Activity of Lactate Dehydrogenase in the Course of Experimental Trichinellosis In Guinea Pigs

Abstract

Activity of the total plasma LDH has been determined in the course of trichinellosis in experimentally infected guinea pigs. Blood samples were processed by a modified micromethod using the SEVATEST LDH outfit. Enzymatic activity was determined and limits of confidence were calculated to evaluate results.In uninfested guinea pigs, the mean value for total LDH activity was 164.02 ± ± 39.84 lUlL. Differences were observed in connection with age and sex. Following infestation by Trichinella larvae, the LDH activity increased gradually till 6th week, reaching so its peak when the period of migration was completed and the larvae definitely settled in muscles:Lactate dehydrogenase, trichinellosis.

Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (<i>Crocodylus niloticus</i>) experimentally infected with <i>Trichinella zimbabwensis</i>

“…In guinea pigs infected with Trichinella spp. larvae, LDH continued to increase until Day 42 PI (Koudela & Schanzel 1980), which was similar to the results observed in the low-infection group in the current study. The continued increase in LDH observed in the medium-infection and high-infection groups once again suggests that larval development and subsequently larval migration may have been delayed in these groups.…”

“…The differences in time to reach peak LDH and AST values observed between the experimental groups suggest that these enzymes are not necessarily indicators of muscle damage. The kinetics of these enzymes appears to correspond better with the migration of larvae rather than with actual damage associated with the larvae, this is in agreement with the hypothesis of Koudela and Schanzel (1980).…”

“…Although the mean increase in LDH values appeared to be correlated with infection intensity, no direct correlation between these two factors could be established. Koudela and Schanzel (1980) reported that the relationship between levels of LDH and infection intensity was not always apparent when comparing individual animals of the same group. In guinea pigs infected with Trichinella spp.…”

“…Increased serum levels of ALT in pigs infected with T. spiralis have also been previously reported (Ribicich et al 2007). However, elevated serum levels of these enzymes are not necessarily indicative of Trichinella infection, as there may be other causes (Koudela & Schanzel 1980;Ribicich et al 2007;Srivastava & Chosdol 2007;Tassi et al 1995;Wisniewska 1970). Comparisons between rats and human patients indicated that an increase in enzyme levels is dependent on the individual response from the host rather than being correlated with intensity of infection or clinical severity (Wisniewska 1970).…”