Activity of South African medicinal plants against Listeria monocytogenes biofilms, and isolation of active compounds from Acacia karroo

Nyila, Monde Alfred; Leonard, Carmen M.; Hussein, Ahmed A.; Lall, Namrita

doi:10.1016/j.sajb.2011.09.001

Cited by 45 publications

(30 citation statements)

References 52 publications

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“…Previous reports have suggested that epicatechin was unable to inhibit biofilm formation as observed for Eikenella corrodens, a periodontopathogenic bacterium (Matsunaga et al, 2010) and for P. aeruginosa PAO1 biofilm formation after 18 h of growth (Vandeputte et al, 2010). Similarly, Nyila et al (2012) observed that epicatechin from Acacia karroo did not reduce Listeria monocytogenes biofilms. The observed anti-biofilm activity of epicatechin was, however, strain-specific as adhesion of β-lactam resistant E. coli ATCC 35218 and methicillin-resistant S. aureus ATCC 43300 was increased at all concentrations tested (Fig.…”

Section: Biofilm Microtitre Plate Assaysmentioning

confidence: 86%

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of Ficus Sansibarica Warb. Subsp. Sansibarica (Moraceae) Extracts

Awolola¹,

Koorbanally²,

Chenia³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Ficus species are used in African traditional medicine in the treatment of a wide variety of ailments and diseases such as convulsive disorder, wound healing, gonorrhea, tuberculosis, diabetes, diarrhoeal infections, dysentery, malaria and HIV. The aim of this study was to isolate the phytochemical constituents in the plant and test them for their antibacterial activity. Materials and methods: The fruits, leaves and stem bark were extracted with organic solvents and the compounds in the extracts separated and purified by column chromatography before being identified by NMR spectroscopy and by comparison of the NMR data against values reported in the literature. The antibacterial activity of the pure compounds and extracts were tested using the disk diffusion method. Results: Three triterpenes and three flavonoids: lupeol acetate (1); cycloart-23-ene-3,25-diol (2); β-sitosterol (3); 5,7,4'-trihydroxyflavan-3-ol (4); epicatechin (5); and isovitexin (6) were isolated in this study. Antimicrobial activity was observed at 8 mg mL -1 for Staphylococcus aureus ATCC 29213 with four of the six isolated compounds, with no activity being observed at 1 -4 mg mL -1 against Escherichia coli ATCC 25922, E. coli ATCC 35218 and S. aureus ATCC 43300. Epicatechin (5) was found to decrease adhesion of E. coli ATCC 25922 and S. aureus ATCC 29213. Decreased adhesion of S. aureus ATCC 29213 was also observed with 5,7,4'-trihydroxyflavan-3-ol (4) and isovitexin (6). Conclusions:The results of this study provide baseline information on F. sansibarica's potential validity in the treatment of infections associated with Gram-positive microorganisms.

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Section: Biofilm Microtitre Plate Assaysmentioning

confidence: 86%

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of Ficus Sansibarica Warb. Subsp. Sansibarica (Moraceae) Extracts

Awolola¹,

Koorbanally²,

Chenia³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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“…On the contrary, some compounds (α-pinene, 1,8-cineole, (+)limonene, linalool, and geranyl acetate) enhanced biofilm growth in vitro [10]. Essential oil components from orange peels [11] and a lot of other substances, including epicatechin, β-sitosterol, and epigallocatechin from Acacia karroo [12] and resveratrol [13], were evaluated successfully. Thymol has been also screened as an alternative to conventional antibiotics to inhibit biofilm formation and it was found to be effective against biofilms formed by Staphylococcus aureus and Staphylococcus epidermidis [14].…”

Section: Introductionmentioning

confidence: 99%

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Cusimano¹,

Stefano²,

Giglia

et al. 2020

Molecules

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The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting.

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“…In recent times, the study of medicinal plants has become the focus of ever more extensive research all over the world, due to the diversity and potential that medicinal plants have as source of medicinal products (Ahmad and Beg, 2001;Lee et al, 2008;Rokaya et al, 2010;Street, 2012;Sharma et al, 2012;Shanmugam et al, 2012). Generally, studies related to therapeutic plants aim to characterize the active compounds of the plant for scientific evidence of their therapeutic properties (Naidu et al, 1999;Balunas and Kinghorn, 2005;Gambari and Lampronti, 2006;Jäger et al, 2010;Nyila et al, 2012). However, studies of the distribution of 238 U and 232 Th natural radionuclides series in such plants are sparse.…”

Section: Introductionmentioning

confidence: 99%

Natural radioactivity determination in samples of Peperomia pellucida commonly used as a medicinal herb

Sussa

Damatto

Alencar

et al. 2013

Journal of Environmental Radioactivity

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Activity of South African medicinal plants against Listeria monocytogenes biofilms, and isolation of active compounds from Acacia karroo

Cited by 45 publications

References 52 publications

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of <i>Ficus Sansibarica</i> Warb. <i>Subsp. Sansibarica</i> (Moraceae) Extracts

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of <i>Ficus Sansibarica</i> Warb. <i>Subsp. Sansibarica</i> (Moraceae) Extracts

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Natural radioactivity determination in samples of Peperomia pellucida commonly used as a medicinal herb

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