Activity of<i>α</i>-1,4-Glucosidase in Human Serum

Gamklou, R; Scherstén, T

doi:10.3109/00365517309082413

Cited by 13 publications

(1 citation statement)

References 21 publications

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“…Due to the extremely low ctivity of both enzymes in human serum (5)(6)(7)(8)(9) and to the high molecular weight of aglucosidases (10)(11)(12)(13) it is reasonable to assume that neither α-glucosidases are filtered to a significant extent in the renal glomerulus. The ctivity of neutral α-glucosidase originales from brush border membranes of renal proximal cells (14), whereas acid aglucosidase originales from lysosomes (15).…”

Section: Introductionmentioning

confidence: 99%

Excretion of Neutral α-Glucosidase, Determined with a Continuous Assay, and of Acid α-Glucosidase in the Urine of Human Reference Subjects

Pape¹,

Kochmann²,

Kochmann³

et al. 1983

Clinical Chemistry and Laboratory Medicine

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The catalytic activities of neutral and acid a-glucosidase were selectively determined in human urine. Urinary excretion of neutral and acid α-glucosidase in reference subjects was found to be in the r nge 1.61 to 20.36 μίςαί/πιοί creatinine and 7.47 to 33.60 μkat/mol creatinine, respectively. Urinary excretion of both enzymes was not related to sex, age or diuresis. A continuous assay was introduced to improve the determination of neutral a-glucosidase. Bestimmung der Ausscheidung von neutraler α-Glucosidase mit einem kontinuierlichen optischen Test und von saurer α-Glucosidase im Urin von ReferenzpersonenZusammenfassung: Die Aktivit t von neutraler und saurer α-Glucosidase wurde selektiv im menschlichen Urin bestimmt. Im Urin von Referenzpersonen wurde eine Ausscheidung von neutraler α-Glucosidase zwischen 1,61 und 20,36 μkat/mol Kreatinin und von saurer α-Gl cosidase zwischen 7.47 und 33,60 μkat/mol Kreatinin gemessen. Die Ausscheidung beider Enzyme war weder geschlechts-, alters-noch diureseabh ngig. Zur Optimierung der Bestimmung von neutraler α-Glucosidase wird ein kontinuierlicher optischer Test eingef hrt.

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Section: Introductionmentioning

confidence: 99%

Excretion of Neutral α-Glucosidase, Determined with a Continuous Assay, and of Acid α-Glucosidase in the Urine of Human Reference Subjects

Pape¹,

Kochmann²,

Kochmann³

et al. 1983

Clinical Chemistry and Laboratory Medicine

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Late-Onset Acid Maltase Deficiency

Mehler

DiMauro

1976

Arch Neurol

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Daily urinary excretion of acid maltase (12.78 +/- 2.10 units/24 hr/mg of creatinine, in 11 normal adults) was significantly decreased in ten patients with late-onset acid maltase deficiency (1.33 +/- 0.16 units/24 hr; P less than .001) and 11 heterozygotes (3.27 +/- 0.62 units/24 hr; P less than .001). Maximal inhibition of urinary acid maltase activity by antibodies against human placental enzyme was 53% in controls, 30% in heterozygotes, and virtually absent in patients. Investigation of pH curves and enzyme inhibition by antibodies confirmed the presence in the kidney of an immunologically distinct "extra" maltase enzyme active at acid pH. Whether acid maltase in normal urine originates in the kidney or cells of the lower urinary tract, the enzyme defect seems to be expressed in these cells in late-onset acid maltase deficiency.

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Activity of α-1,4-glucosidase in serum from patients with malignant tumor

Gamklou¹,

Scherstén²

1973

Cancer

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Activity ofα-1,4-Glucosidase in Human Serum

Cited by 13 publications

References 21 publications

Excretion of Neutral α-Glucosidase, Determined with a Continuous Assay, and of Acid α-Glucosidase in the Urine of Human Reference Subjects

Excretion of Neutral α-Glucosidase, Determined with a Continuous Assay, and of Acid α-Glucosidase in the Urine of Human Reference Subjects

Late-Onset Acid Maltase Deficiency

Activity of α-1,4-glucosidase in serum from patients with malignant tumor

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