Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

Henderson, Clare A.; McLiesh, Heather; Then, Whui Lyn; Garnier, Gil

doi:10.3389/fchem.2018.00193

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…False negative reporting was also studied, and it was seen that antibody in liquid form had swelling of cellulose fibers due to moisture content that decreased erythrocyte adhesion to paper; thus the occurrence of a false positive was low, while air-dried antibodies and lyophilized antibodies showed false positive results after 9 months. Increased BSA concentration reduced protein (antibody) adsorption resulting in better flow, and thus a clearer negative was obtained for BSA diluted antibodies . Hertaeg et al explored a paper-based device for reverse typing using paper towel and hand sheet as the substrate for the detection of IgM blood typing antibodies and SARS CoV-2 antibodies.…”

Section: Different Paper Substratesmentioning

confidence: 99%

“…Increased BSA concentration reduced protein (antibody) adsorption resulting in better flow, and thus a clearer negative was obtained for BSA diluted antibodies. 70 Hertaeg et al explored a paper-based device for reverse typing using paper towel and hand sheet as the substrate for the detection of IgM blood typing antibodies and SARS CoV-2 antibodies. Reagent blood cells were mixed with the patient's plasma, which contains the antibodies to be identified and deposited on paper.…”

Section: Different Paper Substratesmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidics-Based Blood Typing Devices: An In-Depth Overview

Jayachandran,

Parween,

Asthana

et al. 2023

ACS Appl. Bio Mater.

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Identification of correct blood types holds paramount importance in understanding the pathophysiological parameters of patients, therapeutic interventions, and blood transfusion. Considering the wide applications of blood typing, the requirement of centralized laboratory facilities is not well suited on many occasions. In this context, there has been a significant development of such blood typing devices on different microfluidic platforms. The advantages of these microfluidic devices offer easy, rapid test protocols, which could potentially be adapted in resource-limited settings and thereby can truly lead to the decentralization of testing facilities. The advantages of pump-free liquid transport (i.e., low power consumption) and biodegradability of paper substrates (e.g., reduction in medical wastes) make it a more preferred platform in comparison to other microfluidic devices. However, these devices are often coupled with some inherent challenges, which limit their potential to be used on a mass commercial scale. In this context, our Review offers a succinct summary of the recent development, especially to understand the importance of underlying facets for long-term sustainability. Our Review also delineates the role of integration with digital technologies to minimize errors in interpreting the readouts.

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Section: Different Paper Substratesmentioning

confidence: 99%

Section: Different Paper Substratesmentioning

confidence: 99%

Microfluidics-Based Blood Typing Devices: An In-Depth Overview

Jayachandran,

Parween,

Asthana

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…Through a glutaraldehyde cross-linking agent, the antibodies showed enhanced binding activity to the target when compared to the periodate oxidation method [ 97 ]. Other methods for antibody immobilization on a paper with shelf-life up to 12 months have been described [ 98 , 99 ]. Polyclonal antibodies have multiple binding sites, each specific to a particular antigen.…”

Section: Recognition Elementmentioning

confidence: 99%

Role of Paper-Based Sensors in Fight against Cancer for the Developing World

et al. 2022

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Cancer is one of the major killers across the globe. According to the WHO, more than 10 million people succumbed to cancer in the year 2020 alone. The early detection of cancer is key to reducing the mortality rate. In low- and medium-income countries, the screening facilities are limited due to a scarcity of resources and equipment. Paper-based microfluidics provide a platform for a low-cost, biodegradable micro-total analysis system (µTAS) that can be used for the detection of critical biomarkers for cancer screening. This work aims to review and provide a perspective on various available paper-based methods for cancer screening. The work includes an overview of paper-based sensors, the analytes that can be detected and the detection, and readout methods used.

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“…Though paper-based diagnostics are reliable and costeffective, the shelf-life of such products is of concern to many users. A study was done to examine the effects of aging on antibody activity on paper and in solutions under different aging and drying conditions such as airdried, lyophilized, and kept as a liquid [30] . It showed that paper kept wet with undiluted antibodies has the longest shelf-life and the clearest reading.…”

Section: Microfluidic Paper-based Analytical Device (µPad)mentioning

confidence: 99%

Microchip technology applications for blood group analysis

Gao¹,

Misevic²

2020

Blood and Genomics

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Blood group analysis techniques are some of the most in demand immunological applications in clinical transfusion praxis and organ transplantation. In order to aid the advance towards higher throughput and increased sensitivity, analytical solutions dealing with a minimal amount of blood samples and the miniaturization of diagnostic equipment using microchip technologies have been evolving into an optimal solution. Here we review fabrication technologies for various types of microstructure on microchips, related operating procedures, and characterization approaches. Our focus is on examples of microchip technology and instrumentation used for blood group analysis ranging from classical serological methods of glycoprotein detection and solid phase assays, to nucleic acid amplification techniques. Molecular typing using microchip-based techniques is emerging as a supplement to standard serological methods. Microchip technology will play its key role to support blood group analysis at the molecular scale by using microliters of blood samples for extremely sensitive, quantitative, and high throughput analyses.

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Activity and Longevity of Antibody in Paper-Based Blood Typing Diagnostics

Cited by 12 publications

References 21 publications

Microfluidics-Based Blood Typing Devices: An In-Depth Overview

Microfluidics-Based Blood Typing Devices: An In-Depth Overview

Role of Paper-Based Sensors in Fight against Cancer for the Developing World

Microchip technology applications for blood group analysis

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