Activity of MMP‐9 after repair of abdominal wall defects with acellular and crosslinked bovine pericardium in rabbit

Abstract

This study was undertaken for the identification of matrix metalloproteinases (MMPs) in extracts obtained from native, acellular and crosslinked bovine pericardium (in vitro), as well as in the plasma after implantation of these biomaterials in rabbits (in vivo). Native pericardium (NP) expressed a 72 kDa (MMP-2) band; whereas, in acellular pericardium (AP) two bands (10 kDa and 92 kDa) of MMPs were observed of which, 92 kDa band was very faint. AP crosslinked with glutaraldehyde did not show any gelatinase activity and thus reflects the creation of new additional chemical bonds between the collagen molecules which has been effectively removed. Gelatin zymography showed only one major band of 92 kDa in all the implanted and untreated rabbit plasma, but the relative amount of 92 kDa was 1-2 times higher in acellular bovine pericardium implanted rabbits as compared to crosslinked and native groups. In NP group, the 92 kDa band was the dullest among the three groups. This indicated that the level of MMP-9 corresponds to the degree of collagen degradation.

A novel wound dressing material for full-thickness skin defects composed of a crosslinked acellular swim bladder

“…The results of animal experiments showed that the total IgG response in rabbit serum of the EDC group was significantly reduced compared to that of the no crosslinking group (p < 0.05); the ELISA experiment also confirmed this conclusion (Kumar et al, 2015). Similarly, a reduced total IgG responses was also observed in rabbit serum implanted subcutaneously with glutaraldehyde crosslinked bovine pericardium, compared with un-crosslinked bovine pericardial tissue (Singh et al, 2014). No adverse immune reactions were observed in rabbits during the period of this study.…”

Addition of platelet‐rich plasma supports immune modulation and improved mechanical integrity in Alloderm mesh for ventral hernia repair in a rat model

“…Previous studies also suggest that a pharmacologically induced immunosuppressed state is associated with fewer complications and hernia recurrence when Alloderm is used for repair (Brewer et al, 2011; Misra et al, 2008). As Alloderm is largely a matrix of collagen and elastin, the presence of chronic inflammatory cells can be detrimental to its longevity due to factors involved in remodeling, such as MMPs that are part of the inflammatory enzymatic milieu (Carlson, Lee, & Pierce, 2013; Han et al, 2009; Lindman et al, 2006; Nagase, Visse, & Murphy, 2006; Singh et al, 2014). The ability of PRP to quell the inflammatory degradation of the graft is evidenced by the preservation of ADM thickness in the experimental arm compared with the control arm (Figure 3), and indeed our data suggest this may be a result of reduced MMP expression and increased Timp1 in PRP‐treated mesh (Figure 6).…”