Activity of MMP-2, MMP-8 and MMP-9 in serum as a marker of progression of alcoholic liver disease in people from Lublin Region, eastern Poland

Abstract

In alcoholic liver cirrhosis, normal liver cells are replaced by scar tissue (fibrosis). Liver fibrosis is a dynamic process in which activated hepatic stellate cells are involved in the synthesis of matrix proteins and the regulation of matrix degeneration. The aim of the presented study was to assess the usefulness of MMP-2, MMP-8 and MMP-9 as diagnostic markers of alcoholic liver disease. Sixty patients with alcoholic liver cirrhosis were randomly enrolled during hospitalization in departments of hospitals from the Lublin Region in eastern Poland. The stage of cirrhosis was estimated according to Child-Turcotte-Pugh criteria (Child-Pugh score) as P- Ch A, P-Ch B, P-Ch C. The control group consisted of 10 healthy persons without liver disease, who did not drink alcohol. Additionally, a group of alcoholics without liver cirrhosis was included in the study. Blood sample were obtained, and after centrifuge, serum was collected for further analysis. The activity of MMP-2, MMP-8 and MMP-9 in the blood plasma of the patients and the control group were measured by using the sandwich enzyme immunoassay technique with commercially available quantitative ELISA test kits. Activity of MMP-2, MMP-8 and MMP-9 in patients with liver cirrhosis were increased gradually according to Child-Pugh stages. The activity of MMP-2, MMP-8, MMP-9 were the highest in patients with liver cirrhosis stage C. MMP-2, MMP-8, MMP-9 concentrations in the people with liver cirrhosis (stage C) were significantly increased compared to controls. A significant difference were observed between activity MMP-2 in control group, alcoholics without liver cirrhosis, and those with liver cirrhosis (stages A, B, C according Child-Pugh score). MMP-2, MMP-8 and MMP-9 may be markers of alcoholic liver cirrhosis in the alcoholics. Elevated levels of MMP-2, MMP-8 and MMP-9 in the alcoholic patients indicated that cirrhosis has developed. The most sensitive is MMP-2, because the activity of this parameter is increased in all liver cirrhosis stages. MMP-8 and MMP-9 activity were significantly elevated only in serum patients with advanced liver cirrhosis, compared to controls.

<p>Carvedilol attenuates carbon tetrachloride-induced liver fibrosis and hepatic sinusoidal capillarization in mice</p>

“…In addition, MMP-8 was considered to promote collagen breakdown 42. The data confirmed that, along with more HSCs were activated in CCl 4 -treated mice, the level of MMP-8 in these mice was increased to degrade the collagen.…”

Hepatitis C Virus as risk factor for development of hepatocellular carcinoma in Egypt: II-Enhancement role of matrix metalloproteinases-2 in dissemination of HCC

“…Our finding that MMP-2 levels were elevated also in non-HCV cirrhotics is in accordance with recent observation by Prystupal et al, (2015) (32) who found that MMP-2 activity in sera of patients with different stages of alcoholic liver cirrhosis was significantly increased compared to the control group. They showed also that among other MMPs, MMP-2 has been found to be the most sensitive as its activity was increased in all stages of liver cirrhosis.…”

Gelatinasas como marcadores de consumo crónico de alcohol: un estudio piloto en Uruguay

“…Si bien Sillanaukee y colegas (2002) (18) mostraron elevaciones de proteína MMP-9 en alcohólicos finlandeses probablemente debido a la condición inflamatoria general crónica que sufren los alcohólicos (11) , los aumentos eran menores y no habían reportado cambios en MMP-2. Analizando pacientes del este de Polonia, Prystupa y colegas (2015) (29) encontraron niveles aumentados de MMP-2, MMP-8 y MMP-9 solo en las etapas cirróticas en alcohólicos cirróticos hepáticos, pero no antes. En cambio, nuestros resultados no están de acuerdo con los datos obtenidos por Madro y cols.…”