Activity‐dependent regulation of the potassium channel subunits Kv1.1 and Kv3.1

Lu, Yong; Monsivais, Pablo; Tempel, Bruce L.; Rubel, Edwin W.

doi:10.1002/cne.11037

Cited by 86 publications

(82 citation statements)

References 74 publications

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“…This is in contrast to the observation by Lu et al (2004); Kv1.1 labeling in NL was predominant in the neuronal soma rather than in the neuropil, and the difference may be attributable to the different polyclonal antibody raised against Kv1.1. The neurons in NM showed a slight gradient of Kv1.2 immunoreactivity (Fig.…”

Section: Expression Of Kv1 Channels In Nlcontrasting

confidence: 99%

Tonotopic Specialization of Auditory Coincidence Detection in Nucleus Laminaris of the Chick

Kuba

Yamada²,

Fukui³

et al. 2005

J. Neurosci.

113

145

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The interaural time difference (ITD) is a cue for localizing a sound source along the horizontal plane and is first determined in the nucleus laminaris (NL) in birds. Neurons in NL are tonotopically organized, such that ITDs are processed separately at each characteristic frequency (CF). Here, we investigated the excitability and coincidence detection of neurons along the tonotopic axis in NL, using a chick brainstem slice preparation. Systematic changes with CF were observed in morphological and electrophysiological properties of NL neurons. These properties included the length of dendrites, the input capacitance, the conductance of hyperpolarization-activated current, and the EPSC time course. In contrast to these gradients, the conductance of low-threshold K ϩ current and the expression of Kv1.2 channel protein were maximal in the central (middle-CF) region of NL. As a result, the middle-CF neuron had the smallest input resistance and membrane time constant, and consequently the fastest EPSP, and exhibited the most accurate coincidence detection. The specialization of middle-CF neurons as coincidence detectors may account for the high resolution of sound-source localization in the middle-frequency range observed in avians.

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Section: Expression Of Kv1 Channels In Nlcontrasting

confidence: 99%

Tonotopic Specialization of Auditory Coincidence Detection in Nucleus Laminaris of the Chick

Kuba

Yamada²,

Fukui³

et al. 2005

J. Neurosci.

113

145

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show abstract

“…Antibodies for C terminal of mouse Kv1.1 protein were obtained from Alomone Labs (APC-009). The staining pattern by this antibody was similar to that in the study by Lu et al (2004). Four posthatched chickens (P3, P8, P10, and P11) were perfused through the heart with 10% formalin-PBS, and brains were dissected out, postfixed for 2 hr in 10% formalin-PBS, and kept overnight in 30% sucrose-PBS.…”

Section: Methodssupporting

confidence: 64%

“…In rat, immunolabeling to Kv1.1 showed a gradient across the tonotopic axis in the lateral superior olive (LSO), which underlies the firing properties of principal neuron (Barnes-Davies et al, 2004). However, tonotopic gradient of Kv1.1 protein was not clearly demonstrated in the NM of the chicken (Lu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis

Fukui¹,

Ohmori²

2004

J. Neurosci.

144

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Nucleus magnocellularis (NM) is a division of the avian cochlear nucleus that extracts the timing of auditory signals. We compared the membrane excitability and synaptic transmission along the tonotopic axis of NM. Neurons expressed a Kv1.1 potassium channel mRNA and protein predominantly in the high characteristic frequency (CF) region of NM. In contrast, the expression of Kv1.2 mRNA did not change tonotopically. Neurons also showed tonotopic gradients in resting potential, spike threshold, amplitude, and membrane rectification. All neurons were sensitive to 100 nM dendrotoxin, but the effects were most significant in the high CF neurons. The EPSC recorded by minimal stimulation of auditory nerve fibers (ANFs) was 13 times larger in high CF neurons than in low CF neurons. Moreover, EPSCs were generated in an all-or-none manner in the high CF neurons when stimulus intensity was increased, whereas EPSCs were graded in the low CF neurons, indicating multiple axonal inputs. ANF synaptic terminals were visualized by DiI. ANF formed enfolding end-bulbs of Held around the cell body in the high and middle CF region but not in the low CF region. These observations indicate coordinated gradients of neuronal properties both presynaptically and postsynaptically along the tonotopic axis. Such specializations may be suitable for extracting and preserving the timing information of auditory signals over a wide range of acoustic frequencies.

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“…While the acoustic environment has been shown to influence auditory brain stem responses through modulation of voltage-gated potassium channels, this is achieved largely through phosphorylation rather than chronic alterations in gene expression (Chambard and Ashmore, 2005;Kaczmarek et al, 2005;Macica et al, 2003;Song et al, 2005). Studies in the avian cochlear nucleus (nucleus magnocellularis) have shown large deafness-related changes in Kv1.1 and Kv3.1 expression following cochlear ablation (Lu et al, 2004;von Hehn et al, 2004). However, these changes are transient (Lu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Studies in the avian cochlear nucleus (nucleus magnocellularis) have shown large deafness-related changes in Kv1.1 and Kv3.1 expression following cochlear ablation (Lu et al, 2004;von Hehn et al, 2004). However, these changes are transient (Lu et al, 2004). Similarly, the expression of Kv1.1 and Kv1.2 in the rat cochlear nucleus is unchanged 10 days after cochlear ablation (Caminos et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Deafness associated changes in expression of two-pore domain potassium channels in the rat cochlear nucleus

et al. 2006

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Two-pore domain potassium channels ( ) play an important role in setting resting membrane potential by regulating background leakage of potassium ions, which in turn controls neuronal excitability. To determine whether these channels contribute to activity-dependent plasticity following deafness, we used quantitative real-time PCR to examine the expression of 10 subunits in the rat cochlear nucleus at 3 days, 3 weeks and 3 months after bilateral cochlear ablation. There was a large sustained decrease in the expression of TASK-5, a subunit that is predominantly expressed in auditory brain stem neurons, and in the TASK-1 subunit which is highly expressed in several types of cochlear nucleus neurons. TWIK-1 and THIK-2 also showed significant decreases in expression that were maintained across all time points. TWIK-2, TREK-1 and TREK-2 showed no significant change in expression at 3 days but showed large decreases at 3 weeks and 3 months following deafness. TRAAK and TASK-3 subunits showed significant decreases at 3 days and 3 weeks following deafness, but these differences were no longer significant at 3 months. Dramatic changes in expression of subunits suggest these channels may play a role in deafness-associated changes in the excitability of cochlear nucleus neurons.

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Activity‐dependent regulation of the potassium channel subunits Kv1.1 and Kv3.1

Cited by 86 publications

References 74 publications

Tonotopic Specialization of Auditory Coincidence Detection in Nucleus Laminaris of the Chick

Tonotopic Specialization of Auditory Coincidence Detection in Nucleus Laminaris of the Chick

Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis

Deafness associated changes in expression of two-pore domain potassium channels in the rat cochlear nucleus

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