Activity and characterization of secondary metabolites produced by a new microorganism for control of plant diseases

Efecto de biocontrol e inhibición germinativa de Bacillus spp. sobre zoosporas de Phytophthora capsici

“…Different microorganisms have been reported to suppress P. capsici growth, including Streptomyces spp. (Ko et al, 2010;Nguyen et al, 2012), Paenibacillus spp. (Naing et al, 2014), Trichoderma sp.…”

“…To induce P. capsici to produce sporangia, 5-mm agar disks with mycelium of the pathogen that had been growing for 5 days were taken and placed on Petri dishes to which 10 mL of distilled water were added, and incubated at 25-27°C for 48 h. To release the zoospores from the sporangia, the Petri dishes were incubated at 4°C for 30 min (Ko et al, 2010) and a concentration of 20 zoospores/µL was obtained. Each slide concavity was filled with 20 µL of the zoospore suspension to which 20 µL of bacterial cell suspension of B17 and B32 isolates at a concentration of 1 x 10 8 colony forming units (CFU) 1:1 v/v were added, covered with a slide to prevent evaporation and incubated at 25-27°C for 24 h (Ko et al, 2010). The zoospores that were not inoculated with the bacterial cell suspension were used as a control.…”

“…Para inducir la formación de esporangios de P. capsici, se tomaron discos de 5 mm de agar con micelio del patógeno con 5 días de crecimiento, éstos se colocaron en cajas Petri con 10 mL de agua destilada e incubada a 25-27°C por 48 h. Para la liberación de zoosporas del esporangio, las cajas Petri se incubaron a 4°C por 30 min (Ko et al, 2010), logrando una concentración de 20 zoosporas/µL. Se colocaron 20 µL de suspensión de zoosporas en cada concavidad del portaobjeto, se añadió 20 µL de suspensión celular bacteriana de los aislados B17 y B32 a una concentración de 1 x 10 8 UFC 1:1 v/v, se cubrió con un cubreobjetos para prevenir la evaporación, y se incubó por 24 h a 25-27°C (Ko et al, 2010). Las zoosporas que no fueron inoculadas con la suspensión celular bacteriana se utilizaron como testigo.…”

“…The cultures were filtered using 0.22 µm Millipore ® filters to remove bacterial cells (Chen et al, 2016b). To inhibit germination, 20 µL of the zoospore suspension (20 zoospores/µL), mixed with 20 µL of bacterial filtrate (1:1 v/v), were placed in the slide cavity, covered with a slide to prevent evaporation and then incubated at 25-27°C for 24 h (Ko et al, 2010). Zoospores without bacterial filtrate were used as a control.…”

“…Los cultivos se filtraron utilizando filtros Millipore ® de 0.22 µm para eliminar las células bacterianas (Chen et al, 2016b). Para la inhibición de la germinación, se colocaron 20 µL de suspensión de zoosporas (20 zoosporas/µL) en la cavidad del portaobjeto, mezclado con 20 µL del filtrado bacteriano (1:1 v/v), se cubrieron con cubreobjetos para prevenir la evaporación y se incubaron por 24 h a 25-27 °C (Ko et al, 2010). Las zoosporas sin filtrado bacteriano se utilizaron como testigo.…”