Activity of disinfectants against multispecies biofilms formed byStaphylococcus aureus,Candida albicansandPseudomonas aeruginosa

Kart, Didem; Tavernier, Sarah; Acker, Heleen Van; Nelis, Hans; Coenye, Tom

doi:10.1080/08927014.2013.878333

Cited by 44 publications

(35 citation statements)

References 25 publications

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“…As kanamycin tolerance was not altered in an E. coli/C. albicans biofilm compared to an axenic E. coli biofilm, this indicates that the observed increased tolerance of E. coli in the presence of C. albicans is drug dependent, which is in line with recently published research of Kart and colleagues, who show that the effect of a species on the susceptibility of another species depends on the disinfectant used (43). The increased ofloxacin tolerance of E. coli in the presence of C. albicans is less pronounced under planktonic conditions, suggesting that biofilm-specific characteristics, such as the extracellular matrix, might contribute significantly to this observed increased ofloxacin tolerance.…”

Section: Figsupporting

confidence: 77%

Fungal β-1,3-Glucan Increases Ofloxacin Tolerance of Escherichia coli in a Polymicrobial E. coli/Candida albicans Biofilm

Brucker

Tan

Vints

et al. 2015

Antimicrob Agents Chemother

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cIn the past, biofilm-related research has focused mainly on axenic biofilms. However, in nature, biofilms are often composed of multiple species, and the resulting polymicrobial interactions influence industrially and clinically relevant outcomes such as performance and drug resistance. In this study, we show that Escherichia coli does not affect Candida albicans tolerance to amphotericin or caspofungin in an E. coli/C. albicans biofilm. In contrast, ofloxacin tolerance of E. coli is significantly increased in a polymicrobial E. coli/C. albicans biofilm compared to its tolerance in an axenic E. coli biofilm. The increased ofloxacin tolerance of E. coli is mainly biofilm specific, as ofloxacin tolerance of E. coli is less pronounced in polymicrobial E. coli/C. albicans planktonic cultures. Moreover, we found that ofloxacin tolerance of E. coli decreased significantly when E. coli/C. albicans biofilms were treated with matrix-degrading enzymes such as the ␤-1,3-glucan-degrading enzyme lyticase. In line with a role for ␤-1,3-glucan in mediating ofloxacin tolerance of E. coli in a biofilm, we found that ofloxacin tolerance of E. coli increased even more in E. coli/C. albicans biofilms consisting of a high-␤-1,3-glucan-producing C. albicans mutant. In addition, exogenous addition of laminarin, a polysaccharide composed mainly of poly-␤-1,3-glucan, to an E. coli biofilm also resulted in increased ofloxacin tolerance. All these data indicate that ␤-1,3-glucan from C. albicans increases ofloxacin tolerance of E. coli in an E. coli/C. albicans biofilm. Biofilms are well-structured populations of microbial cells that are attached to a surface and embedded in a self-produced extracellular polymer matrix (1, 2). Such biofilms can be found in natural, industrial, and medical environments and can be employed for a variety of biotechnological applications (3-5). However, these structured communities also have great significance for public health, as biofilm microbial cells exhibit increased tolerance to antimicrobial agents (6). Biofilms consisting of a single pathogenic microorganism have been extensively studied in the past, and multiple processes and various structural elements have previously been implicated in axenic biofilm formation, i.e., biofilms consisting of one microbial species. For the Gram-negative bacterium Escherichia coli, examples include motility, virulence, surface appendages, polysaccharides, toxin-antitoxin modules, quorum sensing, and several stress responses (7,8). In the fungal opportunistic pathogen Candida albicans, morphological transition, quorum sensing, adhesins, and several transcription regulators are implicated in axenic biofilm development (9-12). However, as it is becoming increasingly clear that polymicrobial biofilms are the dominant form in nature, the scientific focus is shifting toward polymicrobial biofilms. Communication, cell wall components, metabolic commensalism, and competition for nutrients and physical resources are emerging as important factors influencing the physiology of ...

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Section: Figsupporting

confidence: 77%

Fungal β-1,3-Glucan Increases Ofloxacin Tolerance of Escherichia coli in a Polymicrobial E. coli/Candida albicans Biofilm

Brucker

Tan

Vints

et al. 2015

Antimicrob Agents Chemother

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“…The inoculum cell numbers were based on preliminary optimization experiments, and led to biofilms with the highest cell numbers (data not shown). BHI was supplemented with 5% (w/v) bovine serum albumin (BSA) (Kart et al, 2014), 0.5% (w/v) mucine type II, and 0.3% (w/v) agar. Mucine and agar were added to mimic the composition of CF sputum and to increase the medium viscosity, respectively.…”

Section: Methodsmentioning

confidence: 99%

Quantification ofPseudomonas aeruginosain multispecies biofilms using PMA-qPCR

Tavernier

Coenye

2015

PeerJ

Self Cite

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Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.

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“…In most of these studies the enhanced tolerance was attributed to 487 protective effects of the species on each other, however, generally without unraveling the mechanism 488 of tolerance. In contrast, a minority of studies did not observe an effect of multispecies conditions on 489 antimicrobial tolerance (Gkana et al, 2017) or did even measure a decrease in tolerance in 490 multispecies conditions (Lindsay et al, 2002;Chorianopoulos et al, 2008;Kart et al, 2014;Yassin et 491 al., 2016;Feldman et al, 2016). 492…”

Section: Direct Effect Of Competitors On Antimicrobial Tolerance 398mentioning

confidence: 99%

Competitive inter-species interactions underlie the increased antimicrobial tolerance in multispecies brewery biofilms

Parijs

Steenackers

2017

Preprint

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16Genetic diversity often enhances the tolerance of microbial communities against antimicrobial 17 treatment. However the sociobiology underlying this antimicrobial tolerance remains largely 18 unexplored. Here we analyze how inter-species interactions can increase antimicrobial tolerance. We 19 apply our approach to 17 industrially-relevant multispecies biofilm models, based on species isolated 20 from 58 contaminating biofilms in three breweries. Sulfathiazole is used as antimicrobial agent 21 because it shows the highest activity out of 22 biofilm inhibitors tested. Our analysis reveals that 22 competitive interactions dominate among species within brewery biofilms. We show that 23 antimicrobial treatment can reduce the level of competition and therefore cause a subset of species 24 to bloom. The result is a lower percentage inhibition of these species and increased tolerance. In 25 addition, we show that the presence of competing species can also directly enhance the inherent 26

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Activity of disinfectants against multispecies biofilms formed byStaphylococcus aureus,Candida albicansandPseudomonas aeruginosa

Cited by 44 publications

References 25 publications

Fungal β-1,3-Glucan Increases Ofloxacin Tolerance of Escherichia coli in a Polymicrobial E. coli/Candida albicans Biofilm

Fungal β-1,3-Glucan Increases Ofloxacin Tolerance of Escherichia coli in a Polymicrobial E. coli/Candida albicans Biofilm

Quantification ofPseudomonas aeruginosain multispecies biofilms using PMA-qPCR

Competitive inter-species interactions underlie the increased antimicrobial tolerance in multispecies brewery biofilms

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