Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain

Lardelli, Michael; Key, Brian; Koblar, Simon; Lewis, Martin

doi:10.3389/fnana.2014.00148

Cited by 16 publications

(15 citation statements)

References 76 publications

(109 reference statements)

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“…The olfactory system exhibited robust expression, shortly after birth, with other brain areas following thereafter. A similar expression pattern has been described in other vertebrate species including the developing zebrafish brain (Klarić et al, 2014 ) GABAergic and glutamatergic.…”

Section: Discussionsupporting

confidence: 78%

Expression of Npas4 mRNA in Telencephalic Areas of Adult and Postnatal Mouse Brain

2015

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The transcription factor neuronal PAS domain-containing protein 4 (Npas4) is an inducible immediate early gene which regulates the formation of inhibitory synapses, and could have a significant regulatory role during cortical circuit formation. However, little is known about basal Npas4 mRNA expression during postnatal development. Here, postnatal and adult mouse brain sections were processed for isotopic in situ hybridization using an Npas4 specific cRNA antisense probe. In adults, Npas4 mRNA was found in the telencephalon with very restricted or no expression in diencephalon or mesencephalon. In most telencephalic areas, including the anterior olfactory nucleus (AON), piriform cortex, neocortex, hippocampus, dorsal caudate putamen (CPu), septum and basolateral amygdala nucleus (BLA), basal Npas4 expression was detected in scattered cells which exhibited strong hybridization signal. In embryonic and neonatal brain sections, Npas4 mRNA expression signals were very low. Starting at postnatal day 5 (P5), transcripts for Npas4 were detected in the AON, CPu and piriform cortex. At P8, additional Npas4 hybridization was found in CA1 and CA3 pyramidal layer, and in primary motor cortex. By P13, robust mRNA expression was located in layers IV and VI of all sensory cortices, frontal cortex and cingulate cortex. After onset of expression, postnatal spatial mRNA distribution was similar to that in adults, with the exception of the CPu, where Npas4 transcripts became gradually restricted to the most dorsal part. In conclusion, the spatial distribution of Npas4 mRNA is mostly restricted to telencephalic areas, and the temporal expression increases with developmental age during postnatal development, which seem to correlate with the onset of activity-driven excitatory transmission.

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Section: Discussionsupporting

confidence: 78%

Expression of Npas4 mRNA in Telencephalic Areas of Adult and Postnatal Mouse Brain

2015

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“…S4B). Murine NPAS4 is known to regulate synaptic function (Spiegel et al, 2014), and npas4a expression has previously been described to be restricted to the zebrafish brain, similar to mammalian Npas4 (Klaric et al, 2014). We have previously reported that npas4l is expressed specifically in the LPM during early somitogenesis using in situ hybridization (Reischauer et al, 2016).…”

Section: Transcriptomic and Epigenomic Analyses Of Npas4l Mutantsmentioning

confidence: 87%

Genome-wide strategies reveal target genes of Npas4l associated with vascular development in zebrafish

et al. 2019

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The development of a vascular network is essential to nourish tissues and sustain organ function throughout life. Endothelial cells (ECs) are the building blocks of blood vessels, yet our understanding of EC specification remains incomplete. Zebrafish cloche/npas4l mutants have been used broadly as an avascular model, but little is known about the molecular mechanisms of action of the Npas4l transcription factor. Here, to identify its direct and indirect target genes, we have combined complementary genome-wide approaches, including transcriptome analyses and chromatin immunoprecipitation. The cross-analysis of these datasets indicates that Npas4l functions as a master regulator by directly inducing a group of transcription factor genes that are crucial for hematoendothelial specification, such as etv2, tal1 and lmo2. We also identified new targets of Npas4l and investigated the function of a subset of them using the CRISPR/Cas9 technology. Phenotypic characterization of tspan18b mutants reveals a novel player in developmental angiogenesis, confirming the reliability of the datasets generated. Collectively, these data represent a useful resource for future studies aimed to better understand EC fate determination and vascular development.

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“…In human, the NPAS4 transcript was mainly expressed in brain tissue, though, unlike in rodents, no expression was observed in the testis [ 5 ]. This brain-specific expression pattern seems to be conserved even in lower vertebrates as demonstrated by our recent study which showed that, in the adult zebrafish, the expression of the zebrafish Npas4 homolog npas4a was also restricted to the brain [ 42 ].…”

Section: Expression Of Npas4mentioning

confidence: 99%

The Role of the Neuroprotective Factor Npas4 in Cerebral Ischemia

Choy

Klarić

Koblar

et al. 2015

IJMS

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Stroke is one of the leading causes of death and adult disability in the world. Although many molecules have been documented to have a neuroprotective effect, the majority of these molecules failed to improve the neurological outcomes for patients with brain ischemia. It has been proposed that neuroprotection alone may, in fact, not be adequate for improving the prognosis of ischemic stroke. Neuroprotectants that can regulate other processes which occur in the brain during ischemia could potentially be targets for the development of effective therapeutic interventions in stroke. Neuronal Per-Arnt-Sim domain protein 4 (Npas4) is an activity-dependent transcription factor whose expression is induced in various brain insults, including cerebral ischemia. It has been shown that Npas4 plays an important role in protecting neurons against many types of neurodegenerative insult. Recently, it was demonstrated that Npas4 indeed has a neuroprotective role in ischemic stroke and that Npas4 might be involved in modulating the cell death pathway and inflammatory response. In this review, we summarize the current knowledge of the roles that Npas4 may play in neuroinflammation and ischemia. Understanding how ischemic lesion size in stroke may be reduced through modulation of Npas4-dependent apoptotic and inflammatory pathways could lead to the development of new stroke therapies.

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Activity-dependent expression of neuronal PAS domain-containing protein 4 (npas4a) in the developing zebrafish brain

Cited by 16 publications

References 76 publications

Expression of Npas4 mRNA in Telencephalic Areas of Adult and Postnatal Mouse Brain

Expression of Npas4 mRNA in Telencephalic Areas of Adult and Postnatal Mouse Brain

Genome-wide strategies reveal target genes of Npas4l associated with vascular development in zebrafish

The Role of the Neuroprotective Factor Npas4 in Cerebral Ischemia

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