Activity Guided Fractionation of Arum italicum Miller Tubers and the LC/MS-MS Profiles

Ağalar, Hale Gamze; Çiftçi, Gülşen Akalın; Göğer, Fatih; Kırımer, N.

doi:10.25135/rnp.06.17.05.089

Cited by 35 publications

(28 citation statements)

References 26 publications

(19 reference statements)

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“…The results obtained in both RP-HPLC-UV and LC-MS /MS are similar to the results of study by Ağalar et al (2017). Ağalar et al (2017) stated that ferulic acid and caffeic acid were present in the leaves of plants belonging to the Araceae family, also p-coumaric acid in the seeds of some species of the same family. Hydroxycinnamic acids containing ferulic acid, caffeic acid, and p-coumaric acid prevent metastasis or invasion of cancer cells (Ağalar et al, 2017).…”

Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/mssupporting

confidence: 86%

“…While ferulic acid, p-coumaric acid, epicatechin, vanillin, caffeic acid, phydroxy benzoic acid and salicylic acid are identified as Phenolic components defined and measured in both RP-HPLC-UV and LC-MS/MS are compatible with each other except for minor differences. Ağalar et al (2017) reported that the whole tuber part of Arum italicum has hydroxycinnamic acid derivatives such as ferulic, caffeic and p-coumaric acid in analysis with LC-MS/MS. The results obtained in both RP-HPLC-UV and LC-MS /MS are similar to the results of study by Ağalar et al (2017).…”

Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/msmentioning

confidence: 99%

“…Ağalar et al (2017) reported that the whole tuber part of Arum italicum has hydroxycinnamic acid derivatives such as ferulic, caffeic and p-coumaric acid in analysis with LC-MS/MS. The results obtained in both RP-HPLC-UV and LC-MS /MS are similar to the results of study by Ağalar et al (2017). Ağalar et al (2017) stated that ferulic acid and caffeic acid were present in the leaves of plants belonging to the Araceae family, also p-coumaric acid in the seeds of some species of the same family.…”

Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/msmentioning

confidence: 99%

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LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

Akar

Demir

Alkan

et al. 2021

Journal of Anatolian Environmental and Animal Sciences

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In the study, the phenolic components and antioxidant activities of edible and nonedible parts of tuber of Arum italicum plant were carried out to determine in methanolic and water extracts. In addition, the antioxidant potential of these parts of the plant was associated with their phenolic composition. Total phenolic content, FRAP, CUPRAC and DPPH tests as radical scavenging activity were performed to determine the antioxidant activity of the plant. The phenolic profiles in the two parts of the tuber were determined in the prepared methanolic extracts by RP-HPLC-UV and LC-MS/MS. The highest values of the total phenolic content, FRAP, CUPRAC and % DPPH were measured in methanolic extracts of nonedible parts of the tuber as 164 µg GAE/mL, 878 TEAC (µM), 0.064 TEAC (µM) and 19.41, respectively. Ferulic acid was determined as the main phenolic compound in methanolic extracts of both the tuber parts by LC/MS-MS. However, luteolin and rutin phenolics were measured as the major compounds in the edible and nonedible parts of the plant tuber with analysis based on RP-HPLC-UV, respectively.

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Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/mssupporting

confidence: 86%

Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/msmentioning

confidence: 99%

Section: Phenolic Component Analysis By Hplc-uv and Lc-ms/msmentioning

confidence: 99%

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LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

Akar

Demir

Alkan

et al. 2021

Journal of Anatolian Environmental and Animal Sciences

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“…Depending upon the fact that no pertinent ion derived due to complete loss of hexosyl unit (−162 amu) was detected, suggested that sugar was C-linked. Thus (4d) was tentatively named as apigenin-C-hexoside-C-hexoside 56,59 . (Fig.…”

Section: Uhplc-q-tof-ms/ms Analysis Uhplc-qtof-ms/ms Was Used For Mementioning

confidence: 99%

UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma

Farooq

Mumtaz

Mukhtar

et al. 2020

Sci Rep

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Butea monosperma is one of the extensively used plants in traditional system of medicines for many therapeutic purposes. in this study, the antioxidant activity, α-glucosidase and α-amylase inhibition properties of freeze drying assisted ultrasonicated leaf extracts (hydro-ethanolic) of B. monosperma have been investigated. The findings revealed that 60% ethanolic fraction exhibited high phenolic contents, total flavonoid contents, highest antioxidant activity, and promising α-glucosidase and αamylase inhibitions. the UHpLc-Qtof-MS/MS analysis indicated the presence of notable metabolites of significant medicinal potential including apigenin, apigenin C-hexoside C-pentoside, apigenin Chexoside C-hexoside, apigenin-6,8-di-C-pentoside and genistin etc., in B. monosperma leave extract. Docking studies were carried out to determine the possible role of each phytochemical present in leaf extract. Binding affinity data and interaction pattern of all the possible phytochemicals in leaf extract of B. monosperma revealed that they can inhibit α-amylase and α-glucosidase synergistically to prevent hyperglycemia. Diabetes mellitus [DM] is most rapidly growing metabolic disorder in the world. It is primarily characterized by hyperglycemia which is associated with disturbed metabolism of carbohydrates, proteins and fats. Such metabolic dysfunctions at physiological level are known to cause detrimental health disorders which lead towards sickness and eventually death 1. According to WHO (World Health Organization), it is estimated that this chronic disease has affected nearly 150 million people throughout the world. This number will increase to three hundred million people or more up to 2025 2. The DM type II (DMT-II) is the most abundant form of diabetes and generally involves the phenomenon of insulin insensitivity or low insulin production. The main reasons behind the spread of this global health problem are mainly modern life style, obesity and consumption of high caloric diet. The growing rate of DM in Asian and African countries is two to three times more than the present rate in other countries 3. The role of reactive oxygen species (ROS) is very crucial in DMT-II pathogenesis. The ROS are produced because of electron transfer to oxygen from mitochondrial metabolic activity. The ROS are captured by antioxidants to maintain the redox homeostasis. However, over production or long-time exposure to ROS may create imbalance which further leads to state of oxidative stress. The oxidative stress exerts harmful impacts on bio-molecules to create metabolic dysfunction. The ROS under umbrella of oxidative stress disturbs the structure based activity of antioxidant enzymes to reduce the antioxidant potential of body 4. The ROS are also involved in impaired insulin secretion from pancreas probably due to dysfunction in β-cells 5. The elevated blood glucose level alters the normal functions of proteins through the process of glycation. The role of glycated end products is obvious in health deterioration and their long term existence...

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“…[51,52] Compound (7) at R t , 1.3 min yielded a deprotonated ion [M-H] − at 387 and further MS-fragment ions at 341, 323 and 233 were also identified in spectrum. A characteristic fragment at 179 revealed that the compound contains caffeic acid, [53] while the fragments at 161 and 131 indicated the cleavage of H 2 O and CH 2 O from the fragment at 179. However, compound (7) was identified tentatively as caffeic acid derivative (Figure 2 and Table 4).…”

Section: Uhplc-qtof-ms/ms Based Metabolite Profile Of C Reflexamentioning

confidence: 99%

UHPLC-QTOF-MS/MS metabolites profiling and antioxidant/antidiabetic attributes of Cuscuta reflexa grown on Casearia tomentosa: exploring phytochemicals role via molecular docking

Nadeem

Mumtaz

Danish

et al. 2020

International Journal of Food Properties

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Present study reports metabolites profiling, antioxidant, and antidiabetic effects of Cuscuta reflexa grown on Casearia tomentosa. The UHPLC-QTOF-MS/MS method was used to characterize phytochemicals in most potent extract. Thirteen bioactive compounds/metabolites including (2S)-3-(β-D-galactopyranosyloxy)-2-(hexanoyloxy)propyl nonanoate, 4-O-β-D-glucosyl-4-coumaric acid, isorhamnetin-3-O-glucoside, 7-(α-D-glucopyranosyloxy)-2,3,4,5,6-pentahydroxyheptanoic acid, 13Shydroxyoctadecadienoic acid, astragaloside derivative, salicylic acid, oleanolic acid, citric acid, pinellic acid, quinic acid, caffeic acid derivative and caffeic acid-3-glucoside were detected in 80% hydroethanolic (HE) extract. Among different extracts (aqueous, 20-80% HE and pure ethanolic), 80% HE extract showed maximum TPC (186.19 ± 2.15 mg GAE/g DE) and TFC (106.50 ± 1.68 mg RE/g DE). A strong correlation was observed between TPC, TFC, DPPH radical scavenging (IC 50 = 60.64 ± 1.74 µg/mL) and TAP activity (235.96 ± 1.33 mg AAE/g DE) of 80% HE extract among others. The same extract also depicted highest α-amylase and α-glucosidase enzyme inhibition with IC 50 of 71.84 ± 1.06 and 57.25 ± 1.40 µg/mL, respectively. Molecular docking was performed to explore possible role of identified phytochemicals. Binding affinity data as well as interaction patterns have revealed possible interaction of the identified bioactives against α-glucosidase and α-amylase. Moreover, intra-molecular charge transfer, energies of the FMOs, HOMO-LUMO-E gaps , MEP and IP were also explained. The present findings suggested that, C. reflexa is a promising natural antioxidant and antidiabetic agent, thus supporting the use of this plant for designing related functional foods and nutra-pharmaceuticals.

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Activity Guided Fractionation of Arum italicum Miller Tubers and the LC/MS-MS Profiles

Cited by 35 publications

References 26 publications

LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma

UHPLC-QTOF-MS/MS metabolites profiling and antioxidant/antidiabetic attributes of Cuscuta reflexa grown on Casearia tomentosa: exploring phytochemicals role via molecular docking

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