Activity of the yeast FLP recombinase in Arabidopsis

Sonti, Ramesh V.; Tissier, Alain; Wong, Donny; Viret, Jean-Frédéric; Signer, Ethan R.

doi:10.1007/bf00032673

Cited by 34 publications

(22 citation statements)

References 30 publications

(36 reference statements)

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“…Transgene excision-activation via site-specific recombination in extra-and intra-chromosomal environments has been so far demonstrated in a growing number of plant species including tobacco Ow 1990, 1991;Odell et al 1990;Onouchi et al 1991;Bayley et al 1992;Russell et al 1992;Lloyd and Davis 1994;Kilby et al 1995: Bar et al 1996Gidoni et al 2001b), Arabidopsis (Russell et al 1992;Kilby et al 1995;Onouchi et al 1995;Sonti et al 1995;Luo et al 2000;Hoff et al 2001;Marjanac et al 2008), petunia (Que et al 1998;Coppoolse et al 2003), tomato (Stuurman et al 1996;Coppoolse et al 2003;Zhang et al 2006, Gidoni et al unpublished results), potato (Cuellar et al 2006;Kondrak et al 2006), soybean (Li et al 2007), strawberry (Schaart et al 2004), wheat (Srivastava et al 1999), rice (Endo et al 2002;Hoa et al 2002;Toriyama et al 2003;Radhakrishnan and Srivastava 2005;Sreekala et al 2005;Hu et al 2008), maize (Lyznik et al 1996;Srivastava and Ow 2001;Zhang et al 2003;Kerbach et al 2005), turfgrass (Hu et al 2006), Brassica juncea (Indian mustard; Arumugam et al 2007), citrus (Ballester et al 2007), aspen, and snapdragon . This progress, along with progress made in development of tran...…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 98%

“…However, retransformation is lengthy and cross-fertilization-dependent strategies are applicable only for sexually propagated species with relatively short reproduction cycles. Additionally, constitutive overexpression of recombinase proteins may cause aberrant developmental phenotypes in plants (Sonti et al 1995;Que et al 1998;Coppoolse et al 2003). Therefore, alternative approaches based on transient gene expression have been developed.…”

Section: Introductionmentioning

confidence: 99%

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Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants

Gidoni

Srivastava

Carmi

2008

In Vitro Cell.Dev.Biol.-Plant

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A major limitation of crop biotechnology and breeding is the lack of efficient molecular technologies for precise engineering of target genomic loci. While transformation procedures have become routine for a growing number of plant species, the random introduction of complex transgenenic DNA into the plant genome by current methods generates unpredictable effects on both transgene and homologous native gene expression. The risk of transgene transfer into related plant species and consumers is another concern associated with the conventional transformation technologies. Various approaches to avoid or eliminate undesirable transgenes, most notably selectable marker genes used in plant transformation, have recently been developed. These approaches include cotransformation with two independent T-DNAs or plasmid DNAs followed by their subsequent segregation, transposon-mediated DNA elimination, and most recently, attempts to replace bacterial T-DNA borders and selectable marker genes with functional equivalents of plant origin. The use of site-specific recombination to remove undesired DNA from the plant genome and concomitantly, via excision-mediated DNA rearrangement, switch-activate by choice transgenes of agronomical, food or feed quality traits provides a versatile "transgene maintenance and control" strategy that can significantly contribute to the transfer of transgenic laboratory developments into farming practice. This review focuses on recent reports demonstrating the elimination of undesirable transgenes (essentially selectable marker and recombinase genes) from the plant genome and concomitant activation of a silent transgene (e.g., a reporter gene) mediated by different site-specific recombinases driven by constitutive or chemically, environmentally or developmentally regulated promoters. These reports indicate major progress in excision strategies which extends application of the technology from annual, sexually propagated plants towards perennial, woody and vegetatively propagated plants. Current trends and future prospects for optimization of excision-activation machinery and its practical implementation for the generation of transgenic plants and plant products free of undesired genes are discussed.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants

Gidoni

Srivastava

Carmi

2008

In Vitro Cell.Dev.Biol.-Plant

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“…Some site-specific recombination systems have been shown to function efficiently in heterologous cellular environments. For example, FLP/FRT from the 2 lm plasmid of Saccharomyces cerevisiae (Broach et al 1982), or Cre/lox from E. coli phage P1 (Austin et al 1981) have been shown to catalyze DNA recombination in tobacco (Lioyd and Davis 1994;Qin et al 1994;Bar et al 1996;Bayler and Hess 2005), Arabidopsis (Odell et al 1990;Dale and Ow 1991;Bayler et al 1992;Russell et al 1992;Kilby et al 1995;Osborne et al 1995;Sonti et al 1995;Luo et al 2000), tomato (Stuurman et al 1996), maize and rice (Lyznik et al 1993(Lyznik et al , 1995Hoa et al 2002;Radhakrishnan and Srivastava 2005;Sreekala et al 2005) as well as wheat (Srivastava et al 1999). This provides an excellent tool for controlled modification of plant genomes including chromosomal deletions, inversions, transpositions (Ow 1996;Luo and Kausch 2002) or site-specific gene targeting (Albert et al 1995;Srivastava and Ow 2002).…”

Section: Introductionmentioning

confidence: 95%

FLP-mediated site-specific recombination for genome modification in turfgrass

Nelson

Luo

2006

Biotechnol Lett

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To develop molecular strategies for gene containment in genetically modified (GM) turfgrass, we have studied the feasibility of using the FLP/FRT site-specific DNA recombination system from yeast for controlled genome modification in turfgrass. Suspension cell cultures of creeping bentgrass (Agrostis stolonifera L.) and Kentucky bluegrass (Poa pratensis) were co-transformed with a FLP recombinase expression vector and a recombination-reporter test plasmid containing beta-glucuronidase (gusA) gene which was separated from the maize ubiquitin (ubi) promoter by an FRT-flanked blocking DNA sequence to prevent its transcription. GUS activity was observed in co-transformed cells, in which molecular analyses indicated that FLP-mediated excision of the blocking sequence had brought into proximity the upstream promoter and the downstream reporter gene, resulting in GUS expression. Functional evaluation of the FLP/FRT system using transgenic creeping bentgrass stably expressing FLP recombinase confirmed the observation in suspension cell culture. Our results indicate that FLP/FRT system is a useful tool for genetic manipulation of turfgrass, pointing to the great potential of exploiting the system to develop molecular strategies for transgene containment in perennials.

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“…Wu et al, 2000;Johansson et al, 2003;Hao et al 2005;Filichkin et al, 2006b). Although gene excision has been used in poplar, most notably as part of the MAT system (Ebinuma et al, 1997;Matsunaga et al 2002;Zelasco et al, 2007), a number of other useful and well studied excision systems such as CRE/LOX (Marjanac et al, 2008) and FLP/FRT (Sonti et al, 1995) have not, to our knowledge, ever been characterized for their effectiveness in poplar. Nevertheless, technological advance in other species seem to be transferable to poplar (Fig.…”

Section: Future Prospects and Challengesmentioning

confidence: 99%

Transformation as a Tool for Genetic Analysis in Populus

Busov

Strauss

Pilate³

2009

Genetics and Genomics of Populus

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We summarize the outlook for using transformation as a genetic tool in Populus. Transformation approaches avoid the major obstacle to performing genetics experiments in trees -namely long generation cycles and the difficulty of inbreeding to reveal loss of function alleles. Dominant transgenic alleles allow modifications in gene function to be readily observed in primary transformants. Although transformation has been mainly used for reverse genetics (where the gene sequence of interest is known), transgenic mutagenesis approaches such as activation tagging and gene/enhancer traps have also been shown to enable forward genetics (where the phenotype, not the gene, is known). We outline challenges and needs for more efficient use of transformation tools. These include expansion of the transformation toolbox (e.g., promoters, vectors, targeting), and improved ability to conduct field trials to study gene function in native and plantation environments (in spite of regulatory obstacles). Because of the power of transformation, it will remain a major genetic research tool for dissection of gene function in Populus for many years to come. It is the key biological attribute that makes poplar the most powerful model organism for genetic analysis of woody plant growth, adaptation, and development.

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Activity of the yeast FLP recombinase in Arabidopsis

Cited by 34 publications

References 30 publications

Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants

Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants

FLP-mediated site-specific recombination for genome modification in turfgrass

Transformation as a Tool for Genetic Analysis in Populus

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