Activity‐Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image‐Guided Surgery

Li, Haidong; Kim, Dayeh; Yao, Qichao; Ge, Haoying; Chung, Jeewon; Fan, Jiangli; Wang, Jingyun; Peng, Xiaojun; Yoon, Juyoung

doi:10.1002/ange.202009796

Cited by 50 publications

(29 citation statements)

References 204 publications

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“…In particular, NIR fluorescent probes have the advantage of minimum interference from background fluorescence, result in the minimum photodamage, and have consequently been extensively used for the real-time imaging of cells, tissues, and live systems. 62 − 65 Thus, the fluorescence spectral characteristics for previous NTR fluorescent probes have been collated to help guide the choice of an appropriate target NIR probe.…”

Section: Results and Discussionmentioning

confidence: 99%

2D Strategy for the Construction of an Enzyme-Activated NIR Fluorophore Suitable for the Visual Sensing and Profiling of Homologous Nitroreductases from Various Bacterial Species

et al. 2021

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Nitroreductases (NTRs) mediate the reduction of nitroaromatic compounds to the corresponding nitrite, hydroxylamine, or amino derivatives. The activity of NTRs in bacteria facilitates the metabolic activation and antibacterial activity of 5-nitroimidazoles. Therefore, NTR activity correlates with the drug susceptibility and resistance of pathogenic bacteria. As such, it is important to develop a rapid and visual assay for the real-time sensing of bacterial NTRs for the evaluation and development of antibiotics. Herein, an activatable near-infrared fluorescent probe ( HC–NO 2 ) derived from a hemicyanine fluorophore was designed and developed based on two evaluation factors, including the calculated partition coefficient (Clog P ) and fluorescence wavelength. Using HC–NO 2 as the special substrate of NTRs, NTR activity can be assayed efficiently, and then, bacteria can be imaged based on the detection of NTRs. More importantly, a sensitive in-gel assay using HC–NO 2 has been developed to selectively identify NTRs and sensitively determine NTR activity. Using the in-gel assay, NTRs from various bacterial species have been profiled visually from the “fluorescence fingerprints”, which facilitates the rapid identification of NTRs from bacterial lysates. Thus, various homologous NTRs were identified from three metronidazole-susceptible bacterial species as well as seven unsusceptible species, which were confirmed by the whole-genome sequence. As such, the evaluation of NTRs from different bacterial species should help improve the rational usage of 5-nitroimidazole drugs as antibiotics.

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Section: Results and Discussionmentioning

confidence: 99%

2D Strategy for the Construction of an Enzyme-Activated NIR Fluorophore Suitable for the Visual Sensing and Profiling of Homologous Nitroreductases from Various Bacterial Species

et al. 2021

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“…Generally, the fluorescence and PA signals of probes are quenched by reason adjustment of spatial distance or structural transformations before reacting with the target. When the recognition site of the probe interacts specifically with bioactive molecules, the optical signal is restored by the formed new structure owing to the enhancement of the intramolecular charge transfer (ICT) mechanism, [27] or the hindrance of photon‐induced electron transfer [28] . Moreover, the obtained compounds commonly possess a remarkable NIR absorption signal and are suitable for PA imaging [25,29] .…”

Section: Design Strategies For Small Molecule Fluorescence and Pa Dua...mentioning

confidence: 99%

“…When the recognition site of the probe interacts specifically with bioactive molecules, the optical signal is restored by the formed new structure owing to the enhancement of the intramolecular charge transfer (ICT) mechanism, [27] or the hindrance of photon-induced electron transfer. [28] Moreover, the obtained compounds commonly possess a remarkable NIR absorption signal and are suitable for PA imaging. [25,29] This is commonly effective in avoiding interference from other non-target analytes, making these probes more suitable for biological detection in complicated environments.…”

Section: Design Strategies For Small Molecule Fluorescence and Pa Dua...mentioning

confidence: 99%

Recent Progress in Small‐Molecule Fluorescence and Photoacoustic Dual‐Modal Probes for the In‐Vivo Detection of Bioactive Molecules

Zhou

Zhang

Wang

et al. 2022

Chemistry An Asian Journal

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Intracellular bioactive molecules are essential for the maintenance of homeostasis in living organisms. Abnormal levels of them are closely related to the occurrence and development of some diseases. Hence, the direct and accurate visualization of these bioactive molecules is of vital importance for exploring their pathological roles. However, the low-content, short-lived, and widely distributed properties of bioactive molecules impede the comprehensive analysis of them dramatically. Fluorescence and photoacoustic dual-mode imaging technology provides a new solution to the above issue. Specifically, the combination of fluorescence and photoacoustic, which possesses the advantages of high resolution and in-depth tissue analysis, enables a more in-depth and systematic exploration of the pathogenic mechanisms of bioactive molecules. Moreover, due to the structural tailorability of small molecule probes, numerous small molecule dual-mode probes have been developed to meet the demand for real-time tracking and visualization of bioactive molecules in living cells or in vivo. Hence, in this review, we briefly summarize the key advances in small molecule fluorescence and photoacoustic dual-modal probes within recent years (2015)(2016)(2017)(2018)(2019)(2020)(2021). A particular focus is placed on the design strategies and biological applications of probes for the detection of various bioactive molecules in vivo. Furthermore, the challenges and further prospects in this hot field are highlighted.

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“…Over the last few decades, great progress has been made in achieving highly efficient OLED devices, resulting in the commercialization of television, smartphone, and wearable devices 12–16 . Wherein, red light is not only an indispensable part of three primary colors for full‐color displays and white OLED but also widely applied in the field of optical telecommunication, information secured devices, night‐vision displays, and bioimaging 17–24 . Although there are rapid advances in organic light‐emitting materials with various colors through rational molecular design, 25–40 the development of red and near‐infrared (NIR) materials is much slower due to the intrinsic narrow bandgap and aggregation‐caused quenching (ACQ) effect 41–43 …”

Section: Introductionmentioning

confidence: 99%

Aggregation‐induced emission: Red and near‐infrared organic light‐emitting diodes

Xie

et al. 2021

SmartMat

112

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Red and near‐infrared (NIR) organic light‐emitting diodes (OLED) have gained remarkable interest due to their numerous applications. However, the construction of highly emissive emitters is hampered by the energy‐gap law and aggregation‐caused quenching (ACQ) effect. Whereas, aggregation‐induced emission (AIE) materials could avoid the undesirable ACQ effect and emit bright light in aggregated state, which is one class of the most promising materials to fabricate high‐performance OLED with a high external quantum efficiency and low efficiency roll‐off. This review summarizes recent advances in red and NIR OLED with AIE property, including the traditional fluorescence, thermally activated delayed fluorescence, and hybridized local and charge transfer compounds. Meanwhile, the emphasis attention is paid to the molecular design principles, as well as the molecular structure‐photophysical characteristics. We also briefly further outlook the challenges and perspective of red and NIR AIE luminogens.

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Activity‐Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image‐Guided Surgery

Cited by 50 publications

References 204 publications

2D Strategy for the Construction of an Enzyme-Activated NIR Fluorophore Suitable for the Visual Sensing and Profiling of Homologous Nitroreductases from Various Bacterial Species

2D Strategy for the Construction of an Enzyme-Activated NIR Fluorophore Suitable for the Visual Sensing and Profiling of Homologous Nitroreductases from Various Bacterial Species

Recent Progress in Small‐Molecule Fluorescence and Photoacoustic Dual‐Modal Probes for the In‐Vivo Detection of Bioactive Molecules

Aggregation‐induced emission: Red and near‐infrared organic light‐emitting diodes

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