosin contractility regulates several aspects of endosome organization and trafficking (Chandrasekar et al, 2014;Lynch et al, 2013;Masedunskas et al, 2011;Nightingale et al, 2011;Olazabal et al, 2002;Zilberman et al, 2011).…”

Section: Cellular Functions Of the Contractomementioning

confidence: 99%

The contractome – a systems view of actomyosin contractility in non-muscle cells

Zaidel-Bar

Guo

Luxenburg

2015

Journal of Cell Science

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Actomyosin contractility is a highly regulated process that affects many fundamental biological processes in each and every cell in our body. In this Cell Science at a Glance article and the accompanying poster, we mined the literature and databases to map the contractome of non-muscle cells. Actomyosin contractility is involved in at least 49 distinct cellular functions that range from providing cell architecture to signal transduction and nuclear activity. Containing over 100 scaffolding and regulatory proteins, the contractome forms a highly complex network with more than 230 direct interactions between its components, 86 of them involving phosphorylation. Mapping these interactions, we identify the key regulatory pathways involved in the assembly of actomyosin structures and in activating myosin to produce contractile forces within non-muscle cells at the exact time and place necessary for cellular function.

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“…To better understand drug-packaging MP-mediated tumour cell killing, the mechanism involved in tumour cell release and uptake of MPs was investigated. The cytoskeleton has important roles in cellular endocytosis and exocytosis [23][24][25][26] , leading to the possible requirement of cytoskeletal proteins for the release and uptake of MPs by tumour cells. When H22 cells were treated with cytochalasin D, an inhibitor of F-actin polymerization, actin filament formation was inhibited, resulting in decreased MP release, induced by ultraviolet irradiation (Fig.…”

Section: Cisplatin-encapsulating Mps Inhibit Ovarian Cancer Growthmentioning

confidence: 99%

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

et al. 2012

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Cellular microparticles are vesicular plasma membrane fragments with a diameter of 100-1,000 nanometres that are shed by cells in response to various physiological and artificial stimuli. Here we demonstrate that tumour cell-derived microparticles can be used as vectors to deliver chemotherapeutic drugs. We show that tumour cells incubated with chemotherapeutic drugs package these drugs into microparticles, which can be collected and used to effectively kill tumour cells in murine tumour models without typical side effects. We describe several mechanisms involved in this process, including uptake of drug-containing microparticles by tumour cells, synthesis of additional drug-packaging microparticles by these cells that contribute to the cytotoxic effect and the inhibition of drug efflux from tumour cells. This study highlights a novel drug delivery strategy with potential clinical application.

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Actomyosin II contractility expels von Willebrand factor from Weibel–Palade bodies during exocytosis

Abstract: High-resolution microscopy reveals how discrete actin cytoskeletal functions inhibit or promote specific exocytic steps during regulated secretion.

Cited by 123 publications

References 58 publications

Molecular Machinery Regulating Exocytosis

Molecular Machinery Regulating Exocytosis

The contractome – a systems view of actomyosin contractility in non-muscle cells

Delivery of chemotherapeutic drugs in tumour cell-derived microparticles

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