Activity of Glucose Isomerase from Bacillus thuringiensis under Different Treatments

Abstract

Various microorganisms have been found to produce glucose isomerase (Deshmukh et al., 1994;Belfaquih and Penninckx., 2000;Givry and Duchiron, 2007). The conversion of glucose to fructose chemically has been known to demand high pH and temperature. The possibility of producing fructose chemically from glucose has been studied by Barker et al. (1973).

Induction, immobilization, modification and natural inhibitors of α-glucosidase from Penicillum chrysogenum

“…The activation of α-glucosidase by Ca 2+ could be due to the in forcing of the interactions inside α-glucosidase molecules and by the binding of Ca 2+ to the autolysis site. Ca 2+ activated other enzymes such as phytase [17] and glucose isomerase [18].…”

Production, Characterization and Optimal Performance Studies of Glucose Isomerase by Achromobacter xylosoxidans mck-4 Isolated from Starch Milling Wastes

“…Glucose isomerase produced from Actinoplanes missouriensis exhibited Km value of 1.33 moles for glucose [15]. The Km reported by El-Shora et al [24] from Bacillus thuringiensis was 8 mM for glucose. Dhungel et al [4] reported 0.45 M as Km and 0.18 U/mg as Vmax for glucose by using psychrotolerant Streptomyces sp.…”

“…GI from Thermoanaerobacter ethanolicus was found to isomerize best at 85°C by Fan et al [27] and the enzyme produced by Thermotogane apolitana was extremely thermostable with an optimum temperature at 95°C [31]. El-Shora et al [24] found out that glucose isomerase from Bacillus thuringiensis isomerizing best at 50°C. Lee et al [32] observed the optimal activity of GI from Dunaliellater tiolecta to be 60°C.…”

“…Ogbo and Odibo [13] found Saccharococcus caldoxylosilyticus exhibiting maximum activity at pH 6.4, Sriprapundh et al [34] found an optimum activity for Thermotogane apolitana at pH 7, Lama et al [29] Bacillus thermoantarticus pH 7. El-Shora et al [24] found their glucose isomerase from Bacillus thuringiensis isomerizing best at pH of 7.0. Lee et al [32] reported pH of 6.0 as optimum for Dunaliellater tiolecta.…”

“…Glucose isomerases from different microbial sources responded differently to the presence of divalent cations. El-Shora et al [24] found their glucose isomerase from Bacillus thuringiensis requiring Mn²⁺ and Ca²⁺ as cofactors. Yanmis et al [30] stated divalent cations Co²⁺, Mg²⁺, and Mn²⁺ were essential for xylose isomerase produced by Anoxybacillus gonensis whereas bivalent metal ions Ca²⁺, Hg²⁺, Ni²⁺, Zn²⁺, Fe²⁺ and Cu²⁺ showed inhibitory effects.…”