SUMMARY. The effects of inhibition of endogenous prostaglandin synthesis on the release of norepinephrine from sympathetic nerves and on postjunctional adrenergic responsiveness were studied in isolated canine left circumflex coronary arteries. In rings, suspended for isometric tension recording and contracted with prostaglandin F 2o , transmural electrical stimulation caused frequency-dependent relaxations, which were blocked by propranolol and augmented by indomethacin. In superfused strips, previously incubated with [ HJnorepinephrine, electrical stimulation (2 Hz) increased the overflow of tritiated neurotransmitter; indomethacin did not influence basal or evoked [ 3 H]norepinephrine overflow. Exogenous norepinephrine caused relaxations in rings contracted with prostaglandin F 2o , but increases in tension in potassium-depolarized tissues which could be abolished by phentolamine; isoproterenol induced relaxations in both cases. Indomethacin significantly augmented the relaxation in response to exogenous norepinephrine (during contractions with prostaglandin F 2(t ) and reversed norepinephrine-induced contractions (during potassium-depolarization) into relaxation. Other cyclooxygenase inhibitors had comparable effects. In the presence of propranolol, indomethacin did not diminish contractions evoked by norepinephrine in depolarized rings. Relaxations induced by sodium nitroprusside or acetylcholine during contractions caused by prostaglandin F 2<> or potassium chloride were not affected by indomethacin. The augmentation of ^-adrenergic responsiveness by indomethacin was abolished by exogenous prostacyclin. The prostacyclin synthetase inhibitor tranylcypromine and exogenous prostaglandin E2 depressed /3-adrenergic responsiveness. Indomethacin did not affect the facilitatory action of phosphodiesterase inhibition on /3-adrenergic relaxation. The data suggest that endogenous prostaglandins (most probably prostacyclin and prostaglandin E 2 ) exert a "braking" effect on /3-adrenergic responsiveness in coronary arterial smooth muscle. (Circ Res 56: 117-125, 1985) THE large coronary arteries of the canine heart contain adrenergic nerve terminals which release norepinephrine upon stimulation; under control conditions, the /3-adrenergic effect of the released transmitter predominates, causing relaxation (Borda et al., 1977;Rorie and Shepherd, 1980;Cohen et al., 1983). The relaxations of the canine coronary artery induced by electrical stimulation of the adrenergic nerve endings or by the indirectly acting sympathomimetic amine, tyramine, are potentiated by the inhibitor of cyclooxygenase, indomethacin (Cohen et al., 1983). These observations suggest that endogenously formed prostaglandins impair adrenergic neuroeffector interaction in this preparation by either decreasing the release of norepinephrine from the adrenergic nerve endings, by accelerating the disposition of the transmitter, by increasing a-adrenergic responsiveness of the vascular smooth muscle or by reducing /3-adrenergic relaxation. The present stud...