Activity Coefficients and Dissociation of Lead Chloride in Aqueous Solutions.

Garrels, Robert M.; Gucker, Frank T.

doi:10.1021/cr60137a008

Cited by 13 publications

(3 citation statements)

References 17 publications

(30 reference statements)

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“…2-D protein profiles contain large amounts of information on the physical characteristics ( P I and molecular weight) and amounts of the proteins present in the sample. A number of computer systems [61,62] have been developed for data processing that could be applied to epidemiological studies. Computerised densitometry has been used for the statistical analysis of 1-D protein profiles in bacterial taxonomy [63] and for developing computerised databases for the identification of bacteria and viruses on the basis of the 1-D protein profiles [64-661.…”

Section: Data Analysis and The Significance Of Protein Variations Detmentioning

confidence: 99%

“…For many epidemiological studies only the PI and molecular weight values of the proteins in the virus or bacterial isolates need to be recorded, i.e. the Xand Ycoordinates of the protein spot on the protein profile.These may in turn be cross-referenced to a "Master" 2-D protein profile in a computer database [62]. During the collection of these data, effort should be made to determine the significance of the demonstrated protein variation.…”

Section: Data Analysis and The Significance Of Protein Variations Detmentioning

confidence: 99%

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The application of two‐dimensional polyacrylamide gel electrophoresis to medical microbiology: Molecular epidemiology of viruses and bacteria

Cash

1991

Electrophoresis

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A variety of molecular methods can be used to identify protein and nucleic acid markers with which to investigate the epidemiology of viruses and bacteria. This paper reviews the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) for studying microbial molecular epidemiology. A small format 2-D PAGE system is described for locating protein markers in group B coxsackie viruses (CVB) and Haemophilus influenzae isolates. Representative isolates of CVB serotypes 2, 4, and 5 were compared by analysing the intracellular proteins present in CVB-infected HEp-2 cells by 2-D PAGE protein gels. Although some of the virus-induced proteins had similar electrophoretic mobilities, the three serotypes could be distinguished from each other on the basis of a major virus-induced protein of molecular weight between 39,000 and 43,000. Protein differences were demonstrated among six serotype 2 CVB (CVB-2) isolates. Four clinical CVB-2 isolates collected over a period of four months had indistinguishable two-dimensional protein profiles. Comparison of the two-dimensional protein profiles of cloned virus stocks prepared from a single clinical CVB isolate demonstrated that it was a heterogeneous virus population. The proteins of nontypable and type-b H. influenzae isolates were compared. Up to 160 proteins, detected by staining with Coomassie Brilliant Blue R, were resolved by 2-D PAGE. Although protein differences between individual bacterial isolates were detected, comparable two-dimensional protein profiles were found for the two groups of H. influenzae isolates. There was no similarity in the two-dimensional protein profiles of H. influenzae and Aeromonas. Potential protein markers were identified that may be useful in long-term studies of H. influenzae epidemiology.

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Section: Data Analysis and The Significance Of Protein Variations Detmentioning

confidence: 99%

Section: Data Analysis and The Significance Of Protein Variations Detmentioning

confidence: 99%

The application of two‐dimensional polyacrylamide gel electrophoresis to medical microbiology: Molecular epidemiology of viruses and bacteria

Cash

1991

Electrophoresis

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“…Soc., 70, 3745 (1948). , (129) Kolthoff, I. M., and Bovey, F. A., Ibid., p. 791. (130) Matheson, M. S., Auer, E. E., Bevilaequa, E, B., and Hart, E. J., J.…”

Section: Booksmentioning

confidence: 99%