Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons

Inoue, Yuriko; Honkura, Naoki; Kato, Akihiko; Ogawa, Suchie; Udo, Hiroshi; Inokuchi, Kaoru; Sugiyama, Hiroyuki

doi:10.1016/j.neulet.2003.09.082

Cited by 27 publications

(25 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intriguingly, the PMCA was detected by confocal immunofluorescence microscopy as part of this network [40]. The rapidly inducible expression of short "dominant-negative" forms of Homer (such as Ania-3 and Homer 1a) modulates synaptic signaling by altering metabotropic glutamate receptor trafficking and dissociating functional protein-protein interactions within the signaling complex [11,17,18,20,27]. Similarly, the different Homer splice variants may influence the amplitude and duration of local Ca 2+ signals by reversibly recruiting the PMCAs into these signaling domains.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Homer-1 protein Ania-3 interacts with the plasma membrane calcium pump

Sgambato-Faure

Xiong

Berke

et al. 2006

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The Homer family of scaffold proteins couples NMDA receptors to metabotropic glutamate receptors, and links extracellular signals to calcium release from intracellular stores. Ania-3 is a member of the Homer family and is rapidly inducible in brain in response to diverse stimuli. Here we report the identification of the plasma membrane Ca 2+ ATPase (PMCA) as a novel Ania-3/ Homer-associated protein. Ania-3/Homer interacts with the b-splice forms of all PMCAs (PMCA1b, 2b, 3b, and 4b) via their PDZ domain-binding COOH-terminal tail. Ectopically expressed Ania-3 colocalized with the PMCA at the plasma membrane of polarized MDCK epithelial cells, and endogenous Ania-3/Homer and PMCA2 are co-expressed in the soma and dendrites of primary rat hippocampal neurons. The interaction between Ania-3/Homer and PMCAs may represent a novel mechanism by which local calcium signaling and hence synaptic function can be modulated in neurons.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Homer 1/Ania-3 is highly expressed in the brain where it is enriched in the dendrites and spines of hippocampal neurons [13,27]. PMCA2b is also abundant in the brain and present in the soma and dendrites of hippocampal pyramidal neurons [23,28,29].…”

Section: Ania-3 and Pmca2b Colocalize In Transfected Mdck Cells And Hmentioning

confidence: 99%

The Homer-1 protein Ania-3 interacts with the plasma membrane calcium pump

Sgambato-Faure

Xiong

Berke

et al. 2006

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…In cultured hippocampal neurons, overexpression of Homer1a, or its induction by BDNF, prompts a reduction in long Homer1c clusters without decreasing the amount of protein [75]. Clusters of F-actin and PSD-95 are similarly reduced, through disruption of their association with the scaffolding protein Shank [9, 75, 76], and dendritic spine size is decreased [76]. Hence, by prompting the movement of PSD proteins away from the synapse, Homer1a appears to redistribute the synaptic architecture constructed by long Homer1 proteins in response to neuronal activity.…”

Section: Regulation Of Mglur-nmda Complexesmentioning

confidence: 99%

Regulation and Function of Activity-Dependent Homer in Synaptic Plasticity

et al. 2019

View full text Add to dashboard Cite

Alterations in synaptic signaling and plasticity occur during the refinement of neural circuits over the course of development and the adult processes of learning and memory. Synaptic plasticity requires the rearrangement of protein complexes in the postsynaptic density (PSD), trafficking of receptors and ion channels and the synthesis of new proteins. Activity-induced short Homer proteins, Homer1a and Ania-3, are recruited to active excitatory synapses, where they act as dominant negative regulators of constitutively expressed, longer Homer isoforms. The expression of Homer1a and Ania-3 initiates critical processes of PSD remodeling, the modulation of glutamate receptor-mediated functions, and the regulation of calcium signaling. Together, available data support the view that Homer1a and Ania-3 are responsible for the selective, transient destabilization of postsynaptic signaling complexes to facilitate plasticity of the excitatory synapse. The interruption of activity-dependent Homer proteins disrupts disease-relevant processes and leads to memory impairments, reflecting their likely contribution to neurological disorders.

show abstract

“…By analysis of GFP fluorescence intensity, it was estimated that the typical single postsynaptic site of cultured hippocampal cells contains 343 ± 57 Homer family proteins [34]. Homer1a, induced by brain-derived neurotrophic factor or a proteasome inhibitor, also causes the redistribution of Homer1c [35]. In cultured cerebellar granule cells, endogenous Homer2 and exogenously expressed GFP-fused Homer2 showed a reversible declustering induced by an NMDA receptor-mediated Ca 2+ influx followed by activation of a downstream pathway including mitogen-activated protein (MAP) kinases, extracellular signal-regulated kinases (ERKs) and protein tyrosine kinases [28].…”

Section: Localization and Functionmentioning

confidence: 99%

Untitled

2007

View full text Add to dashboard Cite

SummaryThe Homer family of adaptor proteins consists of three members in mammals, and homologs are also known in other animals but not elsewhere. They are predominantly localized at the postsynaptic density in mammalian neurons and act as adaptor proteins for many postsynaptic density proteins. As a result of alternative splicing each member has several variants, which are classified primarily into the long and short forms. The long Homer forms are constitutively expressed and consist of two major domains: the amino-terminal target-binding domain, which includes an Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) homology 1 (EVH1) domain, and the carboxy-terminal self-assembly domain containing a coiled-coil structure and leucine zipper motif. Multimers of long Homer proteins, coupled through their carboxy-terminal domains, are thought to form protein clusters with other postsynaptic density proteins, which are bound through the amino-terminal domains. Such Homer-mediated clustering probably regulates or facilitates signal transduction or cross-talk between target proteins. The short Homer forms lack the carboxyterminal domain; they are expressed in an activity-dependent manner as immediate-early gene products, possibly disrupting Homer clusters by competitive binding to target proteins. Homer proteins are also involved in diverse non-neural physiological functions.

show abstract

Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons

Cited by 27 publications

References 13 publications

The Homer-1 protein Ania-3 interacts with the plasma membrane calcium pump

The Homer-1 protein Ania-3 interacts with the plasma membrane calcium pump

Regulation and Function of Activity-Dependent Homer in Synaptic Plasticity

Untitled

Contact Info

Product

Resources

About