Activity of 3′-Hydroxychalcone Against
            <i>Cryptococcus Gattii</i>
            and Toxicity, and Efficacy in Alternative Animal Models

Palanco, Ana Cerrejón; Singulani, Júnya de Lacorte; Costa-Orlandi, Caroline Barcelos; Gullo, Fernanda Patrícia; Lourencetti, Natália Manuela Strohmayer; Gomes, Paulo César; Ayusso, Gabriela Miranda; Dutra, Luiz Antônio; Bolzani, Vanderlan da Silva; Regasini, Luís Octávio; Mendes-Giannini, María José Soares; Fusco‐Almeida, Ana Marisa

doi:10.2217/fmb-2017-0062

Cited by 26 publications

(21 citation statements)

References 41 publications

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“…, Cryptococcus spp. [32,33,[45][46][47][48][49][50][51], Aspergillus spp. [13,29,35,[52][53][54][55][56][57][58][59][60][61], Mucorales [62][63][64], Madurella mycetomatis [65][66][67], and other fungal agents [68][69][70].…”

Section: Evaluation Of Antifungal Efficacy In Galleria Mellonellamentioning

confidence: 99%

“…The potential use of G. mellonella for evaluation of treatment efficacy has also been highlighted in several studies that focused on the development of new molecules with antifungal activities against Cryptococcus spp. [32,46,[48][49][50].…”

Section: Cryptococcus Sppmentioning

confidence: 99%

“…Sometimes, in vitro activity is not translated to in vivo efficacy in G. mellonella. For example, Palanco et al [48] tested the efficacy of 3 -hydroxychalcone. Chalcones, which are abundant in plants, have numerous pharmacological activities including antifungal action [80].…”

Section: Cryptococcus Sppmentioning

confidence: 99%

“…In vitro, 3 hydroxychalone was fungicidal against C. gattii both in planktonic and biofilm form but did not decrease the mortality or the fungal burden of infected larvae. This lack of correlation between in vitro activity and in vivo efficacy could possibly be explained by the high hydrophobicity of the compound responsible for a limited distribution in tissues [48].…”

Section: Cryptococcus Sppmentioning

confidence: 99%

See 3 more Smart Citations

Galleria mellonella for the Evaluation of Antifungal Efficacy against Medically Important Fungi, a Narrative Review

et al. 2020

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The treatment of invasive fungal infections remains challenging and the emergence of new fungal pathogens as well as the development of resistance to the main antifungal drugs highlight the need for novel therapeutic strategies. Although in vitro antifungal susceptibility testing has come of age, the proper evaluation of therapeutic efficacy of current or new antifungals is dependent on the use of animal models. Mammalian models, particularly using rodents, are the cornerstone for evaluation of antifungal efficacy, but are limited by increased costs and ethical considerations. To circumvent these limitations, alternative invertebrate models, such as Galleria mellonella, have been developed. Larvae of G. mellonella have been widely used for testing virulence of fungi and more recently have proven useful for evaluation of antifungal efficacy. This model is suitable for infection by different fungal pathogens including yeasts (Candida, Cryptococcus, Trichosporon) and filamentous fungi (Aspergillus, Mucorales). Antifungal efficacy may be easily estimated by fungal burden or mortality rate in infected and treated larvae. The aim of the present review is to summarize the actual data about the use of G. mellonella for testing the in vivo efficacy of licensed antifungal drugs, new drugs, and combination therapies.

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Section: Evaluation Of Antifungal Efficacy In Galleria Mellonellamentioning

confidence: 99%

Section: Cryptococcus Sppmentioning

confidence: 99%

Section: Cryptococcus Sppmentioning

confidence: 99%

Section: Cryptococcus Sppmentioning

confidence: 99%

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Galleria mellonella for the Evaluation of Antifungal Efficacy against Medically Important Fungi, a Narrative Review

et al. 2020

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“…Among these alternative models, the Galleria mellonella model is gaining popularity because, unlike other non-mammal models, it allows for both, toxicity and antimicrobial activity testing [8][9][10][11][12][13][14]. This is due to the fact that larvae survive at 37°C [15].…”

Section: Introductionmentioning

confidence: 99%

New solvent options for in vivo assays in the Galleria mellonella larvae model

et al. 2019

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Experimentation in mammals is a long and expensive process in which ethical aspects must be considered, which has led the scientific community to develop alternative models such as that of Galleria mellonella. This model is a cost and time effective option to act as a filter in the drug discovery process. The main limitation of this model is the lack of variety in the solvents used to administer compounds, which limits the compounds that can be studied using this model. Five aqueous (DMSO, MeOH, acetic acid, HCl and NaOH) and four non-aqueous (olive oil, isopropyl myristate, benzyl benzoate and ethyl oleate) solvents was assessed to be used as vehicles for toxicity and antimicrobial activity in vivo assays. All the tested solvents were innocuous at the tested concentrations except for NaOH, which can be used at a maximum concentration of 0.5 M. The toxicity of two additional compounds, 5-aminosalicylic acid and DDT, was also assessed. The results obtained allow for the testing of a broader range of compounds using wax moth larvae. This model appears as an alternative to mammal models, by acting as a filter in the drug development process and reducing costs and time invested in new drugs.

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A Galleria Model of Cryptococcus neoformans Infection

Eisenman

2024

Cryptococcus Neoformans

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Activity of 3′-Hydroxychalcone Against Cryptococcus Gattii and Toxicity, and Efficacy in Alternative Animal Models

Abstract: Because of the activity of 3'-hydroxychalcone against C. gattii in vitro, molecular modifications should be made to improve efficacy and to reduce toxicity in vivo. [Formula: see text].

Cited by 26 publications

References 41 publications

Galleria mellonella for the Evaluation of Antifungal Efficacy against Medically Important Fungi, a Narrative Review

Galleria mellonella for the Evaluation of Antifungal Efficacy against Medically Important Fungi, a Narrative Review

New solvent options for in vivo assays in the Galleria mellonella larvae model

A Galleria Model of Cryptococcus neoformans Infection

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