Activity of amine oxide against biofilms of Streptococcus mutans: a potential biocide for oral care formulations

Fraud, Sébastien; Maillard, Jean‐Yves; Kaminski, Michael A.; Hanlon, G. W.

doi:10.1093/jac/dki325

Cited by 21 publications

(30 citation statements)

References 31 publications

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“…The preparation of S. mutans biofilms S. mutans biofilms [UA140::φ (mutAp-mrfp1)] were formed on saliva-coated hydroxyapatite discs; to grow the S. mutans biofilms, a technique described by Fraud et al was modified [21] S. mutans was cultured on sintered circular ceramic HA disks (9.8 mm in diameter×1.5 mm in height; Clarkson Chromatography Products, South Williamsport, PA, USA) that had been incubated with saliva from rats that had not been immunized. The saliva to cultivate the biofilms was pre-filtered through a 5-μm and then a 0.45-μm filter (Fisher Scientific, Loughborough, UK).…”

Section: The Adherence Inhibition Assaymentioning

confidence: 99%

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Huang

Liu

et al. 2012

Acta Pharmacol Sin

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Aim: To investigate the effects of anti-caries DNA vaccine-induced salivary secretory immunoglobulin A (S-IgA) antibodies on Streptococcus mutans (S. mutans) adherence and biofilms formation in vitro. Methods: Adult female Wistar rats were intranasally immunized with the anti-caries DNA vaccine pGJA-P/VAX. Their saliva samples were collected at different times after the immunization, and S-IgA antibody level in the saliva and its inhibition on S. mutans adherence were examined. The effects of S-IgA in the saliva with the strongest inhibitory effects were examined at 3 different stages, ie acquired pellicles, biofilm formation and production of mature biofilms. The number of viable bacteria and depth of the biofilm at 16 h in each stage were determined using counting colony forming units and using a confocal laser scanning microscopy (CLSM). The participation of S-IgA in acquired pellicles and its aggregation with S. mutans were also observed under CLSM. Results: The S-IgA titer in saliva reached its peak and exhibited the strongest inhibition on S. mutans adhesion at 10 weeks after the immunization. The colonies and depth of the biofilm in the saliva-pretreated group were 41.79% and 41.02%, respectively, less than the control group. The colonies and depth of the biofilm in the co-culture group were 27.4% and 22.81% less than the control group. The assembly of S. mutans and S-IgA was observed under CLSM after co-cultivation. In the mature-stage biofilm, no differences were observed between the different groups.Conclusion: These results demonstrate that the anti-caries DNA vaccine induces the production of specific S-IgA antibodies that may prevent dental caries by inhibiting the initial adherence of S. mutans onto tooth surfaces, thereby reducing the accumulation of S. mutans on the acquired pellicles.

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Section: The Adherence Inhibition Assaymentioning

confidence: 99%

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Huang

Liu

et al. 2012

Acta Pharmacol Sin

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“…A number of authors suggest that biofilm formed over an extensive period of time has increased resistance to antimicrobial substances (20)(21)(22)(23)(24)(25)(26), while others have reported that the age of the biofilm does not enhance resistance to disinfectants (27). Increased extrapolymeric substances and biofilm thickness over time are reported by some to enhance resistance to disinfectant agents (18,28).…”

mentioning

confidence: 99%

Commonly Used Disinfectants Fail To Eradicate Salmonella enterica Biofilms from Food Contact Surface Materials

Corcoran

Morris

Lappe

et al. 2014

Appl Environ Microbiol

168

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Salmonellosis is the second most common cause of food-borne illness worldwide. Contamination of surfaces in food processing environments may result in biofilm formation with a risk of food contamination. Effective decontamination of biofilm-contaminated surfaces is challenging. Using the CDC biofilm reactor, the activities of sodium hypochlorite, sodium hydroxide, and benzalkonium chloride were examined against an early (48-h) and relatively mature (168-h) Salmonella biofilm. All 3 agents result in reduction in viable counts of Salmonella; however, only sodium hydroxide resulted in eradication of the early biofilm. None of the agents achieved eradication of mature biofilm, even at the 90-min contact time. Studies of activity of chemical disinfection against biofilm should include assessment of activity against mature biofilm. The difficulty of eradication of established Salmonella biofilm serves to emphasize the priority of preventing access of Salmonella to postcook areas of food production facilities.

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“…The bacteriostatic and bactericidal activity of AO on several microorganisms has recently been reported (10,11). AO has been shown to possess anti-bacterial function, and to reduce oral streptococcal plaque and biofilm formation.…”

Section: Chapter 3 Research Objectivesmentioning

confidence: 99%

“…While caustic to fungal and bacterial pathogens, sodium hypochlorite is also very abusive to intraoral tissues and may affect denture base chromaticity when not rinsed completely following application. Fraud, et al has stated in previous projects that low concentrations of AO are safe for intraoral tissues, and even safe for intraoral use and application (10,11).…”

Section: Effect Of Ao On Adherence Of C Albicans To Pmma Discsmentioning

confidence: 99%

“…These surfactants are not only very mild (making them useful for personal care and household cleaning products), but also have a disinfecting/sanitizing property (imparted by the cationic nature of the molecule) (9) that has recently found an oral application. In a study by Fraud, et al, AO was found to be an excellent biocide for eliminating Streptococcus mutans biofilms from hydroxyapetite discs (10,11). No research has been done on the efficacy of AO as a biocide to eliminate C. albicans from denture acrylic surfaces.…”

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The Influence of Biofilm on the Antifungal Activity of Amine Oxide

Hart¹

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Activity of amine oxide against biofilms of Streptococcus mutans: a potential biocide for oral care formulations

Abstract: Amine oxide showed high potential for controlling early biofilms caused by periodontal bacteria. Further investigations should be carried out, particularly on the potential toxicity of amine oxide and its efficacy in complex formulations for oral care products.

Cited by 21 publications

References 31 publications

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Commonly Used Disinfectants Fail To Eradicate Salmonella enterica Biofilms from Food Contact Surface Materials

The Influence of Biofilm on the Antifungal Activity of Amine Oxide

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