Activity of eight strains of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five stored product pests

Negrisoli, C. R. de C. B.; Júnior, Aldomário Santo Negrisoli; Bernardi, Daniel; Garcia, Mauro Silveira

doi:10.1016/j.exppara.2013.03.008

Cited by 21 publications

(5 citation statements)

References 22 publications

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“…Total body length of IJ isolated from Viti Levu (520-630 µm) is larger ( Kour et al, 2020 ) then the body length reported for H. indica strain T2 (530µm). IJ needs a thin layer of water on the surface of soil particles for movement and infectivity ( Negrisoli et al, 2013 ). For maximum movement, the layer of water should be as thick as nematode’s body diameter ( Wallace, 1958 ).…”

Section: Discussionmentioning

confidence: 99%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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Entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are obligate parasites of soil inhibiting insects. EPNs are being widely researched as promising biocontrol agents for a wide range of agricultural pests. It is known that strains of EPNs isolated from different geographical regions differ in their attributes, such as host-finding ability, host range, infectivity, reproduction, and environmental stress tolerance. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. Thus, ecological characterisation of the EPN Heterorhabditis indica (Rhabditida: Heterorhabditidae) newly isolated and representing the only species of EPN reported from the island of Viti Levu, Fiji was carried out using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts to allow comparisons between bioassays conducted in different laboratories around the world. Temperature data showed that native isolates of H. indica are warm-adapted nematodes with thermal range for infectivity between 15˚C and 35˚C and can reproduce between 20˚C and 30˚C. They are highly virulent with LC50 values against G. mellonella ranging from 2.8 IJ to 3.8 IJ/larva. However, they showed poor desiccation tolerance and fail to infect hosts in soil with moisture levels below 8%. They showed a moderate level of hypoxic tolerance and can be stored at 15˚C for 4 months. Results also showed great variability within the selected native isolates of H. indica. Beneficial traits for selected isolates were added up to identify a superior candidate. The current study also suggested that the thermal niche breadth for infection can differ among conspecific strains of an EPN species. The results of this experimental study on ecological aspects of these native isolates of H. indica should form a basis for their potential use in biological control of insect pests in Fiji.

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Section: Discussionmentioning

confidence: 99%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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“…Therefore, the discovery of highly virulent EPN strains has further enhanced their efficacy [ 64 ], garnering increased interest in their utilization against stored-grain pests. To date, the EPN, S. carpocapsae , constitutes one of the most effective EPNs used against insect pests [ 67 , 79 , 81 , 82 , 87 ], as it constitutes a vector for enteric entomopathogenic bacteria of the genus Xenorhabdus [ 88 ]. Some of its advantageous characteristics for the management of insect pests as a nematode species are its high fecundity, small size, and short generation time, as well as its safety concerning human health, the environment, and non-target organisms [ 67 , 88 , 89 ].…”

Section: Discussionmentioning

confidence: 99%

Natural Warriors against Stored-Grain Pests: The Joint Action of Beauveria bassiana and Steinernema carpocapsae

Wakil

Kavallieratos

Eleftheriadou

et al. 2023

JoF

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Tribolium castaneum, Trogoderma granarium, Oryzaephilus surinamensis, Sitophilus oryzae, Rhyzopertha dominica, and Cryptolestes ferrugineus are all major pests of stored grains. In this study, the efficiency of single and joint applications of the entomopathogenic nematode (EPN) Steinernema carpocapsae at two different doses (50 and 100 IJs cm−2) and the entomopathogenic fungus (EPF) Beauveria bassiana for the management of the aforementioned pests was estimated. At single treatments, both doses of S. carpocapsae caused higher mortality rates to all six pest species compared to B. bassiana. The combined treatment of EPF and EPN resulted in higher mortality compared to single treatments. Mortality was strongly influenced by the exposure interval and the application dose of the EPN at both single and combined treatments. Maximum mortality was observed for the application of the combined treatment at the high dose of S. carpocapsae and B. bassiana. Among the different insect species tested, the maximum mortality rate was observed for R. dominica (96.62%), followed by S. oryzae (90.48%), T. castaneum (87.23%), C. ferrugineus (76.05%), O. surinamensis (70.74%), and T. granarium (57.71%). The outcomes of this study demonstrate the potential of utilizing specific combinations of EPF and EPN as effective natural enemies against stored-grain pests.

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“…Larvae of T. castaneum were highly susceptible to 3 steinernematid species, i.e., S. feltiae, S. carpocapsae, and S. riobrave, whereas pupae and adults' susceptibility was species dependent (Ramos-Rodríguez et al, 2006a, b). Different EPN species have also been evaluated against S. oryzae, with variable success Salma, 2010 andBarbosa-Negrisoli et al 2013). Most studies suggest that S. oryzae adults are not highly susceptible to EPNs, with mortality levels of 9, 26, or 40% (Ramos-Rodríguez et al, 2006a, b).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)

Khanum

Javed

2021

Egypt J Biol Pest Control

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Background Stored grain products suffer a considerable economic loss due to insect infestations. The resistance of pests to insecticide, and residues on crops are produced by the application of insecticide. Nowadays, incremental necessity has been occurred to find out alternatives to chemicals. As biological control agents, entomopathogenic nematodes (EPNs) attract attention increasingly in the research area recently. Results The aim of the study was to evaluate the pathogenicity of Pakistani isolates Steinernema bifurcatum and S. affine against the stored grain pests, Tribolium castaneum and Lasioderma serricorne, under laboratory conditions. Suspensions of nematodes were applied at 4 different concentrations 0, 50, 100, and 200 IJs/ml and 3 variable temperatures 15, 30, and 45°C. Pathogenicity rate was recorded after 48 h of application. The larval stage is found more susceptible than the adult. At 200 IJs, S. bifurcatum showed high mortality of L. serricorne larva (92%) and T. castaneum larva (93%), whereas S. affine showed 90 and 95% mortality of L. serricorne and T. castaneum larva, respectively, at 200 IJs. The results revealed that both species of EPN were able to control and reproduce on L. serricorne and T. castaneum. Maximum mortality was recorded at 45 °C from S. bifurcatum whereas S. affine at 15 °C. Conclusions Therefore, S. bifurcatum and S. affine could be suggested as a biological control agent for hot and cold climatic zones, respectively.

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Activity of eight strains of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five stored product pests

Cited by 21 publications

References 22 publications

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Natural Warriors against Stored-Grain Pests: The Joint Action of Beauveria bassiana and Steinernema carpocapsae

Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)

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