Activity of 2‐α‐L‐fucosyltransferase in human sera and red cell membranes A study of common ABH blood donors, rare ‘Bombay’ and ‘Parabombay’ individuals

Mulet, Céline; Cartron, Jean‐Pierre; Badet, Josette; Salmon, Charles T.

doi:10.1016/0014-5793(77)81060-0

Cited by 45 publications

(21 citation statements)

References 36 publications

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“…The fact that Bh individuals, as shown in previous investigations [18,19,30] like other 'Bombay' and 'Parabombay', are de void of a-(l-2)-fucosyltransferase activity, and the finding that Bh individuals have a normal B transferase activity with a specific ity restricted to H substance as the substrate, leads to the following conclusion concerning the appearance of the 'Parabombay' pheno-I m types.…”

Section: Discussionmentioning

confidence: 77%

“…and Pay., presently under study [18]. The H enzyme was not found either with type 1 or with type 2 acceptor substrates (table I).…”

Section: B and H Gene Products In The Serum And The Rbc From Bh Samplesmentioning

confidence: 88%

“…They have been termed 'Parabombay' [25], Enzymatic studies have shown rather surprisingly, that also in these phenotypes the H enzyme is defective in serum and erythrocytes, but, as expected, that A and/or B enzymes appropriate to the phenotype were found [18,19]. In such cases, A or B …”

Section: Introductionmentioning

confidence: 99%

“…10 ,«1 human B or O red cells washed three times in PBS were incubated were incubated for 72 h at 37 °C and the reaction product was isolated and characterized as previ ously described [18,19]. …”

Section: Assay Of Glycosidasesmentioning

confidence: 99%

“…The 2-a-L-fucosyltransferase, how ever, the direct product of the H gene, is absent or inactive while it is normally found in all individuals with common ABO pheno types [18,21,30], Since the lack of H enzyme prevents the synthesis of the H substance, the precursor of A and B structures [38], A and/or B antigens cannot be present despite the nor mal presence of the A and/or B blood group glycosyltransferases.…”

Section: Introductionmentioning

confidence: 99%

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Probable Biosynthetic Pathway for the Synthesis of the B Antigen from B(h) Variants

Mulet¹,

Cartron²,

Schenkel-Brunner³

et al. 1979

Vox Sanguinis

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Red cells and serum from two B(h) variants (B+H-cells) have been investigated for B and H blood group glycosyltransferases. The H enzyme could not be detected using either type 1 or type 2 chain acceptors. The B enzyme was present in normal amount when 2'-fucosyllactose was used as substrate, neither 6'-fucosyllactose nor 6'-fucosyllactosamine could act as acceptors for the B enzyme. Upon treatment of the B(h) red cells by the B-degrading enzyme from Trichomonas foetus the B antigen was destroyed while H determinants were uncovered (B-H+ cells). The cells thus treated could be further converted into A+H- red cells by the action of the A transferase from human blood group A serum. Previous treatment of the B-H+cells by the H-degrading enzyme from T. foetus, however, led to B-H- erythrocytes and prevented their conversion into A red blood cells by the A enzyme. The results clearly demonstrate that, as found in normal B individuals, the B antigen from B(h) cells is built up from the H precursor and provide additional evidence that H is not a completely silent gene in B(h) individuals.

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Section: Discussionmentioning

confidence: 77%

“…and Pay., presently under study [18]. The H enzyme was not found either with type 1 or with type 2 acceptor substrates (table I).…”

Section: B and H Gene Products In The Serum And The Rbc From Bh Samplesmentioning

confidence: 88%