Activity of Antioxidant Enzymes in Response to Cadmium in Arabidopsis thaliana

Saffar, A.; Najjar, Mohammad Bagher Bagherieh; Mianabadi, Manijeh

doi:10.3923/jbs.2009.44.50

Cited by 30 publications

(9 citation statements)

References 35 publications

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“…Plants have their own enzymatic antioxidant defense system of a specific nature, including POD, SOD, and catalase . This defense system scavenges ROS to protect cells against oxidative damage . MDA, the main effector of lipid peroxidation, is synthesized during germination and has an adverse effect on seed germination .…”

Section: Resultsmentioning

confidence: 99%

Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

Chang

Deng²,

Zhang

et al. 2018

J. Agric. Food Chem.

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Gnetum parvifolium is a rich source of materials for traditional medicines, food, and oil, but little is known about the mechanism underlying its seed dormancy and germination. In this study, we analyzed the proteome-level changes in its seeds during germination using isobaric tags for relative and absolute quantitation. In total, 1,040 differentially expressed proteins were identified, and cluster analysis revealed the distinct time points during which signal transduction and oxidation-reduction activity changed. Gene Ontology analysis showed that "carbohydrate metabolic process" and "response to oxidative stress" were the main enriched terms. Proteins associated with starch degradation and antioxidant enzymes were important for dormancy-release, while proteins associated with energy metabolism and protein synthesis were up-regulated during germination. Moreover, protein-interaction networks were mainly associated with heat-shock proteins. Furthermore, in accord with changes in the energy metabolism- and antioxidant-related proteins, indole-3-acetic acid, Peroxidase, and soluble sugar content increased, and the starch content decreased in almost all six stages of dormancy and germination analyzed (S1-S6). The activity of superoxide dismutase, abscisic acid, and malondialdehyde content increased in the dormancy stages (S1-S3) and then decreased in the germination stages (S4-S6). Our results provide new insights into G. parvifolium seed dormancy and germination at the proteome and physiological levels, with implications for improving seed propagation.

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Section: Resultsmentioning

confidence: 99%

Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

Chang

Deng²,

Zhang

et al. 2018

J. Agric. Food Chem.

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“…Similar our study, Chinmayee et al (2014) demonstrated that, PPO activity in Jatropha curcas L. increased in all plant parts under chromium, cadmium and lead stress. Also, Saffar et al (2009) reported that in Arabidopsis thaliana, PPO activity might be the result of extended heavy metal stress. PPO catalyzes the formation of highly active Quinone that reacts with amino or sulfhydryl groups in proteins or enzymes.…”

Section: Discussionmentioning

confidence: 99%

PROTECTIVE EFFECTS OF EXOGENOUS NITRIC OXIDE AGAINST LEAD TOXICITY IN LEMON BALM (Melissa officinalis L.)

Jafarnezhad-Moziraji¹

2017

Appl. Ecol. Env. Res.

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This research investigated the effects of exogenous sodium nitroprusside (SNP) supplementation as nitric oxide (NO) on alleviating Pb-induced oxidative damage in lemon balm (Melissa officinalis L.) plants. Pb (100, 300 and 500 μM) alone and in combination with SNP (100 and 200 μM) were given to hydroponically grown Melissa officinalis L. plants. The findings suggested that in Pbtreated plants, height, total dry weight, and chlorophyll content of leaves markedly decreased while application of 100 μM SNP alleviated the inhibitory effect of Pb on plant growth and chlorophyll content. Pb exposure caused oxidative stress by elevating lipid hydroperoxide contents of the seedlings. Application of 100 μM SNP counteracted Pb toxicity by reducing lipid hydroperoxide contents of Pbtreated seedlings. Furthermore, it was found that the activities of antioxidant enzymes such as polyphenol oxidase, catalase, guaiacol peroxidase, and ascorbate peroxidase were increased in Pb-treated plants. The use of NO especially at low concentrations reversed Pb-induced negative effects whereas high concentrations of NO had no obvious alleviating effect on Pb toxicity in Melissa officinalis L. On the other hand, application of 100 μM SNP could function as a defense mechanism of the plant against Pb toxicity and mitigate Pb stress.

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“…Catalase (EC 1.11.1.6) activity was estimated by the method followed by Pereira et al 2002. To a mixture of 50 mM potassium phosphate buffer (pH 7.0) and 0.036% of H 2 O 2, 0.1 ml of enzyme extract was added and the decrease in absorbance was recorded at 240 nm and the rate of enzyme activity was expressed as U mg protein −1 .…”

Section: Methodsmentioning

confidence: 99%

Exogenously applied ABA induced changes in physio-biochemical attributes in selected rice varieties under osmotic stress

Dash

Geda

Sahoo

et al. 2020

Preprint

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Activity of Antioxidant Enzymes in Response to Cadmium in Arabidopsis thaliana

Cited by 30 publications

References 35 publications

Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

PROTECTIVE EFFECTS OF EXOGENOUS NITRIC OXIDE AGAINST LEAD TOXICITY IN LEMON BALM (Melissa officinalis L.)

Exogenously applied ABA induced changes in physio-biochemical attributes in selected rice varieties under osmotic stress

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