Activity of antioxidant enzymes and concentration of lipid peroxidation products in selected tissues of mice of different ages, both healthy and melanoma-bearing

Woźniak, Alina; Drewa, Gerard; Woźniak, Bartosz; Schachtschabel, D.O.

doi:10.1007/s00391-004-0229-y

Cited by 35 publications

(26 citation statements)

References 19 publications

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“…There were no statistical differences in the total enzyme activity in the group of older and younger females which may suggest no dependence on age in livers of these animals, which is also confirmed by other authors (Tian et al 1998, Sverko et al 2004). There are, however, others who observed increased SOD activity during ageing in mice (Wozniak et al 2004).…”

Section: Discussionmentioning

confidence: 91%

“…Meng et al (2007) found a significant decrease in SOD activity in the kidney, brain, and heart during aging in rats as well as a higher activity of CuZn-SOD than Mn-SOD in older compared to younger animals. Wozniak et al (2004) observed doi: 10.17221/8406-CJAS an increase in enzymatic activity in older animals, but also a decrease in erythrocytes with age. No effect of age on SOD activity was noticed in the mouse brain (Hamilton et al 2001), in the liver of mice (Sverko et al 2004) as well as in the rat muscles (Chen et al 2008).…”

Section: Introductionmentioning

confidence: 93%

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Age and sex-related changes in superoxide dismutase activity in bovine tissues

Giergiel¹,

Kankofer²

2015

CZECH J ANIM SCI

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ABSTRACT:The influence of age, gender, and type of tissue on superoxide dismutase (SOD) activity in bovine organs and tissues was investigated. The investigated material consisted of fragments of tissues and organs (liver, heart, lung, kidney, skeletal muscles, and diaphragm) from healthy cows (n = 15), bulls (n = 15), and female calves (n = 12) collected immediately after slaughter at the slaughterhouse. The total SOD activity was measured in tissue and organ homogenates by spectrophotometric method. PAGE electrophoresis and Western blotting technique with specific anti-SOD antibodies as well as zymography confirmed the presence of enzymatic protein and SOD isoenzymes in examined tissues. The study revealed significant differences in SOD activity between organs and tissues, which might be connected with different metabolic rate. Age-related changes were also observed. SOD activity was twice higher in kidneys, lungs, heart of calves compared to other groups of animals. It was also noticed that SOD activity was higher in younger animals and decreased with ageing among the group of bulls. Gender-related differences were observed in lungs and diaphragm. It was concluded that SOD activity depends on sex steroids, which is reflected in age and gender discrepancies, as well as metabolic rate of tissues.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 93%

Age and sex-related changes in superoxide dismutase activity in bovine tissues

Giergiel¹,

Kankofer²

2015

CZECH J ANIM SCI

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“…SOD, CAT and GPx are the main antioxidant enzymes of the biological system (Wozniak et al, 2004;Slavic et al, 2006;Lestaevel et al, 2009). CAT can convert hydrogen peroxide to molecular oxygen and water.…”

Section: Discussionmentioning

confidence: 99%

Responses of growth performance and tryptophan metabolism to oxidative stress induced by diquat in weaned pigs

Mao

et al. 2012

Animal

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During many pathological conditions, the tryptophan concentration in blood may be reduced. However, the effects of oxidative stress on tryptophan metabolism remain unknown. In this study, we investigated the effects of oxidative stress on growth performance and tryptophan metabolism in weaned pigs. A total of 24 weaned pigs were assigned to one of three treatments that included pigs fed ad libitum (control), pigs challenged with diquat at a dose of 10 mg/kg BW and fed ad libitum (oxidative stress) or pigs pair-fed to receive the same amount of feed as the diquat-challenged pigs. The trial lasted for 7 days. The growth performance and activities of antioxidant enzymes were declined in diquat-challenged pigs. The diquat challenge decreased the tryptophan concentration in serum and the 5-hydroxytryptamine concentration in the hypothalamus, and increased large neutral amino acids, kynurenine (Kyn) and malondialdehyde in serum. The 544-bp porcine partial mRNA sequence of the tryptophan 2,3-dioxygenase (TDO) gene was obtained according to the conserved region in the human gene sequence. In addition, the oxidative stress induced by the diquat challenge stimulated TDO-relative mRNA abundance in the liver and g-glutamyl transpeptidase activity in intestinal mucosa, but did not affect the mRNA levels of Na 1 -neutral amino acid transporter B0. These results suggested that oxidative stress induced by diquat depressed growth performance and increased metabolism of tryptophan via Kyn pathway that upregulated TDO mRNA expression in weaned pigs.

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“…For instance, HO-1 has been reported to increase in brain and liver tissues with aging (81,82), whereas SOD and CAT activities were increased in the brain, and CAT and GPx were elevated in the liver of aged mice (83). Moreover, SOD, CAT, and GPx activities increase in different regions of the aged C57BL/6N brain (84).…”

Section: Discussionmentioning

confidence: 95%

“…Phase II enzymes play an important role during aging, with data from various laboratories showing age-related changes in phase II enzyme expression, with some specificity with respect to tissue type, organelle, animal strain, and sex (81)(82)(83)(84)(85)(86)(87). For instance, HO-1 has been reported to increase in brain and liver tissues with aging (81,82), whereas SOD and CAT activities were increased in the brain, and CAT and GPx were elevated in the liver of aged mice (83).…”

Section: Discussionmentioning

confidence: 99%

The Role of Phase II Antioxidant Enzymes in Protecting Memory T Cells from Spontaneous Apoptosis in Young and Old Mice

Kim

Nel

2005

The Journal of Immunology

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Aging is associated with a functional decline and change in the phenotypic distribution of T cell subsets. The free radical theory of aging is widely promoted as the mechanistic basis for cellular senescence, including the immune system. Although the exact molecular explanation for the role of oxidative stress in cellular senescence is unclear, there is a connection to altered mitochondrial function, both as a contributor and as a target of oxidative stress. In this study we demonstrate that splenic T lymphocytes from old C57BL/6 mice exhibit a significant decline in mitochondrial membrane potential (Δψm). However, despite this change, there is a lower rate of withdrawal apoptosis in the memory CD4+ and CD8+ T cells. To explain the survival of these long-lived cells against a background of increased oxidative stress, we demonstrate increased glutathione production and phase II enzyme expression, which combine to protect memory T cells against oxidative stress, mitochondrial dysfunction, and cell death. The accumulation of memory T cells with aging explains higher phase II enzyme expression in CD4+ and CD8+ T cells from old mice. Compared with wild-type mice, mice lacking the expression of NF-E2-related factor-2, the transcription factor that regulates phase II enzyme expression, had a significantly enhanced rate of apoptosis in the presence of an oxidative stress stimulus. NF-E2-related factor-2-deficient T cells exhibit a bigger decline in Δψm and increased reactive oxygen species production than cells from wild-type animals. Taken together, we suggest that phase II enzyme expression and the accompanying increase in intracellular thiol levels protect memory T cells from mitochondrial dysfunction and spontaneous apoptosis.

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Activity of antioxidant enzymes and concentration of lipid peroxidation products in selected tissues of mice of different ages, both healthy and melanoma-bearing

Cited by 35 publications

References 19 publications

Age and sex-related changes in superoxide dismutase activity in bovine tissues

Age and sex-related changes in superoxide dismutase activity in bovine tissues

Responses of growth performance and tryptophan metabolism to oxidative stress induced by diquat in weaned pigs

The Role of Phase II Antioxidant Enzymes in Protecting Memory T Cells from Spontaneous Apoptosis in Young and Old Mice

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