Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking

Papadopulos, Andreas; Gómez, Guillermo A.; Martin, Sally; Jackson, Jade; Gormal, Rachel S.; Keating, Damien J.; Yap, Alpha S.; Meunier, Frédéric A.

doi:10.1038/ncomms7297

Cited by 61 publications

(87 citation statements)

References 54 publications

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“…Nanoscissor-induced sudden release of cortical actin tension results in quick recoil of the plasma membrane proximal to the incision point indicating that the actomyosin cortex controls plasma membrane tension (Papadopulos et al, 2015). This effect could not be seen upon pre-treatment with myosin II inhibitor blebbistatin strongly suggesting that the cortical tension mainly depend on actomyosin II activity.…”

Section: Accepted Manuscriptmentioning

confidence: 74%

“…Over time this picture become more complex and dynamic ( Fig. 1), with evidence that the cortical actin network is involved in (i) tethering secretory vesicles (Aschenbrenner et al, 2003;Au et al, 2007;Chibalina et al, 2007;Desnos et al, 2007;Huet et al, 2012;Tomatis et al, 2013), (ii) providing a platform for directed movement towards the plasma membrane (Papadopulos et al, 2015) and (iii) facilitating the generation of new release sites (de Paiva et al, 1999;Zakharenko et al, 1999).…”

Section: +mentioning

confidence: 99%

“…Inhibition of myosin II using blebbistatin also reduced the number of fusion events following stimulation in bovine chromaffin cells (Jackson et al, 2015;Papadopulos et al, 2015) In addition to myosin II, dynamin has emerged as a main regulator of fusion pore size and duration. Furthermore actin and dynamin interplay in opening and closing of the fusion pore also regulates the secretory vesicle fusion process (full fusion to kiss and run exocytosis).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Not only has the cortical actin network been found to influence plasma membrane properties but also to interact with exo-and endocytic vesicles (Gormal et al, 2015;Tomatis et al, 2013). The interplay between actin and membranes is integrally regulated by the phosphoinositide composition of the plasma membrane (Yin and Janmey, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Membrane shaping by actin and myosin during regulated exocytosis

Papadopulos

2017

Molecular and Cellular Neuroscience

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The cortical actin network in neurosecretory cells is a dense mesh of actin filaments underlying the plasma membrane. Interaction of actomyosin with vesicular membranes or the plasma membrane is vital for tethering, retention, transport as well as fusion and fission of exo-and endocytic membrane structures. During regulated exocytosis the cortical actin network undergoes dramatic changes in morphology to accommodate vesicle docking, fusion and replenishment. Most of these processes involve plasma membrane Phosphoinositides (PIP) and investigating the interactions between the actin cortex and secretory structures has become a hotbed for research in recent years. Actin remodelling leads to filopodia outgrowth and the creation of new fusion sites in neurosecretory cells and actin, myosin and dynamin actively shape and maintain the fusion pore of secretory vesicles. Changes in viscoelastic properties of the actin cortex can facilitate vesicular transport and lead to docking and priming of vesicle at the plasma membrane. Small GTPase actin mediators control the state of the cortical actin network and influence vesicular access to their docking and fusion sites. These changes potentially affect membrane properties such as tension and fluidity as well as the mobility of embedded proteins and could influence the processes leading to both exo-and endocytosis. Here we discuss the multitudes of actin and membrane interactions that control successive steps underpinning regulated exocytosis.

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Section: Accepted Manuscriptmentioning

confidence: 74%

Section: +mentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Membrane shaping by actin and myosin during regulated exocytosis

Papadopulos

2017

Molecular and Cellular Neuroscience

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“…Inhibition of the RhoA-ROCK pathway might favor the SNARE complex formation by preventing tomosyn/syntaxin-1 interaction. Alternatively, it might regulate the level of myosin light chain (MLC) phosphorylation and therefore modulate the myosin-induced forces required for exocytosis [32] . These two hypotheses are not mutually exclusive and required further investigations.…”

Section: Oligophrenin-1 a New Actor In Calcium-regulated Exocytosismentioning

confidence: 99%

Oligophrenin-1: the link between calcium-regulated exocytosis and compensatory endocytosis in neuroendocrine cells

et al. 2016

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In neuroendocrine cells, hormones and neuropeptides are released from large-dense core vesicles (secretory granules) by calcium-regulated exocytosis. Following exocytosis, compensatory uptake of membrane is required to maintain membrane homeostasis and allow recycling of secretory vesicle membranes. How these cells initiate and regulate this compensatory endocytosis remains poorly understood. Our recent data suggests that oligophrenin-1 (OPHN1) is a link coupling calcium-regulated exocytosis to compensatory endocytosis of secretory granules in the adrenal chromaffin cells (Houy et al., 2015, J Neurosci. 2015, 35:11045-55). Here, we highlight the major evidence and discuss how OPHN1 could couple these two processes.

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The axonal radial contractility: Structural basis underlying a new form of neural plasticity

et al. 2021

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Axons are the longest cellular structure reaching over a meter in the case of human motor axons. They have a relatively small diameter and contain several cytoskeletal elements that mediate both material and information exchange within neurons.Recently, a novel type of axonal plasticity, termed axonal radial contractility, has been unveiled. It is represented by dynamic and transient diameter changes of the axon shaft to accommodate the passages of large organelles. Mechanisms underpinning this plasticity are not fully understood. Here, we first summarised recent evidence of the functional relevance for axon radial contractility, then discussed the underlying structural basis, reviewing nanoscopic evidence of the subtle changes. Two models are proposed to explain how actomyosin rings are organised. Possible roles of non-muscle myosin II (NM-II) in axon degeneration are discussed. Finally, we discuss the concept of periodic functional nanodomains, which could sense extracellular cues and coordinate the axonal responses. Also see the video abstract here: https://youtu.be/ojCnrJ8RCRc

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Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking

Cited by 61 publications

References 54 publications

Membrane shaping by actin and myosin during regulated exocytosis

Membrane shaping by actin and myosin during regulated exocytosis

Oligophrenin-1: the link between calcium-regulated exocytosis and compensatory endocytosis in neuroendocrine cells

The axonal radial contractility: Structural basis underlying a new form of neural plasticity

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