Activity of cholesterol oxidase immobilized on Layered Double Hydroxide nanomaterials for biosensor application: Acacia salicina scavenging power of hypercholesterolemia therapy

Temani, Marwa; Baccar, Z. M.; Mansour, Hédi Ben

doi:10.1016/j.mee.2014.07.009

Cited by 9 publications

(3 citation statements)

References 34 publications

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“…In addition to conventional nanomatrices, various novel materials have been explored for their capacity to immobilize cholesterol oxidase. Successful immobilization of cholesterol oxidase has been carried out on Mg 2 AlCO 3 Hydrotalcite Layered Double Hydroxide nanomaterials for biosensor applications [103]. This group used extracts of Acacia salicina leaves in order to scavenge the free radicals such as H 2 O 2 liberated during the enzymatic reaction.…”

Section: Immobilization On Other Nanoparticlesmentioning

confidence: 99%

Immobilization of Cholesterol Oxidase: An Overview

Ghosh¹,

Ahmad²,

Khare³

2018

TOBIOTJ

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Background: Cholesterol oxidases are bacterial oxidases widely used commercially for their application in the detection of cholesterol in blood serum, clinical or food samples. Additionally, these enzymes find potential applications as an insecticide, synthesis of anti-fungal antibiotics and a biocatalyst to transform a number of sterol and non-sterol compounds. However, the soluble form of cholesterol oxidases are found to be less stable when applied at higher temperatures, broader pH range, and incur higher costs. These disadvantages can be overcome by immobilization on carrier matrices. Methods: This review focuses on the immobilization of cholesterol oxidases on various macro/micro matrices as well as nanoparticles and their potential applications. Selection of appropriate support matrix in enzyme immobilization is of extreme importance. Recently, nanomaterials have been used as a matrix for immobilization of enzyme due to their large surface area and small size. The bio-compatible length scales and surface chemistry of nanoparticles provide reusability, stability and enhanced performance characteristics for the enzyme-nanoconjugates. Conclusion: In this review, immobilization of cholesterol oxidase on nanomaterials and other matrices are discussed. Immobilization on nanomatrices has been observed to increase the stability and activity of enzymes. This enhances the applicability of cholesterol oxidases for various industrial and clinical applications such as in biosensors.

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Section: Immobilization On Other Nanoparticlesmentioning

confidence: 99%

Immobilization of Cholesterol Oxidase: An Overview

Ghosh¹,

Ahmad²,

Khare³

2018

TOBIOTJ

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“…In fact, due to their particular structure (consisting of positively charged cation layers with charge balancing anions and water molecules inserted between these layers), inorganic LDH matrixes have presented specific properties, such as their high ion exchange capacity, their encapsulation ability and their biocompatibility [3]. Many applications have been proposed, based on the immobilization of different enzymes into LDH like urease [4], cholesterol oxidase [5] and lactate dehydrogenase [6].…”

Section: Introductionmentioning

confidence: 99%

Hydrocalumite Thin Films for Polyphenol Biosensor Elaboration

Soussou

Gammoudi

Kalboussi

et al. 2017

IEEE Trans.on Nanobioscience

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Hybrid thin films based on Hydrocalumite (CaAlCl layered double hydroxide LDH) and tyrosinaseenzyme have been used for the elaboration of a high sensitive amperometric biosensor detecting polyphenols extracted from green tea. Structural properties of LDH nanomaterials were characterized by X-ray powder diffraction and Infra-Red spectroscopy, confirming its crystalline phase and chemical composition. CaAlCl-LDHs-thin films were deposited by spin-coating, and studied by atomic force microscopy to obtain information about the surface morphology of this host matrix before and after enzyme's immobilization. Electrochemical study using cyclic voltammetry and chronoamperometry shows good performances of the built-in biosensor with a high sensitivity for polyphenols concentrations ranging from 24 pM to and a limit of detection of 1.2 pM.

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“…LDH nanomaterial was considered as good host matrix for antibody immobilization thanks to its bidirectional structure, high opening and anionic capacity of exchange and its large surface area [12]. Thanks to their attractive properties they are considered as suitable to promote the best conformation for metabolic reactions [13] or protein/protein interactions [14]. LDHs are known as an important class of anionic lamellar clays and could be represented by the general formula , where are divalent cations , MIII are trivalent cations , and are the anions intercalated into the interlayer with water molecules to compensate for positive charge of the foliar layer of LDHs structure [15].…”

mentioning

confidence: 99%

Development of an Immunosensor Based on Layered Double Hydroxides for MMR Cancer Biomarker Detection

Hammami¹,

Soussou²,

Idoudi³

et al. 2015

IEEE Trans.on Nanobioscience

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Abstract-As a potential biomarker for the investigation of cancer inflammatory profiles, macrophage mannose receptor (MMR, CD206) is herein selected to develop an immunosensor based on layered double hydroxide (LDH). Like an endocyte C-type lectin receptor, MMR plays an important role in immune homeostasis by scavenging unwanted mannose glycoproteins. It attracts a progressive attention thanks to its particularly high expression within the tumor microenvironment. There is a great of interest to develop an immunosensor based on an antibody specific to MMR for detection of stroma versus tumor cells. In this work, we studied the feasibility of high sensitive MMR cancer Screen Printed Electrode (SPE) immunosensor. Working electrode of commercialized SPE was modified by immobilization of specific antibody (anti-MMR) into thin layer of LDH nanomaterials. Structural, morphological, and surface properties of LDHs were studied by X-Ray diffraction, atomic force microscopy and Infrared spectroscopy in ATR. Cyclic Voltammetry technique was used to study interaction between the human recombinant MMR protein (rHu-MMR, NSO derived) and an immobilized antibody into developed immunosensor. High specific response of (with a correlation coefficient of ) were obtained in linear range of 0.05 ng/mL to 10.0 ng/mL of specific recombinant antigen. The limit of detection (LOD) was less than 15.0 pg/mL. From these attractive results, the feasibility of an electrochemical immunosensor for cancer was proved. Additional experiments to study stability and reproducibility the immunosensor should be completed in perspective to use these anti-MMR based immunosensors for sensing human MMR in patient biopsies and sera.Index Terms-Immunosensor, layered double hydroxide, macrophage mannose receptor, nanomaterials.

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Activity of cholesterol oxidase immobilized on Layered Double Hydroxide nanomaterials for biosensor application: Acacia salicina scavenging power of hypercholesterolemia therapy

Cited by 9 publications

References 34 publications

Immobilization of Cholesterol Oxidase: An Overview

Immobilization of Cholesterol Oxidase: An Overview

Hydrocalumite Thin Films for Polyphenol Biosensor Elaboration

Development of an Immunosensor Based on Layered Double Hydroxides for MMR Cancer Biomarker Detection

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