Activity changes in gill ion transporter enzymes in response to salinity and temperature in fathead minnows (Pimephales promelas)

Monroe, Ian; Wentworth, Simon; Thede, Katrina; Aravindabose, Varsha; Garvin, Jeffrey L.; Packer, Randall K.

doi:10.1016/j.cbpa.2018.10.018

Cited by 8 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been demonstrated that the gill tissue improves active ion absorption capacity and resists ion loss by increasing the expression levels of NHE3 , CLC-2 , and AE2, when fishes migrate from high-salt environment to freshwater [ 15 , 47 – 49 ]. Numerous previous studies have shown that NKA-α expression decreases when water temperature increases [ 22 , 50 , 51 ], this was substantiated by the change results of heat stress at 28℃ and 24℃ when compared to 20℃ in our study (Fig. 8 B).…”

Section: Resultssupporting

confidence: 90%

Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii)

Yang

Lin

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

Background Maintaining osmotic equilibrium plays an important role in the survival of cold-water fishes. Heat stress has been proven to reduce the activity of Na+/K+-ATPase in the gill tissue, leading to destruction of the osmotic equilibrium. However, the mechanism of megatemperature affecting gill osmoregulation has not been fully elucidated. Results In this study, Siberian sturgeon (Acipenser baerii) was used to analyze histopathological change, plasma ion level, and transcriptome of gill tissue subjected to 20℃, 24℃and 28℃. The results showed that ROS level and damage were increased in gill tissue with the increasing of heat stress temperature. Plasma Cl− level at 28℃ was distinctly lower than that at 20℃ and 24℃, while no significant difference was found in Na+ and K+ ion levels among different groups. Transcriptome analysis displayed that osmoregulation-, DNA-repair- and apoptosis-related terms or pathways were enriched in GO and KEGG analysis. Moreover, 194 osmoregulation-related genes were identified. Amongst, the expression of genes limiting ion outflow, occluding (OCLN), and ion absorption, solute carrier family 4, member 2 (AE2) solute carrier family 9, member 3 (NHE3) chloride channel 2 (CLC-2) were increased, while Na+/K+-ATPase alpha (NKA-a) expression was decreased after heat stress. Conclusions This study reveals for the first time that the effect of heat stress on damage and osmotic regulation in gill tissue of cold-water fishes. Heat stress increases the permeability of fish’s gill tissue, and induces the gill tissue to keep ion balance through active ion absorption and passive ion outflow. Our study will contribute to research of global-warming-caused effects on cold-water fishes.

show abstract

Section: Resultssupporting

confidence: 90%

Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii)

Yang

Lin

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that the gill improves active ion absorption capacity and resists ion loss by increasing the expression levels of NHE3, CLC-2, and AE2, when sh migrate from a high-salt environment to freshwater [8, [52][53][54]. Numerous previous studies have shown that NKA-α expression decreases when water temperature increases [17,55,56], this was substantiated by the change results of 28℃ and 24℃ when compared to 20℃ in our study (Fig. 5B).…”

Section: Discussionsupporting

confidence: 86%

Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish

Yang

Lin

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Maintaining osmotic balance plays an important role in the normal operation and survival of cold-water fish. High water temperature has been proven to reduce the activity of NA+-K+-ATPase in the gill. However, the mechanism of megatemperature affect gill osmoregulation has not been fully elucidated. Results In this study, A.baerii, as a kind of iconic cold-water fish, was used to analyze histological alterations, plasma ion content, and transcriptional responses of gill of A.baerii, subjected to 20℃, 24℃and 28℃. We found that ROS levels in gill tissue increased and the gill damage deteriorated along with the temperature rising. Moreover, plasma Cl− levels at 28℃ were much lower than those in the other two groups, and there were no significant differences in Na+ and K+ ions among all trials. At the same time, the transcriptome data indicated that there were significant changes in injury-related pathways and genes at 24℃and 28℃. Meanwhile, GO enrichment analysis showed a large number of genes related to ion transport function changed with temperature increasing during cell processes. We discovered that as temperature increased, the expression of genes that regulate ion outflow (OCLN) and active ion absorption (AE2, NHE3, CLC-2) increased, while NKA-a expression decreased. Furthermore, the changes in osmotic regulation genes and the degree of tissue damage at 28℃ were significantly higher than those in the other two groups, indicating that the degree of heat stress-induced injury affects the osmotic regulation function of gill tissue. Conclusions this study reveals for the first time that the tissue damage caused by high temperature affects the function and the response mechanism of the osmotic regulation in gill of cold-water fish. It provides reference data for the study on the physiological function of cold-water fish under global warming.

show abstract

“…In addition, some plant residues with high water content need to be dried by various methods (such as freeze-drying, solar drying) before extracting NEP in order to reduce the water activity, lipid oxidation rate, and enzymatic activity. It can also help prevent the lipid-rich substrate from triggering oxidation reactions under the action of lipoxygenase and phenol oxidase, which can happen in the subsequent high-temperature treatment to generate free radicals that may degrade polyphenols easily. , It should be noted that the choice of drying method can also impact the polyphenol content of the matrix, for example, studies have shown that the freeze-drying process retains more polyphenols than air-drying . After pretreatment, it is necessary to determine the appropriate extraction procedures according to the chemical properties of the target extract, such as the particle size of the matrix, the properties of interfering substances, and other characteristics.…”

Section: Extraction Of Polyphenols From Food Processing Wastementioning

confidence: 99%

Valorization of Food Processing Waste to Produce Valuable Polyphenolics

Chang

Shi

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Traditional incineration and landfill of food processing waste (FPW) have polluted the environment and underutilized valuable bioactive compounds, including polyphenols in food waste. As one of the most widely occurring compounds in the FPW, polyphenols possess high utilization value in many fields such as human health, energy, and environmental protection. Extracting polyphenols directly from FPW can maximize the value of polyphenols and avoid waste of resources. However, traditional polyphenol extraction methods mostly use the Soxhlet extraction, infiltration, and impregnation method, consuming a large amount of organic solvent and suffering from long extraction time and low extraction efficiency. Emerging green extraction methods such as supercritical fluid extraction, ultrasonic-assisted extraction, microwave-assisted extraction, and other methods can shorten the extraction time and improve the solvent extraction efficacy, resulting in the green and safe recovery of polyphenols from FPW. In this paper, the traditional treatment methods of FPW waste and the application of polyphenols in FPW are briefly reviewed, and the traditional extraction methods and emerging green extraction methods of polyphenols in FPW are compared to obtain insight into the start-of-the-art extraction approaches.

show abstract

Activity changes in gill ion transporter enzymes in response to salinity and temperature in fathead minnows (Pimephales promelas)

Cited by 8 publications

References 25 publications

Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii)

Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii)

Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish

Valorization of Food Processing Waste to Produce Valuable Polyphenolics

Contact Info

Product

Resources

About