Activity of the Germline-Specific &lt;I&gt;Oct4&lt;/I&gt;-GFP Transgene in Normal and Clone Mouse Embryos

Boiani, Michele; Kehler, James; Schöler, Hans R.

doi:10.1385/1-59259-741-6:001

Cited by 22 publications

(19 citation statements)

References 44 publications

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“…Y324C/Y324C (Laird et al, 2011) and Ror2 −/− (Arora et al, 2014) mice were mated with Oct4-ΔPE eGFP (Oct4-GFP) (Boiani et al, 2004) to label germ cells. Busulfan (Sigma, B2635) (Douglas et al, 2013) was injected intraperitoneally (20 mg/ml in 40 μl) in pregnant E11.5 CD1 or FVB females crossed with C57/Bl6;Oct4-GFP males.…”

Section: Ror2mentioning

confidence: 99%

Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary

Arora

Ross

Cantu

et al. 2016

Journal of Cell Science

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Mouse ovarian germ cells enter meiosis in a wave that propagates from anterior to posterior, but little is known about contribution of germ cells to initiation or propagation of meiosis. In a Ror2 mutant with diminished germ cell number and migration, we find that overall timing of meiotic initiation is delayed at the population level. We use chemotherapeutic depletion to exclude a profoundly reduced number of germ cells as a cause for meiotic delay. We rule out sex reversal or failure to specify somatic support cells as contributors to the meiotic phenotype. Instead, we find that anomalies in the distribution of germ cells as well as gonad shape in mutants contribute to aberrant initiation of meiosis. Our analysis supports a model of meiotic initiation via diffusible signal(s), excludes a role for germ cells in commencing the meiotic wave and furnishes the first phenotypic demonstration of the wave of meiotic entry. Finally, our studies underscore the importance of considering germ cell migration defects while studying meiosis to discern secondary effects resulting from positioning versus primary meiotic entry phenotypes.

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Section: Ror2mentioning

confidence: 99%

Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary

Arora

Ross

Cantu

et al. 2016

Journal of Cell Science

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“…Expression is lost during differentiation [219][220][221][222][223]. Reciprocally, OCT3/4 downregulation initiates differentiation [216] or, in PGCs, apoptosis [217,224,225].…”

Section: Oct3/4 and Nanogmentioning

confidence: 99%

An oncofetal and developmental perspective on testicular germ cell cancer

Rijlaarsdam

Looijenga

2014

Seminars in Cancer Biology

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“…Oct3/4 expression starts at the morula stage and becomes restricted at first to the inner cell mass during blastocyst formation, subsequently to the epiblast during gastrulation and finally to primordial germ cells (PGC) (Rosner et al, 1990, Scholer et al, 1989. PGC lacking Oct3/4 expression has been shown to undergo apoptosis rather than differentiation (Boiani et al, 2004).…”

Section: Embryonic Stem/germ Cells and Pluripotencymentioning

confidence: 99%