2002
DOI: 10.1038/sj.ijo.0801981
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Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice

Abstract: The effect of the ob=ob mutation on microsomal overt and latent acyl CoA:diacylglycerol acyl transferase activities (DGATs I and II) in liver microsomes of mice was investigated. Overt and latent hepatic DGAT activities for 16-week-old lean Ob=? mice were 0.42 AE 0.14 and 1.57 AE 0.04 mmol=mg=min, respectively. For ob=ob mice DGAT I and II activities were 1.17 AE 0.28 and 3.09 AE 0.78 mmol=mg=min, respectively. The hepatic triacylglycerol (TAG) concentration of 16-week-old mice was increased three-fold in ob=o… Show more

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Cited by 18 publications
(8 citation statements)
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“…This model agrees with previous biochemical studies in which the active site of DGAT (and that of the family member acyl-CoA:monoacylglycerol acyltransferase) was oriented toward the cytosol (24). A latent DGAT activity (present only after treating membranes with disrupting agents) has been detected in rat liver microsomes (25)(26)(27)(28), suggesting that there are active sites on both sides of the ER membrane. Our topology data suggest that DGAT2 is not responsible for this latent activity.…”
Section: Discussionsupporting
confidence: 81%
“…This model agrees with previous biochemical studies in which the active site of DGAT (and that of the family member acyl-CoA:monoacylglycerol acyltransferase) was oriented toward the cytosol (24). A latent DGAT activity (present only after treating membranes with disrupting agents) has been detected in rat liver microsomes (25)(26)(27)(28), suggesting that there are active sites on both sides of the ER membrane. Our topology data suggest that DGAT2 is not responsible for this latent activity.…”
Section: Discussionsupporting
confidence: 81%
“…Increased expression of the gene for DGAT has been shown to promote VLDL secretion in the McA RH7777 cell line, but it also results in cellular accumulation of TG (20). In leptin-deficient (ob/ob) mice, a mouse model of diet-induced obesity and insulin resistance, both hepatic dgat1 and dgat2 activities are increased more than twofold compared with wild-type controls in conjunction with an upregulation of hepatic srebp1 gene (35) despite an absence of the sterol response element in their promoters. These mice also develop increased hepatic lipogenesis, accumulation of hepatic TG, and hepatic VLDL overproduction.…”
Section: Discussionmentioning
confidence: 99%
“…Likewise, the reduction in hepatic TG content in the face of increased lipogenesis and TG production suggests a role for additional regulatory factors. PUFA circulate mainly in the form of PL and CE, with those in TG and nonesterified fatty acid (NEFA) forming a minor component (35). The nearly complete absence of PUFA-CE in the apoB-containing lipoproteins in both ldlr Ϫ/Ϫ ϫlcat Ϫ/Ϫ and lcat Ϫ/Ϫ mice would likely impact on the hepatic abundance of PUFA as exogenous source.…”
Section: Discussionmentioning
confidence: 99%
“…This difference is also supported by previous reports on rat liver and yeast DGAT enzymes. Two types of DGAT activities were detected from liver microsomes: one is overt and regulates the cytosolic TAG pools, and the other is latent and plays a role in TAG secretion (22,23). In yeast cells, inactivation of the yeast DGAT1 gene resulted in the reduction of DGAT activity on lipid particles, but without significant effect on DGAT activity in the ER (24).…”
Section: Discussionmentioning
confidence: 99%