Activity of bone morphogenetic protein-7 after treatment at various temperatures: Freezing vs. pasteurization vs. allograft

Takata, Munehisa; Sugimoto, Naotoshi; Yamamoto, Norio; Shirai, Toshiharu; Hayashi, Katsuhiro; Nishida, Hiroyuki; Tanzawa, Yoshikazu; Kimura, Hiroaki; Miwa, Shinji; Takeuchi, Akihiko; Tsuchiya, Hiroyuki

doi:10.1016/j.cryobiol.2011.09.001

Cited by 67 publications

(54 citation statements)

References 24 publications

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“…These are also seen, although less frequently, with frozen autografts; we experienced only three fractures and two non-unions in the present series. This is because autogenous proteins, growth factors, and cytokines are preserved in the frozen bone [22], which could induce good osteoinduction and osteoconduction to achieve satisfactory bone union and revitalization without eliciting an immune reaction. In addition, there is a perfect fit enabling easy and rigid fixation using simple osteosynthesis techniques and avoiding mal-or non-union.…”

Section: Discussionmentioning

confidence: 99%

“…In bone retrieved more than a year after implantation, osteocytes and osteoblasts were observed in broad portions of the frozen autograft, and the cortical host-graft junction showed incorporation along with continuity of bone trabeculae, indicating the onset of osteogenesis in the frozen autograft at an early stage. It may be the case that, as mentioned above, frozen bone preserves bone morphogenetic protein and its osteoinductive ability better than hyperthermic treatments such as autoclaving and pasteurization [22]. Furthermore, if the pedicle-freezing procedure method, which is a specific procedure of frozen autografts and distinguishes from other recycled bone, is possible, the number of osteotomy sites can be decreased, allowing for a faster bone union [25].…”

Section: Discussionmentioning

confidence: 99%

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Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen

Higuchi

Yamamoto

Nishida

et al. 2017

International Orthopaedics (SICOT)

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Purpose To preserve the joint structure in order to maintain good limb function in patients with osteosarcoma, we perform epiphyseal or metaphyseal osteotomy and reconstruction using frozen autografts that contain a tumour treated with liquid nitrogen. There are two methods of using liquid nitrogen-treated autografts: the free-freezing method and the pedicle-freezing method. The purpose of this study was to evaluate the results of intentional joint-preserving reconstruction using the free-freezing method and the pedicle-freezing method in patients with osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen

Higuchi

Yamamoto

Nishida

et al. 2017

International Orthopaedics (SICOT)

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“…Takata et al reported that bone morphogenetic activity was better preserved in frozen autografts treated with liquid nitrogen than in those treated by autoclaving or pasteurization [24].…”

Section: Discussionmentioning

confidence: 99%

“…Intercalary reconstruction with the use of a frozen autograft has many advantages; it is a relatively simple and low cost technique, allows a precise fit between the graft and host bone, preserves osteoinduction and osteoconduction [24], averts graft rejection, avoids the transmission of infectious diseases, preserves the bone stock and biomechanical strength, allows easy attachment of ligaments and tendons to bone, prevents harmful denaturation of substances and provides early revitalization with possible cryoimmunological effects [17].…”

Section: Discussionmentioning

confidence: 99%

Intercalary frozen autograft for reconstruction of malignant bone and soft tissue tumours

Zekry

Yamamoto

Hayashi

et al. 2017

International Orthopaedics (SICOT)

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Purpose In 1999, we developed a technique using frozen autografts-tumour-containing bone treated with liquid nitrogen-for the reconstruction of malignant bone tumours. The purpose of this study was to evaluate the functional and oncological outcomes of frozen autografts for intercalary reconstruction of malignant bones and soft tissue tumours. Methods This retrospective study was designed to assess 34 patients of mean age 35 (range, 6-79) years. The mean followup period was 62 (24-214) months. The median length of the frozen autografts was 138.4 ± 60.39 (50-290) mm. Results Postsurgically, 20 patients remained disease-free, seven patients survived with no evidence of disease, five patients were alive with disease, and two patients died of disease. The five-and ten-year survival rates of the frozen autografts were 91.2% and the mean International Society of Limb Salvage score was 90%. Complete bony union was achieved in 97% of the patients. There were five cases of nonunion, six cases of fracture, two cases of deep infection and four cases of local recurrence. Conclusion Utilizing intercalary frozen autografts for patients with a nonosteolytic primary or secondary bone tumour without involvement of the subchondral bone is a good alternative treatment, because it is a straightforward biological technique and can provide excellent limb function.

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“…That includes simple procedure without any specific equipment and complicated temperature control; strength of treated bone and superior osteo-conductive and osteo-inductive capacity with spared matrix proteins and enzymes; best compatibility of size and form and attachment of soft tissues such as ligaments and tendons [2,3,11]. These advantages could lead to more preferable bone regeneration and limb functions.…”

Section: Characteristics Of Liquid Nitrogen-treated Bonementioning

confidence: 99%

Biological Reconstruction Using Liquid Nitrogen-Treated Tumor Bearing Bone

Yamamoto¹,

Tsuchiya²

2017

Osteosarcoma - Biology, Behavior and Mechanisms

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In general, a tumor prosthesis is used for reconstruction after removal of osteosarcoma. However, the durability of artificial materials becomes a problem in the long term, and many patients inevitably undergo revision due to loose or damaged prostheses. Moreover, preservation of articular surface is the key to maintain better limb function for long duration. Reconstruction of affected limbs using biological materials has been sought to overcome the aforementioned problems. In some countries, it is significantly difficult to obtain allograft as a biological reconstruction material because of socio-religious or cost problem. Thus, a biological reconstruction method has been developed in which the patient's affected bone is treated and used for reconstruction. Especially in recycling treatment for affected autologous bone, liquid nitrogen treatment has several favorable characteristics. There is optimal morphological fit because the treated bone itself is one's own, and bone strength is maintained after treatment. Satisfactory bone union and bone regeneration are expected to be achieved due to good osteoconduction and osteoinduction because proteins and enzymes are preserved in the bone.

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Activity of bone morphogenetic protein-7 after treatment at various temperatures: Freezing vs. pasteurization vs. allograft

Cited by 67 publications

References 24 publications

Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen

Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen

Intercalary frozen autograft for reconstruction of malignant bone and soft tissue tumours

Biological Reconstruction Using Liquid Nitrogen-Treated Tumor Bearing Bone

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