Activity of Antioxidative Enzymes in Erythrocytes after a Single Dose Administration of Doxorubicin in Rats Pretreated with Fullerenol C₆₀(OH)₂₄

Abstract

In earlier in vitro investigations, fullerenol was shown to have a strong antioxidative capability. The present study examined the role of fullerenol as a potential antioxidative protector for doxorubicin-induced oxidative stress in the blood of rats through an investigation of the activity of glutathione-dependent enzymes (glutathione-S-transferase and glutathione peroxidase). It also assessed the influence of fullerenol on the number of blood cells (leukocytes and erythrocytes) as well as on the content of hemoglobin after a single dose administration of doxorubicin. Experiments were performed on six groups of adult male Wistar rats, each group containing eight individuals. Doxorubicin was administrated i.v. (tail vein) in a single dose of 10 mg/kg. Fullerenol C(60)(OH)(24) was administrated to the treated animals i.p. (in doses 50, 100, 200 mg/kg) 30 min before the dosing with doxorubicin. The control group animals were given saline (1 ml/kg; i.p.). One group of animals was treated only with fullerenol (100 mg/kg i.p.). The animals were sacrificed 2 and 14 days after the treatment. Each experiment was repeated twice. The results may indicate that fullerenol induces a decrease in the antioxidative capacity of erythrocytes in oxidative stress conditions, whereas, without doxorubicin, the application of fullerenol did not induce any changes in the enzyme activity of erythrocytes. The results of GST activity might indicate that 50 mg/kg are not sufficient to protect from doxorubicin toxicity, while 200 mg/kg might be toxic for animals, judging from the increase in GST activity.

Effects of Single and Combined Losartan and Tempol Treatments on Oxidative Stress, Kidney Structure and Function in Spontaneously Hypertensive Rats with Early Course of Proteinuric Nephropathy

“…injection of ADR (20 mg/kg) in Sprague–Dawley rats caused significant enhancement of protein oxidation in the kidney [22]. Other studies found significant increases of lipid peroxidation in plasma, but no changes in antioxidant activities of SOD, CAT and GSH-Px in erythrocytes [23,24]. These results are in agreement with finding from the present study.…”

Antioxidative Effect of Rice Bran Oil and Medium Chain Fatty Acid Rich Rice Bran Oil in Arsenite Induced Oxidative Stress in Rats

“…The activities of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase form the first line of defense against reactive oxygen species 30 . With the administration of sodium arsenite there was a decrease in the activities of the enzymes which proved the generation of oxidative stress.…”

Markers of Oxidative Stress in Erythrocytes and Plasma During Aging in Humans

“…5 ). 92 SOD catalyzes the dismutation of superoxide radical (•O 2 ) to hydrogen peroxide (H 2 O 2 ). Although H 2 O 2 is not a radical, it is rapidly converted by fenton reaction into •OH radical which is very reactive.…”