Activity of terbinafine in experimental fungal infections of laboratory animals

Petrányi, G; Meingassner, Josef G.; Mieth, H.

doi:10.1128/aac.31.10.1558

Cited by 87 publications

(51 citation statements)

References 5 publications

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“…Terbinafine interferes with ergosterol biosynthesis by inhibiting a membrane-bound squalene epoxidase (31). Inhibition of squalene epoxidase results in ergosterol deficiency with the accumulation of squalene, which may be responsible for the observed "in vitro" fungicidal activity (11,26).…”

mentioning

confidence: 99%

Terbinafine Resistance Mediated by Salicylate 1-Monooxygenase inAspergillus nidulans

Graminha

Rocha

Prade

et al. 2004

Antimicrob Agents Chemother

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Resistance to antifungal agents is a recurring and growing problem among patients with systemic fungal infections. UV-induced Aspergillus nidulans mutants resistant to terbinafine have been identified, and we report here the characterization of one such gene. A sib-selected, 6.6-kb genomic DNA fragment encodes a salicylate 1-monooxygenase (salA), and a fatty acid synthase subunit (fasC) confers terbinafine resistance upon transformation of a sensitive strain. Subfragments carrying salA but not fasC confer terbinafine resistance. salA is present as a single-copy gene on chromosome VI and encodes a protein of 473 amino acids that is homologous to salicylate 1-monooxygenase, a well-characterized naphthalene-degrading enzyme in bacteria. salA transcript accumulation analysis showed terbinafine-dependent induction in the wild type and the UV-induced mutant Terb7, as well as overexpression in a strain containing the salA subgenomic DNA fragment, probably due to the multicopy effect caused by the transformation event. Additional naphthalene degradation enzyme-coding genes are present in fungal genomes, suggesting that resistance could follow degradation of the naphthalene ring contained in terbinafine.

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mentioning

confidence: 99%

Terbinafine Resistance Mediated by Salicylate 1-Monooxygenase inAspergillus nidulans

Graminha

Rocha

Prade

et al. 2004

Antimicrob Agents Chemother

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“…There was no intention to clarify whether the ozonized oil has a fungistatic effect; nevertheless, if it does, this data may account for the fact that the clinical improvement of lesions with the oil was slower in comparison with the use of terbinaphine, a well-proven fungicide product (19).…”

Section: Discussionmentioning

confidence: 99%

The use of ozonized oil in the treatment of dermatophitosis caused by Microsporum canis in rabbits

Daud

Ueda

Navarini

et al. 2011

Braz. J. Microbiol.

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The ozone is effective against most microorganisms due to its high oxidant power. Low concentrations and short-term contact are sufficient to inactivate bacteria, mold, yeast, parasites, seaweeds, protozoa and fungi. Microsporum canis is an important agent of dermatophitosis in human and animal. The aim of the current study was to assess the efficacy of ozonized oil over Microsporum canis in rabbits. Eighteen male New Zealand white rabbits, weight ranging from 2 to 3.2 kg were depilated in the cranial dorso-lateral and right caudal, and cranial and left caudal regions. The regions were inoculated with Microsporum canis, excepting the right caudal region, and were denominated TM, O, OM and M, respectively. After seven days, the treatment of lesions in TM began with 0.12g of terbinaphine 1% cream; in OM and O with 0.12g of ozonized oil; all animals were treated once a day for 28 days. Region M was not treated. Material was collected from those regions for cultivation in Sabouraud agar at day 28 of treatment. In the evolution of the treatment with terbinaphine, of 14 contaminated regions with Microsporum canis ten evolved to cure.With the ozonized oil, of 15 contaminations, four were cured. Clinically, that is, the macroscopic evaluation of lesions showed improvement in the TM and OM treated regions. We can conclude that there was statistical evidence of the protection action of the oil against the dermatophyte.

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“…21,22) The inoculum concentration of M. gypseum conidia was 1ϫ10 7 conidia/ml. Before inoculation, the hairs of both flanks of each guinea pig were widely plucked manually, and the hairless skin was slightly abraded with a blade.…”

Section: Antifungal Effect Of Eugenol and Nerolidol Against Microspormentioning

confidence: 99%

“…Clinical assessment of inoculated skin area was performed using a modified lesion score from 0 to 4 as indicated: score 0, no visible lesion; score 1, only hair loss; score 2, well defined redness with few scales; score 3, well defined redness with large areas of scaling; and score 4, ulceration and scarring in addition to a score 3 lesion. 21) Hair samples from inoculated skin were plucked and put onto Sabouraud dextrose agar for fungal growth. Upon identification of a fungal colony, the sample was considered positive.…”

Section: Antifungal Effect Of Eugenol and Nerolidol Against Microspormentioning

confidence: 99%

Antifungal Effect of Eugenol and Nerolidol against Microsporum gypseum in a Guinea Pig Model

Lee

Han

Lee

et al. 2007

Biological & Pharmaceutical Bulletin

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Dermatophytosis is a superficial infection caused by keratinophilic fungi, known as dermatophytes, in keratinized tissues including hair, nail and stratum corneum of skin. The dermatophytes include three genera: Microsporum, Trichophyton and Epidermophyton. The species that most commonly infect animals are Microsporum canis, M. gypseum and Trichopyton mentagrophyte. The fungi invade into keratinized tissues and hair follicles, and cause patchy alopecia, scale, and subsequent inflammation.1,2) Traditional antifungal drugs have variable adverse effects, and fungal resistance is becoming more common. Thus, more potent and safer antifungal agents need to be developed. [3][4][5] Various plant materials are believed to have antifungal activity, and many essential oils have been reported to have antifungal activities. [6][7][8] Japanese cypress (Chamaecyparis obtusa) is an evergreen tree in the Cupressaceae family with antibacterial and anticandidal activities as well as a sedative, stress-reducing effect which decreases serum cortisol levels.9,10) Eugenol and nerolidol are extracted from an essential oil from Japanese cypress. Eugenol is a major phenolic component of clove oil used in dentistry as a root canal sealer. Eugenol has been reported to inhibit the growth of bacteria, including oral bacteria 11) and some gram positive and negative bacteria (Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogens), Lactobacillus sakei) by interaction with the cell membrane.12,13) Anticandidal activities of Eugenol have also been described in vitro and in vivo. 14,15) Silva et al. demonstrated that eugenol, a main constituent of essential oils of Ocimum gratissimum, has antidermatophytic activities in vitro; this effect has not been confirmed in vivo. Nerolidol is a sesquiterpenoid component of essential oil used to enhance flavor and aroma, and has been studied as a topical skin penetration enhancer. [16][17][18] In addition, nerolidol has inhibitory activities on S. aureus and E. coli by altering bacterial cell permeability, 19,20) but it has not been evaluated for antifungal activities.Thus, in the present study, antifungal activities of the essential oils, eugenol and nerolidol, were evaluated against dermatophytes. The antifungal activities of these biomaterials were assessed against one dermatophyte, M. gypseum, using the microdilution method in a guinea pig model. 21,22) MATERIALS AND METHODS Experimental AnimalsIn the present study, 20 male albino guinea pigs (5-week old, 350 to 400 g) were obtained from Dae Han Biolink Co., Ltd. (Eumsung, Chungbuk, Korea). All animals were individually housed in polycarbonate cages, and used after acclimation to an environmentally controlled room (temperature: 23Ϯ2°C, relative humidity: 50Ϯ10%, frequent ventilation and 12 h light cycle). All experimental and animal procedures were approved by the Ethics Committee of the Chungbuk National University.Test Materials Eugenol and nerolidol were extracted from Japanese cypress at the Department...

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Activity of terbinafine in experimental fungal infections of laboratory animals

Cited by 87 publications

References 5 publications

Terbinafine Resistance Mediated by Salicylate 1-Monooxygenase inAspergillus nidulans

Terbinafine Resistance Mediated by Salicylate 1-Monooxygenase inAspergillus nidulans

The use of ozonized oil in the treatment of dermatophitosis caused by Microsporum canis in rabbits

Antifungal Effect of Eugenol and Nerolidol against Microsporum gypseum in a Guinea Pig Model

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