Activity Determination of Amino Acid Decarboxylases in Enterobacteriaceae

Møller, Vagn

doi:10.1111/j.1699-0463.1954.tb00808.x

Cited by 34 publications

(16 citation statements)

References 2 publications

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“…Møller (1954) studied that the distribution of the decarboxylases of lysine and ornithine differs from the different species of enterobacteria. Diamines, putrescine and cadaverine, are usually common amines often related to the activity of enterobacteria.…”

Section: Resultsmentioning

confidence: 99%

Biogenic amine production by bacteria isolated from ice-preserved sardine and mackerel

et al. 2012

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a b s t r a c tThe occurrence of in vitro production of biogenic amines in bacteria isolated from ice-preserved sardine and mackerel was studied. Biogenic amine production was investigated by means of amino acid decarboxylation by growth on decarboxylase differential medium, biogenic amine detection by thinlayer chromatography (TLC) and decarboxylase gene detection by PCR. Decarboxylase medium overestimate the number of biogenic amine-producer strains, as the production of amine was confirmed by TLC in only five out the 17 presumptive strains. On the producer strains, PCR was used to confirm the presence of the genes encoding the amino acid decarboxylase responsible for the synthesis of these amines. Moreover, biogenic amine-producer bacteria were molecularly identified by sequencing their 16S rRNA. Form sardine, enterobacteria producing simultaneously several biogenic amines were isolated. A Kluyvera intermedia strain producing histamine, putrescine and cadaverine, and an Enterobacter asburiae strain producing only the diamines cadaverine and putrescine were identified. From mackerel, lactic acid bacteria from the Enterococcus durans species producing tyramine were isolated. This study constitutes the first description of the presence on these putatively harmful species on ice-preserved sardine and mackerel.

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Section: Resultsmentioning

confidence: 99%

Biogenic amine production by bacteria isolated from ice-preserved sardine and mackerel

et al. 2012

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“…Phenylalanine deaminase activity was determined by flooding phenylalanine agar (HiMedia) after 24 h incubation with 1 % (w/v) ferric chloride solution. Decarboxylase activity of amino acids L-tyrosine and L-valine) were detected using decarboxylase broth base (Møller; HiMedia) as described by Møller (1954). Biochemical characteristics were determined using the Hi25 Enterobacteriaceae identification kit (HiMedia) and parts A, B and C of the HiCarbohydrate kit (HiMedia), according to the manufacturer's instructions.…”

mentioning

confidence: 99%

Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

Gupta

Dharne

Rangrez

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1T and FFA3T, and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1T) and 99.35 % (FFA3T) to Ignatzschineria larvae L1/68T. The level of gene sequence similarity between strains FFA1T and FFA3T was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1T and FFA3T shared 24 % DNA–DNA relatedness. DNA–DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1T and 44 % for strain FFA3T) and I. larvae L1/68T genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1T and FFA3T, respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), CyC19 : 0ω8c and C14 : 0. The results of DNA–DNA hybridization between the two new strains and I. larvae L1/68T, in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1T and FFA3T represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1T = DSM 22309T = KCTC 22643T = NCIM 5325T) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3T = DSM 22310T = KCTC 22644T = NCIM 5326T) are proposed.

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“…1 In 1954, Möller first described this genus and suggested the name Hafnia alvei. 2 The genus name Hafnia is the historical name (Havn) for the city of Copenhagen, Denmark and the species name alvei (derived from Latin) means "of a beehive". 1,3 Literature search of Pubmed, Embase, Medline and Google scholar were done using the words: neonate, sepsis and Hafnia alvei.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3] In 1988, H. alvei infection was first reported in neonates in a 20-day old preterm (30 weeks) baby with sepsis and NEC. 4 Compared to adult population, few isolated cases of H. alvei infection were reported in neonates.…”

Section: Introductionmentioning

confidence: 99%

Hafnia alvei causing late onset sepsis in neonates: report of two cases and review of literature

Basani¹,

Aepala²

2018

Int J Contemp Pediatr

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Hafnia alvei, a Gram negative motile bacillus that belongs to Enterobacteriaceae family is rarely associated with infection in pediatric patients and is exceptionally rare in the neonatal period. H. alvei is ubiquitous in the environment, causing infections in debilitated and immuno-compromised patients with few cases being reported in neonates. We report two cases of late onset sepsis in term neonates caused by H. alvei that were successfully treated in our unit. To the best of our knowledge, infection due to H. alvei has not been reported in neonates from India. Hafnia alvei causes infection rarely in neonates. Because it can cause nosocomial outbreaks, awareness regarding this uncommon pathogen and initiation of appropriate antibiotic therapy improves the outcome and prevents mortality.

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Activity Determination of Amino Acid Decarboxylases in Enterobacteriaceae

Cited by 34 publications

References 2 publications

Biogenic amine production by bacteria isolated from ice-preserved sardine and mackerel

Biogenic amine production by bacteria isolated from ice-preserved sardine and mackerel

Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

Hafnia alvei causing late onset sepsis in neonates: report of two cases and review of literature

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