Activity and Point Mutation G699V in PcoORP1 Confer Resistance to Oxathiapiprolin inPhytophthora colocasiaeField Isolates

Abstract

The oxysterol-binding protein inhibitor oxathiapiprolin is a new fungicide for controlling oomycetes diseases. Besides, laboratory mutagenesis oxathiapiprolin-resistance among phytopathogenic oomycetes in the field remains unknown. Here, the sensitivity of 97 P. colocasiae isolates to oxathiapiprolin was examined that were collected between 2011 and 2016. We obtained a baseline sensitivity with a mean EC50 value of 5.2639 × 10–4 μg mL–1. We showed that 6/32 isolates collected in Fujian Province from 2019 to 2020 were resistant to oxathiapiprolin without a significant fitness penalty on sporulation, vegetative growth, and virulence of the field isolates. The oxathiapiprolin resistance field isolates contained the point mutation glycine to valine at 699 in PcoORP1. The point mutation G699V was verified to confer resistance of P. colocasiae to oxathiapiprolin using the CRISPR/Cas9 system. The mutation G699V decreased the binding affinity between oxathiapiprolin and PcoORP1. These results will improve our understanding of the mechanism of P. colocasiae resistance to oxathiapiprolin under field conditions.

Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenicPhytophthoraspecies

“…Pang & R. Liu [12], P. infestans [15], and P. colocasiae Racib. [13]. However, it is possible that the RPL41 promoter is more active in P. cactorum compared to the other four species we tested.…”

“…The continued development of molecular DNA techniques and sequencing technologies in the last 20-30 years has shifted Phytophthora research increasingly towards genetics and genomics studies with the intent of elucidating the genetic mechanisms that drive phytopathogenicity and other related traits [2][3][4][5][6][7]. The development of CRISPR-Cas9 gene editing [8] and related technologies has provided new tools for the genetic exploration of phytopathogenicity, and CRISPR-Cas9 protocols have now been developed for several agricultural Phytophthora pathogens [9][10][11][12][13]. CRISPR-Cas gene editing technologies have proven to be invaluable in elucidating the genetic mechanisms driving Phytophthora-host interactions and phytopathogenicity [9,10,14,15], and exploring fungicide resistance [11,13,16].…”

“…The development of CRISPR-Cas9 gene editing [8] and related technologies has provided new tools for the genetic exploration of phytopathogenicity, and CRISPR-Cas9 protocols have now been developed for several agricultural Phytophthora pathogens [9][10][11][12][13]. CRISPR-Cas gene editing technologies have proven to be invaluable in elucidating the genetic mechanisms driving Phytophthora-host interactions and phytopathogenicity [9,10,14,15], and exploring fungicide resistance [11,13,16]. However, CRISPR-Cas9 gene editing has yet to be established in any forest pathogenic Phytophthoras, likely due to the lack of stable and efficient DNA transformation protocols in these species.…”

A New Insight into 6-Pentyl-2H-pyran-2-one against Peronophythora litchii via TOR Pathway

“…healthtech.dtu.dk./service.hph?SignalP-4.1, accessed on 22 December 2021), and then homology modeling was processed and analyzed for applicability by SWISS-MODEL (https://swissmodel.expasy.org, accessed on 22 December 2021) and SAVES v.6.0 (https: //saves.mbi.ucla.edu, accessed on 22 December 2021). Furthermore, autodock software (version 1.5.6) and python software (version 2.5) was used for docking analyses using the default parameters to calculate the binding affinities between ligand and receptors, and 50 interaction models were accepted to establish with default docking parameter [34]. Interaction models were visualized by Discovery Studio software (version 4.5), and five replicates were used.…”