Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site

Lee, M. Hope; Clingenpeel, Scott; Leiser, Owen P.; Wymore, Ryan A.; Sorenson, Kent S.; Watwood, Mary E.

doi:10.1016/j.envpol.2007.07.034

Cited by 19 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The model assumed first‐order degradation and was calibrated to concentrations observed at the two monitoring wells (~21 and 160 µg/L, respectively), resulting in estimated biodegradation half‐lives of 8.25 and 1.5 years within the aerobic plume, respectively. These values are consistent with other field‐scale estimates for aerobic TCE degradation half‐life of 5 to 30 years (e.g., Sorenson et al ; Wymore et al ; Lee et al ). The higher estimated rate to the south/southwest was attributed to different hydrogeological characteristics between the east and south‐southwest areas of the aquifer.…”

Section: Resultssupporting

confidence: 91%

Performance Assessment of Pump‐and‐Treat Systems

Truex¹,

Johnson²,

Macbeth³

et al. 2017

Groundwater Monitoring Rem

View full text Add to dashboard Cite

Pump‐and‐treat (P&T) is a widely applied remedy for groundwater remediation at many types of sites for multiple types of contaminants. Decisions regarding major changes in the remediation approach are an important element of environmental remediation management for a site using P&T. While existing guidance documents provide information on design, operation, and optimization for P&T systems, these documents do not provide specific technical guidance to support remedy decisions regarding when to transition to a new remedy or to initiate closure of the P&T remedy. A structured approach for P&T performance assessment was developed and is described herein, using analysis of three example P&T systems. These examples highlight key aspects of the performance assessment decision logic and represent assessment outcomes associated with optimizing the P&T system, transitioning from P&T to natural attenuation, and supplementing P&T with another technology to hasten transition to natural attenuation.

show abstract

Section: Resultssupporting

confidence: 91%

Performance Assessment of Pump‐and‐Treat Systems

Truex¹,

Johnson²,

Macbeth³

et al. 2017

Groundwater Monitoring Rem

View full text Add to dashboard Cite

show abstract

“…The presence of this activity in groundwater from the TAN site strongly supports the hypothesis of Sorenson et al (2000) that TCE is undergoing natural transformation in situ via aerobic cometabolic oxidation. Additional studies with probes targeting aromatic oxygenases confirm the presence of cometabolic activity in this aquifer (Lee et al, 2007); these studies also provided direct evidence of TCE degradation concomitant with probe activity by indigenous microbial populations from TAN.…”

Section: Discussionmentioning

confidence: 69%

Field Evidence for Intrinsic Aerobic Chlorinated Ethene Cometabolism by Methanotrophs Expressing Soluble Methane Monooxygenase

Wymore¹,

Lee²,

Keener³

et al. 2007

Bioremediation Journal

Self Cite

View full text Add to dashboard Cite

Idaho National Laboratory's Test Area North is the site of a trichloroethene (TCE) plume resulting from waste injections. Previous investigations revealed that TCE was being attenuated relative to two codisposed internal tracers, tritium and tetrachloroethene, with a half-life of 9 to 21 years. Biological attenuation mechanisms were investigated using a novel suite of assays, including enzyme activity probes designed for the soluble methane monooxygenase (sMMO) enzyme. Samples were analyzed for chlorinated solvents, tritium, redox parameters, primary substrates, degradation products, bacterial community methanotrophic potential, and bacterial DNA. The enzyme probe assays, methanotrophic enrichments and isolations, and DNA analysis documented the presence and activity of indigenous methanotrophs expressing the sMMO enzyme. Three-dimensional groundwater data showed plume-wide aerobic conditions, with low levels of methane and detections of carbon monoxide, a by-product of TCE cometabolism. The TCE half-life attributed to aerobic cometabolism is 13 years relative to tritium, based on the tracer-corrected method. Similarly, a half-life of 8 years was estimated for cisdichloroethene (DCE). Although these rates are slower than most anaerobic degradation processes, they can be significant for large plumes. This investigation is believed to be the first documentation of intrinsic aerobic TCE and DCE cometabolism in an aquifer by indigenous methanotrophs.

show abstract

“…Lee and colleagues (Lee et al. ; Wymore et al. ) evaluated biological attenuation mechanisms using a novel suite of assays, including DNA, enrichment cultures, and enzyme activity probes, to reveal that TCE was being co‐metabolized by indigenous microorganisms and significantly contributing to the attenuation of the contaminant.…”

Section: Remedy Implementation and Managementmentioning

confidence: 99%

Idaho National Laboratory Test Area North: Application of Endpoints to Guide Adaptive Remediation at a Complex Site

Lee

Truex²,

Freshley³

et al. 2016

Remediation

Self Cite

View full text Add to dashboard Cite

Complex sites in the subsurface are defined as those with difficult access, deep, and/or thick zones of contamination, large areal extent, heterogeneities that limit the effectiveness of remediation, or where long‐term remedies are needed to address contamination (e.g., because of long‐term sources or large extent). The Test Area North at the Idaho National Laboratory, developed for nuclear fuel operations and heavy metal manufacturing, is a complex site that demonstrates the endpoints strategy for adaptive remediation. Liquid wastes and sludge from experimental facilities were disposed in an injection well, which contaminated the subsurface aquifer located deep within fractured basalt. The mixed wastes included organic, inorganic, and low‐level radioactive constituents, with the focus of this study on the remediation of trichloroethylene within a systems‐based, regulatory framework. The framework facilitates site, regulator, and stakeholder interactions during the remedial planning and implementation process by using a conceptual model description as a technical foundation for decisions identifying endpoints. Endpoints are defined as interim remediation targets or decision points on the path to an ultimate end, and maintaining protectiveness during the remediation process. Results demonstrate that the three‐component remedy used at Test Area North as an example of the structured framework is largely functioning as intended and is projected to meet remedial action objectives by 2095. The remedy approach is being adjusted as new data become available. The framework provides a structured process for evaluating and adjusting the remediation approach, allowing site owners, regulators, and stakeholders to manage contamination at complex sites where adaptive remedies are needed. ©2016 Wiley Periodicals, Inc.

show abstract

Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site

Cited by 19 publications

References 38 publications

Performance Assessment of Pump‐and‐Treat Systems

Performance Assessment of Pump‐and‐Treat Systems

Field Evidence for Intrinsic Aerobic Chlorinated Ethene Cometabolism by Methanotrophs Expressing Soluble Methane Monooxygenase

Idaho National Laboratory Test Area North: Application of Endpoints to Guide Adaptive Remediation at a Complex Site

Contact Info

Product

Resources

About