Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals

Ghonaim, Mabrouk; Makled, Amal F.; Sharweed, Moustafa

doi:10.21608/ejmm.2020.249865

Cited by 1 publication

(2 citation statements)

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“…In this study 180 Gram negative bacilli were isolated from 310 collected urine samples representing 58%. Similar was reported by Gharieb et al, 23 in Egypt found that out of 155 studied patients, 99 (64%, n= 99) isolates were Gram negative bacilli. Several studies reported more than 50 % of their CAUTI were caused by Gram negative organisms.…”

Section: Discussionsupporting

confidence: 88%

“…In our study, (86.7%, n= 156) strains were MDR, and most of them (60.9%, n=95) were isolated from ICU, and (39.7%, n=62) of them were E. coli which represents (82,7%, n=62) of all E. coli isolates. Gharieb et al, 23 11 in Iran. The difference in the rate of resistance to fosfomycin may be due to the amount of antibiotic usage in the different area.…”

Section: Discussionmentioning

confidence: 99%

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Isolation of Gram-Negative Organisms Causing Nosocomial Catheter Associated Urinary Tract Infection and Detection of Fosfomycin Effect on Multi-Drug Resistant Strains in Sohag University Hospital

Salman

Mohamed

Elwafa

et al. 2023

Egyptian Journal of Medical Microbiology

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Background: CAUTIs are the commonest HAIs and frequently caused by MDR Gram negative organisms, fosfomycin could be potentially used for this infection. Objectives:To isolate, identify Gram negative organisms causing CAUTIs, their antibiotic resistance pattern and to investigate their sensitivity to fosfomycin at Sohag University Hospitals. Methodology: Cross-sectional study included patients with catheter associated urinary tract infections (CAUTIs) admitted in the different Departments of Sohag University Hospitals from December 2019 to December 2022. The study included 310 urine samples from CAUTIs. Urine samples were subjected to routine culture, complete biochemical identification and antibiotic sensitivity testing were done by the BD Phoenix ™ automated identification and susceptibility testing system. ESBL producing organisms were detected by double-disk synergy test (DDST), Carbapenemase production by the modified Hodge test (MHT) and MBL production by EDTA combined disk synergy test (CDST). Fosfomycin sensitivity was detected by the disc diffusion method and minimum inhibitory concentration (MIC) by E-test. Conventional PCR was used to detect FosA, fosA2, fosA3, fosC2 genes. Results: The study group included (180) patients from them Gram negative bacilli were isolated. Their mean age was 52.8±11.7 years. Males were 99 (55%) of all studied patients. Most of them were from ICU. The most common isolated organism was E. coli 75 (41.7%), followed by Klebsiella pneumoniae 39 (21.7%), and Enterobacter cloacae 24 (13.3), 156 (86.6%) were MDR, 67 (43.3%) were ESBL producers by DDST, 49 (27%) Carbapenemase producers by MHT and 40 (22%) were MBL producers by EDTA CDST. One hundred and fifteen strains (66%) were sensitive to fosfomycin by E-test and FosA, A2, A3, C2 genes were detected in 47 (72.3%) of fosfomycin resistant strains. Conclusion: The study revealed that 86% of CAUTI were caused by MDR Gram negative organisms and 59% of them were susceptible to fosfomycin.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%