Activity, substrate detection and immunolocalization of glutathione peroxidase (GPx) in bovine reproductive organs and semen

Vaisberg, C.; Jelezarsky, Lachezar Vasilev; Dishlianova, B; Chaushev, Todor A.

doi:10.1016/j.theriogenology.2004.12.009

Cited by 28 publications

(17 citation statements)

References 16 publications

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“…Quantification of antioxidant enzymes was performed after western blotting. We observed one protein band of 23 kDa and 1 between 50 and 75 kDa corresponding to GPXBI and GPXBS respectively (Vaisberg et al 2005, Drevet 2006; Fig. 3A).…”

Section: Induced Lipid Peroxidation Effects In Ram Spermmentioning

confidence: 88%

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Hamilton¹,

Castro²,

Delgado³

et al. 2016

Reproduction

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Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm.

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Section: Induced Lipid Peroxidation Effects In Ram Spermmentioning

confidence: 88%

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Hamilton¹,

Castro²,

Delgado³

et al. 2016

Reproduction

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show abstract

“…Although catalase is of limited importance in the testes, there are several isoforms of GPx in this tissue that use glutathione (GSH) as a source of electrons to reduce H 2 O 2 to water. It has been reported that in the mitochondria, nucleus, and acrosomal domain of differentiating spermatozoa [35]. The phospholipid hydroperoxide GPx is one of the most important isoforms in a testicular context and is highly expressed in both spermatogenic and Leydig cells [36].…”

Section: Discussionmentioning

confidence: 99%

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Ansar

Abudawood

Hamed

et al. 2016

Biol Trace Elem Res

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Metal nanomaterials hold great potential and play an important role in consumer products. However, the increasing use of nanomaterials has raised concern over inadvertent exposure and potential risks for human health and the environment. Henceforth, in vivo testing of nanoparticles and protection against its toxicity is required. Using rat as an animal model, effect of sodium selenite (Se), an essential trace element, on rat testes exposed to silver nanoparticles (AgNPs) was evaluated. Male rats were treated with AgNPs (5 mg/kg/b.w) i/p or Se (0.2 mg/kg/b.w) by gavage. AgNP administration decreased Glutathione (GSH) levels and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and increased levels of malondialdehyde (MDA) and expression of interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α). However, treatment with Se increased GSH levels and activities of SOD, CAT, and GPx compared with AgNP-treated group and decreased the level of MDA and inflammatory biomarkers significantly (p < 0.05) as compared with AgNP-treated group. Light microscopic analyses also revealed that AgNP induced histopathological changes in testes tissue. Further, protection by Se on biochemical results was confirmed by alleviation of the histopathological changes in the tissue. Results show the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis, and suggest that Se possesses significant potential in reducing AgNP-induced testicular toxicity.

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“…They are concentrated in the mitochondria, nucleus and acrosomal domain of differentiating spermatozoa 14. The phospholipid hydroperoxide GPx (PHGPx) is one of the most important GPx isoforms in a testicular context and is highly expressed in both spermatogenic and Leydig cells 15.…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

Antioxidant Systems and Oxidative Stress in the Testes

Aitken

Roman

2008

Oxidative Medicine and Cellular Longevity

670

471

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Activity, substrate detection and immunolocalization of glutathione peroxidase (GPx) in bovine reproductive organs and semen

Cited by 28 publications

References 16 publications

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Antioxidant Systems and Oxidative Stress in the Testes

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