Activity of Purified Bacterial Myrosinase and its Essential Residues

El-Shora, Hamed M.; Elshobaky, Ahmed; El-Atrozy, Maissa M.

doi:10.20546/ijcmas.2016.502.064

Cited by 8 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…None of these 17 bacterial strains at subspecies level have ever been reported as GSL metabolizers and/or ITC producers, yet they shared the same genus or the same species as previously identified ones. Prior findings showed a variety of GSL-metabolizing bacterial strains such as Bacillus thuringiensis [ 21 ], Actinomycetes isolated from cotton soil [ 22 ], E. coli VL8, Ec. casseliflavus CP1 isolated from human faeces [ 23 ], Lb.…”

Section: Resultsmentioning

confidence: 99%

Formation of Sulforaphane and Iberin Products from Thai Cabbage Fermented by Myrosinase-Positive Bacteria

et al. 2018

View full text Add to dashboard Cite

Myrosinase-positive bacteria from local fermented foods and beverages in Thailand with the capacity to metabolize glucosinolate and produce isothiocyanates (ITCs) were isolated and used as selected strains for Thai cabbage fermentation. Enterobacter xiangfangensis 4A-2A3.1 (EX) from fermented fish and Enterococcus casseliflavus SB2X2 (EC) from fermented cabbage were the two highest ITC producers among seventeen strains identified by 16S rRNA technique. EC and EX were used to ferment Thai cabbage (Brassica oleracea L. var. capitata) containing glucoiberin, glucoraphanin and 4-hydroxyglucobrassicin at 430.5, 615.1 and 108.5 µmol/100 g DW, respectively for 3 days at 25 °C. Different amounts of iberin nitrile, iberin, sulforaphane and indole 3-acetonitrile were produced by spontaneous, EX- and EC-induced cabbage fermentations, and significantly higher ITCs were detected (p < 0.01) with increased antioxidant activities. Iberin and sulforaphane production in EX-induced treatment peaked on day 2 at 117.4 and 294.1 µmol/100 g DW, respectively, significantly higher than iberin at 51.7 µmol/100 g DW but not significantly higher than sulforaphane at 242.6 µmol/100 g DW in EC-induced treatment at day 2. Maximum health-promoting benefits from this functional food can be obtained from consumption of a liquid portion of the fermented cabbage with higher ITC level along with a solid portion.

show abstract

Section: Resultsmentioning

confidence: 99%

Formation of Sulforaphane and Iberin Products from Thai Cabbage Fermented by Myrosinase-Positive Bacteria

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In other studies, only purified protein extracts of different strains showed myrosinase activity even if a specific enzyme was not purified [ 17 , 18 ]. Finally, a myrosinase from a non-gut Bacillus thuringensis strain was partially purified and characterised by El Shora et al (2016) [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

The Metabolism of Glucosinolates by Gut Microbiota

Sikorska-Zimny

Beneduce

2021

Nutrients

View full text Add to dashboard Cite

Glucosinolates (GLS) and their derivatives are secondary plant metabolites abundant in Brassicaceae. Due to the enzymatic reaction between GLS and myrosinase enzyme, characteristic compounds with a pungent taste are formed, used by plants to defend themselves against insect herbivores. These GLS derivatives have an important impact on human health, including anti-inflammation and anti-cancer effects. However, GLS derivatives’ formation needs previous enzymatic reactions catalyzed by myrosinase enzyme. Many of the brassica-based foods are processed at a high temperature that inactivates enzymes, hindering its bioavailability. In the last decade, several studies showed that the human gut microbiome can provide myrosinase activity that potentially can raise the beneficial effects of consumption of vegetables rich in GLS. The variability of the human gut microbiome (HGM) in human populations and the diverse intake of GLS through the diet may lead to greater variability of the real dose of pro-healthy compounds absorbed by the human body. The exploitation of the genetic and biochemical potential of HGM and correct ecological studies of both isolated strains and mixed population are of great interest. This review focuses on the most recent advances in this field.

show abstract

“…GA 3 as growth regulator induced the activity of α-glucosidase. GA 3 induced the activities of other enzymes such as phosphoenol pyruvate carboxy lase [6], myrosinase [7], NADH-glutamate synthetase [8], sucrose synthase [9] and acid phosphatase [10]. The enhancement of α-glucosidase by GA3 might be attributed to enhancing of α-glucosidase synthesis through controlling the transcription and translation.…”

Section: Resultsmentioning

confidence: 99%

Induction, immobilization, modification and natural inhibitors of α-glucosidase from Penicillum chrysogenum

El-Shora

Messgo

Ibrahim

et al. 2019

ijpm

Self Cite

View full text Add to dashboard Cite

α-glucosidase (EC: 3.2.1.20) was isolated from Penicillum chrysogenum. The enzyme was enhanced by plant growth regulators such as gibberellic acid (GA3), benzylaminopurine (BAP) and kinetin. Dansyl chloride inhibited the enzyme at 1, 2, 3, 4 and 5 mM with T0.5 67, 52.2, 34.4 and 23.3 min, respectively. The substrate offered partial protection for the enzyme against dansyl chloride inhibition. The enzyme was activated by Ca2+ and Mg2+. However, Pb2+, Cd2+, Zn2+, Ni2+ and Hg2+ inhibited α-glucosidase activity. The enzyme was immobilized on Ca alginate and the optimal concentration for 3% w/v. The optimal concentration of CaCl2 was recorded at 3 mM. The optimal CaCl2 concentration and the optimum time for immobilization was 3mM and 4hr. The enzyme was inhibited by aqueous extracts of Datura stramonium, Trigonella foenum-graecum, Hyoscymus muticus and Cynodon dactylon. The IC50 values for the four extracts were 59.1, 73.6, 68.5 and 77.1 µg ml-1, respectively.

show abstract

Activity of Purified Bacterial Myrosinase and its Essential Residues

Cited by 8 publications

References 0 publications

Formation of Sulforaphane and Iberin Products from Thai Cabbage Fermented by Myrosinase-Positive Bacteria

Formation of Sulforaphane and Iberin Products from Thai Cabbage Fermented by Myrosinase-Positive Bacteria

The Metabolism of Glucosinolates by Gut Microbiota

Induction, immobilization, modification and natural inhibitors of α-glucosidase from Penicillum chrysogenum

Contact Info

Product

Resources

About