Activity Test of Ethanol Extract from Pulai Bark (Alstonia scholaris L.) Antiinflammation in Mice (Mus musculus)

Abstract

Inflammation is a complex biological response of vascular tissue to noxious stimuli such as pathogens, damaged body cells, or irritants. The drugs commonly used are non-steroidal anti-inflammatory drugs. However, long-term use of non-steroidal anti-inflammatory drugs can cause ulceration and bleeding in the lower GI tract. So that alternative treatments that can be used are medicinal plants, one of which is the wood of pulai. Pulai bark (Alstonia scholaris L.) contains secondary metabolites of alkaloids, flavonoids, polyphenols, terpenoids and steroids. Flavonoids are able to provide anti-inflammatory effects. The aim of this study was to determine the anti-pain activity of the ethanol extract of the bark of the bark of pulai (Alstonia scholaris L.) on the inflammation of mice (Mus musculus). Extraction was carried out by continuous maceration method (remaceration) using 96% ethanol as solvent. Anti-pain testing on inflammation of mice using the true experimental method, mice were divided into 5 groups, namely the negative control group was given aquadest, the positive control was given 0.39 g/30-g BW of diclofenac sodium, the test group was given ethanol extract of pulai bark as much as 0.5 mL/30 g BW with a dose of 400 mg/kgBW, 200 mg/kgBW and 100 mg/kgBW. After 1 min, 1% acetic acid was induced at a dose of 10 mL/30 gBW. Observation of healing time was carried out by observing the stretching of the mice that had been given treatment, which was marked by reduced stretching of the mice. The data were analyzed statistically using the One Way Anova test. The data obtained showed that the ethanol extract of pulai bark at a dose of 400 mg/kgBW, 200 mg/kgBW and 100 mg/kgBW had an anti-pain effect on inflammation in mice. The effective dose as an anti-pain in inflammatory mice is a dose of 200 mg/kgBW.