Activity of taurolidine gels on ex vivo periodontal biofilm

Pirracchio, Luca; Joos, A.; Luder, Nina; Sculean, Anton; Eick, Sigrun

doi:10.1007/s00784-017-2297-6

Cited by 15 publications

(13 citation statements)

References 50 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, the total counts of colony forming units (CFU) of the biofilms were counted after 6 h and 24 h incubation in an anaerobic atmosphere at 37 • C. After 24 h of incubation, the quantity and metabolic activity of the biofilms were also measured. The quantification was made by staining with crystal violet according to recently published protocols [25,26]. In short, after washing, the biofilms were fixed at 60 • C for 1 h. Thereafter, 50 µL of 0.06% crystal violet solution was given per well.…”

Section: Activity On Biofilm Formationmentioning

confidence: 99%

“…After 10 min of incubation at room temperature, the stained biofilms were dissolved by 200 µL of 30% acetic acid, and after transferring to a new microtiter plate, the OD 600nm was measured. Biofilm metabolic activity was assessed using resazurin as a redox indicator [25,27]. After short washing of the biofilms, 100 µL of nutrient broth containing 0.06 mM resazurin (Merck KGaA) per well was added.…”

Section: Activity On Biofilm Formationmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

et al. 2020

Self Cite

View full text Add to dashboard Cite

Periodontal therapy using antimicrobials that are topically applied requires slow or controlled release devices. The in vitro antimicrobial activity of biodegradable polymer formulations that contain a new minocycline lipid complex (P-MLC) was evaluated. The new P-MLC formulations that contained 11.5% minocycline were compared with pure minocycline or an existing commercial formulation, which included determination of minimal inhibitory concentration (MIC) values against two oral bacteria and activity on six-species periodontal biofilm. Moreover, the flow of gingival crevicular fluid (GCF) was modeled up to 42 days and the obtained eluates were tested both for MIC values and inhibiting biofilm formation. In general, MICs of the P-MLC formulations were slightly increased as compared with pure minocycline. Biofilm formation was clearly inhibited by all tested formulations containing minocycline with no clear difference between them. In 3.5 day old biofilms, all formulations with 250 µg/mL minocycline decreased bacterial counts by 3 log10 and metabolic activity with no difference to pure antimicrobials. Eluates of experimental formulations showed superiority in antimicrobial activity. Eluates of one experimental formulation (P503-MLC) still inhibited biofilm formation at 28 days, with a reduction by 1.87 log10 colony forming units (CFU) vs. the untreated control. The new experimental formulations can easily be instilled in periodontal pockets and represent alternatives in local antimicrobials, and thus warrant further testing.

show abstract

Section: Activity On Biofilm Formationmentioning

confidence: 99%

Section: Activity On Biofilm Formationmentioning

confidence: 99%

In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taurolidine is an effective antibacterial substance [1][2][3][4][5][6][7][8] utilized to counteract catheterrelated infections [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. The substance is further effective against malignancy both, in vivo and in vitro [25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Taurolidine Sensitivity of Eryptosis, the Suicidal Erythrocyte Death

Fink

Bhuyan

Zacharopoulou

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: The taurine derivative Taurolidine is effective against diverse bacteria and tumor growth. In the treatment of cancer, the substance is effective in part by triggering suicidal death or apoptosis of tumor cells. The Taurolidine-induced apoptosis involves mitochondria. Erythrocytes lack mitochondria but are nevertheless able to enter suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Signaling of eryptosis includes increase of cytosolic Ca²⁺ activity ([Ca²⁺]_i), oxidative stress and ceramide. The present study explores, whether Taurolidine induces eryptosis and, if so, which cellular mechanisms are involved. Methods: Phosphatidylserine exposure at the cell surface was estimated using annexin-V-binding, cell volume using forward scatter, [Ca²⁺]_i using Fluo3-fuorescence, reactive oxygen species (ROS) formation using 2’,7’-dichlorodihydrofuorescein (DCF)-dependent fluorescence, and ceramide abundance using specific antibodies. Results: A 48 hours exposure of human erythrocytes to Taurolidine (60 µg/ml) significantly enhanced the percentage of annexin-V-binding cells, significantly decreased forward scatter and significantly increased Fluo3-fluorescence and ceramide abundance, but not DCF-fluorescence. The effect of Taurolidine on annexin-V-binding was virtually abrogated by removal of extracellular Ca²⁺. Conclusion: Taurolidine triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect at least in part due to Ca²⁺ entry and paralleled by increase of ceramide abundance.

show abstract

“…One percent of taurolidine inhibited clearly the formation of 12species biofilms; however, the effect on an established biofilm was as limited as shown as for minocycline [17]. In an ex-vivo model using subgingival biofilm samples from periodontitis patients, the decrease of bacterial counts in biofilms was 0.87 log10 cfu, corresponding 86.5% following application of 3% taurolidine gel after 60 min [18].…”

Section: Introductionmentioning

confidence: 84%

Taurolidine acts on bacterial virulence factors and does not induce resistance in periodontitis-associated bacteria

Radakovic

Schmid

Andreoli

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Background Taurolidine is thought to be an alternative antimicrobial in periodontal therapy. The purpose of this follow-up taurolidine study was to determine in more detail the mode of action of taurolidine against bacterial species being associated with periodontal disease. Further, a potential development of resistance against taurolidine in comparison with minocycline was to evaluate. Results Visualizing the mode of action of taurolidine to Porphyromonas gingivalis by scanning and transmission electron micrographs showed in part pores and release of constituents from the cell wall. The interaction of taurolidin with bacterial cell wall is also supported by the finding that taurolidine inhibited in a concentration-dependent manner the activities of LPS and of the P. gingivalis arginine-specific gingipains. However, an effect on A. actinomycetemcomitans leukotoxin was not found. When transferring 14 clinical isolates from subgingival biofilm samples (4 P. gingivalis, 2 A. actinomycetemcomitans, 2 Tannerella forsythia, 2 Fusobacterium nucleatum, 4 oral streptococci) on agar plates containing subinhibitory concentrations of taurolidine up to 50 passages, one P. gingivalis strain developed a resistance against taurolidine which was probably linked with efflux mechanisms. When antimicrobial pressure was removed, MIC reverted to baseline value. Testing development of resistance to minocycline in a similar way, an increase of MIC values occurred in five of the 14 included strains after exposure to subinhibitory concentrations of the antibiotic. Efflux might play a role in one A. actinomycetemcomitans strains, but obviously not in the other four strains. Removing antimicrobial pressure for a few passages did not revert the increased MIC values. Conclusion Taurolidine interacts with LPS and gingipains. Development of resistance seems to be a rare event when applying taurolidine. A potential development of resistance might be associated with efflux mechanisms.

show abstract

Activity of taurolidine gels on ex vivo periodontal biofilm

Abstract: Within their limits, the data suggest that TAU 3% gel might represent a potential alternative to 0.2% chlorhexidine gel.

Cited by 15 publications

References 50 publications

In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

Taurolidine Sensitivity of Eryptosis, the Suicidal Erythrocyte Death

Taurolidine acts on bacterial virulence factors and does not induce resistance in periodontitis-associated bacteria

Contact Info

Product

Resources

About