Activity of essential oil of Lippia triplinervis Gardner (Verbenaceae) on Rhipicephalus microplus (Acari: Ixodidae)

Lage, Tiago Coelho de Assis; Montanari, Ricardo Marques; Fernandes, Sérgio Antônio; Monteiro, Caio Márcio de Oliveira; Senra, Tatiane de Oliveira Souza; Zeringóta, Viviane; Calmon, Fernanda; Matos, Renata da Silva; Daemon, Erik

doi:10.1007/s00436-012-3209-y

Cited by 33 publications

(12 citation statements)

References 41 publications

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“…These prevalent compounds were also found in L. sidoides oil analysed in other studies, with proportions varying as a function of cropping area (Oliveira, Moura, Lopes, Andrade, Silva & Figueiredo ; Cavalcanti, Niculau, Blank, Câmara, Araújo & Alves ; Farias‐Junior, Rios, Moura, Almeida, Alves, Blank, Fernandes & Scher ; Mota, Lobo, Costa, Costa, Rocha, Silva, Pohlit & Andrade‐Neto ; Carvalho, Laranjeira, Carvalho‐Filho, Souza, Blank, Alves, Jesus & Warwich ; Veras, Araruna, Costa, Coutinho, Kerntopf, Botelho & Menezes ). In contrast to our findings and most studies on this species, Lage, Montanari, Fernandes, Monteiro, Senra, Zeringota, Calmon, Matos and Daemon () reported carvacrol (31.9%) as the major component and thymol (30.6%) as the second most prevalent component in the essential oil of L. sidoides cropped in Araponga, MG.…”

Section: Resultscontrasting

confidence: 99%

Chemical composition of Lippia spp. essential oil and antimicrobial activity against Aeromonas hydrophila

Majolo¹,

Rocha²,

Chagas³

et al. 2016

Aquac Res

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Phytotherapy can replace antibiotic administration as an alternative to control Aeromonas hydrophila, one of the main bacteria involved in the aetiology of farmed fish diseases. Given that plants of the Lippia spp. genus show biological potential for antimicrobial activity, this study evaluated the chemical composition of essential oils extracted from Lippia alba, Lippia origanoides and Lippia sidoides and their activity against A. hydrophila. The oils were obtained by steam distillation in a Clevenger‐type apparatus and their composition determined by gas chromatography and mass spectrometry (CG/MS). Antibacterial activity was assessed by calculating the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using broth microdilution method. The main compounds identified were geranial (25.4%) and neral (16.6%) in L. alba oil, carvacrol (40.4%) and p‐cymene (11.4%) in L. origanoides oil and thymol (76.6%) and ortho‐cymene (6.3%) in L. sidoides oil. The three Lippia species showed bacteriostatic and bactericidal action against A. hydrophila, with MICs and MBCs ranging from 1250 to 5000 μg mL−1. Of the species tested, the best performance was obtained with essential oil of L. sidoides.

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Section: Resultscontrasting

confidence: 99%

Chemical composition of Lippia spp. essential oil and antimicrobial activity against Aeromonas hydrophila

Majolo¹,

Rocha²,

Chagas³

et al. 2016

Aquac Res

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“…A significant decrease in the egg mass laid by female Rhipicephalus was observed after immersion in the essential oils of L. triplinervis (Lage et al, 2013), geranium (PiraliKheirabadi et al, 2009) and H. ringens (Ribeiro et al, 2010), suggesting that sub-lethal doses of essential oils may have an effect on tick fecundity. The viability of eggs from adults exposed to essential oils may also be affected.…”

Section: Ticksmentioning

confidence: 99%

“…The viability of eggs from adults exposed to essential oils may also be affected. Immersion in oil of L. triplinervis caused a significant decrease in tick egg hatch (Lage et al, 2013).…”

Section: Ticksmentioning

confidence: 99%

“…In addition, in some cases the units used to record the concentrations are not intuitive and therefore it is extremely difficult to compare the efficacy of oils tested in one study with that of oils tested in another. For example, the use of mg/mL as a unit of measurement is surprising in a context in which the diluted product is a liquid and the specific weight is not stated (Ribeiro et al, 2007;Lage et al, 2013). Using this unit of measurement because it refers to the weight of the plant prior to oil extraction is also problematic as different plants and even sections of plants contain vastly different concentrations of essential oil.…”

Section: Ticksmentioning

confidence: 99%

“…Neither of the oils gave 100% adult mortality at the concentrations tested and many had very poor acaricidal efficacy. Essential oil of eucalyptus (Eucalyptus globulus) killed just 37.5% of adult ticks at 6 days after immersion in a 5% solution, and some oils, including those of H. polyanthemum (Ribeiro et al, 2007), C. serrata (Ribeiro et al, 2008) and Lippia triplinervis (Lage et al, 2013), have been shown to have no adult acaricidal effects at all.…”

Section: Ticksmentioning

confidence: 99%

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The use of essential oils in veterinary ectoparasite control: a review

Ellse

Wall

2013

Medical Vet Entomology

120

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There is a growing body of evidence indicating the potential value of essential oils as control agents against a range of arthropod ectoparasites, particularly lice, mites and ticks. Toxicity has been demonstrated following immersion and physical contact with treated surfaces, as well as after exposure to the vapour of these oils; the last of these factors implies that there is a neurotoxic, rather than simply a mechanical, pathway in their mode of action. However, the volatile nature of essential oils suggests that their residual activity is likely to be short-lived. A possible advantage of essential oils over conventional ectoparasite treatments may refer to their reported ovicidal efficacy, although it is unclear whether this results from neurotoxicity or mechanical suffocation. There are many difficulties in comparing the findings of existing studies of essential oil toxicity. One major issue is the wide variation among batches in the relative concentrations of oil constituents. A second issue concerns the fact that many experimental designs make it difficult to confirm that the effect seen is attributable to the oil; in many cases inappropriate controls mean that the effects of the excipient on mortality cannot be distinguished. Hence, it is important that an excipient-only control is always included in these bioassays. Furthermore, in direct contact assays, when attempting to identify the toxicity pathway of the essential oil tested, it is important to include a hydrophobic control. Without this, it is impossible to distinguish simple mechanical effects from neurological or other cellular toxicity. The use of essential oils in the control of veterinary ectoparasites is an area which holds considerable potential for the future and research into their use is still at an early stage. More extensive field trials, the standardization of components, the standardization of extraction, the standardization of good experimental design, mammalian toxicology profiling and excipient development, as well as further investigation into the residual activities and shelf-lives of these oils are all required to allow the full realization of their potential.

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Acaricidal activity of essential oils from five endemic conifers of New Caledonia on the cattle tick Rhipicephalus (Boophilus) microplus

Lebouvier

Hüe²,

Hnawia

et al. 2013

Parasitol Res

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The aim of the present study was to demonstrate acaricidal activity on the cattle tick Rhipicephalus (Boophilus) microplus of essential oils from endemic conifers of New Caledonia in the context of the development of natural alternatives. Acaricidal activity of essential oils extracted from resin and heartwood of five endemic conifers of New Caledonia (Araucaria columnaris, Agathis moorei, Agathis ovata, Callitris sulcata, and Neocallitropsis pancheri) was evaluated on 14- to 21-day-old Rhipicephalus microplus tick larvae using the Larval Packal Test bioassay. A first screening with 5% dilute solution was carried out and the oils with 100% of mortality at this rate were diluted until no activity was shown. The heartwood oils of the two Cupressaceae were the most active with LC50 value of 0.65% for C. sulcata and 0.55% for N. pancheri while resin oil of A. columnaris (LC50=1.62%) was the most active of the Araucariaceae family. Negative control (ethanol) was not toxic to the larvae. The chemical composition of essential oil from resin of A. columnaris was analyzed by gas chromatography-mass spectrometry. The essential oil was characterized by high level of sesquiterpene hydrocarbons and oxygenated sesquiterpenes and was composed mainly of aromadendrene (23.1%) and bicyclogermacrene (16.0%). In order to compare different plant resources in a sustainable program of natural acaricide, an "essential oil efficiency EOE" can be measured as the ratio between the yield of extraction and LC50 value. This study shows that A. columnaris (EOE=2.36) and N. pancheri (EOE=3.51) could provide valuable and effective natural acaricides for control of the cattle tick R. microplus.

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Activity of essential oil of Lippia triplinervis Gardner (Verbenaceae) on Rhipicephalus microplus (Acari: Ixodidae)

Cited by 33 publications

References 41 publications

Chemical composition of Lippia spp. essential oil and antimicrobial activity against Aeromonas hydrophila

Chemical composition of Lippia spp. essential oil and antimicrobial activity against Aeromonas hydrophila

The use of essential oils in veterinary ectoparasite control: a review

Acaricidal activity of essential oils from five endemic conifers of New Caledonia on the cattle tick Rhipicephalus (Boophilus) microplus

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