Activities of N-acetyl-β-d-glucosaminidase and glutathione peroxidase in bovine colostrum and milk

Abstract

Parturition and post partum period are susceptible for antioxidative/oxidative imbalance as well as inflammatory processes related to either uterus or mammary gland. Fifteen Holstein-Friesian cows were used to examine the relationship between the antioxidant enzyme glutathione peroxidase (GSH-Px) and inflammatory enzyme N-acetyl-glucosaminidase (NAGase) in colostrum and milk during 12 days after parturition. Samples from each udder quarter were collected immediately after parturition, after 24 h and 48 h as well as after 6 and 12 days post partum. The activity of GSH-Px was measured spectrophotometrically and NAGase spectrofluorimetrically. NAGase activity generally remained constant during the examined period confirming no inflammation and healthy udder. Moreover, no significant differences in the activity among quarters were detected. The activity of GSH-Px rose significantly (P < 0.05) in the examined period suggesting the dynamic balance of antioxidative defence. No differences among quarters confirmed that in healthy quarters metabolic processes are on a similar level but positive correlations between the antioxidative and inflammatory enzyme in quarters and with regard to time may suggest a possible relationship during inflammation.

Tannery Effluent Treatment by Microfiltration through Ceramic Membrane for Water Reuse: Assessment of Environmental Impacts

“…The various non‐enzymatic and enzymatic antioxidants present in the tissues as a part of anti‐oxidative defense mechanism of organisms serves the role of ROS scavenger. This response of the defense mechanism of organisms against stress results in increased activity of anti‐oxidative enzymes to combat loss of enzymes . From Table it might be observed that the APx activity increased to about 1.84‐fold in Pistia sp.…”