Activity of phenol oxidases at the puparium formation stage in development of nineteen lozenge mutants of Drosophila melanogaster

Peeples, E. Edward; Geisler, Ann; Whitcraft, Carol J.; Oliver, Clarence P.

doi:10.1007/bf00485477

Cited by 21 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lz locus also modifies the levels of phenoloxidase activity. This study confirms that enzyme activity is absent in lz rfg (Warner et al 1974) and lz s larvae (Peeples et al 1969). Crystal cells do not differentiate in these lz alleles (Rizki and Rizki 1981).…”

Section: Discussionsupporting

confidence: 72%

“…The structural gene for PHOX is located on the second chromosome at 80.6, but the structural genes for the A components have not been identified. A number of genes, such as tyrosinase-1 (tyr-1; 2-52.4), lozenge (lz; 1-27.7) and speck (sp; 2-107) affect the activity levels of both the A components and PHOX (Lewis and Lewis 1963;Peeples et al 1969;Warner et al 1975;Batterham and McKechnie 1980). During a survey ofphenoloxidase activity in lz mutants we found an electrophoretic mobility variant of the A3 component.…”

Section: Introductionmentioning

confidence: 87%

See 1 more Smart Citation

Genetics of a Drosophila phenoloxidase

Rizki

Bellotti

1985

Molec. Gen. Genet.

View full text Add to dashboard Cite

An electrophoretic mobility variant of phenoloxidase in a lz stock of Drosophila melanogaster was identified as the A3 component of the phenoloxidase complex by using two different activators to study enzyme activity-natural activator isolated from pupae and 50% 2-propanol. The structural gene for the A3 proenzyme, Dox-3, was not associated with lz on the X chromosome; it mapped to the right of rdo (53.1) and left of M(2)m in the second linkage group. The lz locus affects the differentiation of the crystal cell, the type of hemocyte that carries prophenoloxidase(s) in paracrystalline form. Alleles of lz lacking paracrystalline inclusions in their hemocytes do not have phenoloxidase activity whereas alleles with paracrystalline inclusions have enzyme activity. The presence of proenzyme in the paracrystalline inclusions was demonstrated by in situ activation with natural activator or propanol followed by incubation in buffered dopa.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 87%

Genetics of a Drosophila phenoloxidase

Rizki

Bellotti

1985

Molec. Gen. Genet.

View full text Add to dashboard Cite

show abstract

“…Crystal cells express DoxA1 and CG8193 while lamellocytes express exclusively DoxA3, a strong indication that Dox3 participates in the encapsulation that accompanies melanisation. Melanisation is diminished in the domino mutant that lacks haemocytes (Braun et al, 1998) and in the Black cells (Bc) mutant with aberrant crystal cells (Rizki et al, 1980; Corbo and Levine, 1996) and the Lz knockout, which is devoid of crystal cells (Peeples et al, 1969). One serine protease Sp7 has been reported to be involved in PPO activation and expressed also in the crystal cells (Castillejo-López and Häcker, 2005).…”

Section: Introductionmentioning

confidence: 99%

Drosophila as a model to study the role of blood cells in inflammation, innate immunity and cancer

Wang

Kounatidis

Ligoxygakis

2014

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Drosophila has a primitive yet effective blood system with three types of haemocytes which function throughout different developmental stages and environmental stimuli. Haemocytes play essential roles in tissue modeling during embryogenesis and morphogenesis, and also in innate immunity. The open circulatory system of Drosophila makes haemocytes ideal signal mediators to cells and tissues in response to events such as infection and wounding. The application of recently developed and sophisticated genetic tools to the relatively simple genome of Drosophila has made the fly a popular system for modeling human tumorigensis and metastasis. Drosophila is now used for screening and investigation of genes implicated in human leukemia and also in modeling development of solid tumors. This second line of research offers promising opportunities to determine the seemingly conflicting roles of blood cells in tumor progression and invasion. This review provides an overview of the signaling pathways conserved in Drosophila during haematopoiesis, haemostasis, innate immunity, wound healing and inflammation. We also review the most recent progress in the use of Drosophila as a cancer research model with an emphasis on the roles haemocytes can play in various cancer models and in the links between inflammation and cancer.

show abstract

“…Other lz mutants showed intermediate activity levels. This pattern closely parallels levels of A1 activity in these mutants (Peeples et al, 1969).…”

Section: Genetic Control Of Phox Activity Levelsmentioning

confidence: 58%