B erberis is the only genus of the family Berberidaceae, in the southerly hemisphere and comprises 600 species (Bai et al., 2011; Ali et al., 2020). About 24 species are widely distributed in Pakistan's mountainous parts, including Baluchistan, Gilgit, Chitral, Hazara, Murree, Swat, Dir, and Kashmir (Ali et al., 2020). The species is about 1-5 m tall, having deciduous and evergreen shrubs with yellow wood, thorny shoots, and simple leaves. Many species are distinguished for their attractive pink, violet, blue, or dark red color (Arayne et al., 2007). Various species of Berberis are generally dispersed in the temperate and semi-temperate region and are inherent of the whole range of the Himalayas Mountains and North America, Europe, Asia, and the Mediterranean regions (Knapp and Melly, 1986;Chevallier, 2011). The Spe-cies of the Berberis genus are famous for their traditional medicinal use. The evergreen shrub species with the intense pale-yellow color of shoots and roots are used for various ailments, such as rheumatism, stomach disorders diabetes, ear and eye infections, malarial fever, fever, skin disease, and jaundice in remote and rural areas (