Activities of cytochrome c oxidase and mitochondrial lactate dehydrogenase isozymes and Cox1, Cox2, Cox4, and Cox6 gene subunit expression in cold adaptation of Salmo trutta L.

Meshcheryakova, O. V.; Churova, M. V.; Веселов, А. Е.; Немова, Н. Н.

doi:10.1134/s1068162016010106

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…Our results also predict a relevant increase in the number of days with zero flow for some stations in the detrital area under this scenario (RCP8.5). The predicted streamflow changes are compatible with those obtained in previous studies, although these studies were performed at larger scales (Milly et al, 2005;van Vliet et al, 2013). The apparent differences between the streamflow reductions estimated in this study and those obtained by Milly et al (2005) and van Vliet et al (2013) (who report lower flow reductions than those given in the present study) might be caused by the regional focus of their predictions (the entire Iberian Peninsula), whereas ours are focused on mountain reaches.…”

Section: Streamflowsupporting

confidence: 87%

“…At the regional level, a reduction in water resources is expected in the Mediterranean area (IPCC, 2013). Milly et al (2005) predicted a 10-30 % decrease in runoff in southern Europe in 2050. In another global-scale study, van Vliet et al (2013) predicted a decrease in the mean flows of greater than 25 % in the Iberian Peninsula area by the end of the century (2071-2100), using averages for both the SRES A2 and B1 scenarios (Nakicenovic et al, 2000).…”

Section: Streamflowmentioning

confidence: 99%

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Saez¹

2017

Preprint

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Climate changes affect aquatic ecosystems by altering temperatures and precipitation patterns, and the rear edges of the distributions of cold-water species are especially sensitive to these effects. The main goal of this study was to predict in detail how changes in air temperature and precipitation will affect streamflow, the thermal habitat of a cold-water fish (the brown trout, Salmo trutta), and the synergistic relationships among these variables at the rear edge of the natural distribution of brown trout. Thirty-one sites in 14 mountain rivers and streams were studied in central Spain. Models of streamflow were built for several of these sites using M5 model trees, and a non-linear regression method was used to estimate stream temperatures. Nine global climate models simulations for Representative Concentration Pathways RCP4.5 and RCP8.5 scenarios were downscaled to the local level. Significant reductions in streamflow were predicted to occur in all of the basins (max. −49 %) by the year 2099, and seasonal differences were noted between the basins. The stream temperature models showed relationships between the model parameters, geology and hydrologic responses. Temperature was sensitive to streamflow in one set of streams, and summer reductions in streamflow contributed to additional stream temperature increases (max. 3.6 • C), although the sites that are most dependent on deep aquifers will likely resist warming to a greater degree. The predicted increases in water temperatures were as high as 4.0 • C. Temperature and streamflow changes will cause a shift in the rear edge of the distribution of this species. However, geology will affect the extent of this shift. Approaches like the one used herein have proven to be useful in planning the prevention and mitigation of the negative effects of climate change by differentiating areas based on the risk level and viability of fish populations.Published by Copernicus Publications on behalf of the European Geosciences Union.

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Section: Streamflowsupporting

confidence: 87%

Section: Streamflowmentioning

confidence: 99%

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Saez¹

2017

Preprint

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“…Previously it was thought that the enzyme was present only in the cytoplasm, but now there is robust evidence that the enzyme is also found in the mitochondria (mLDH). Lactate can be transferred to the mitochondrial matrix via a lactate transporter protein (MCT1) and oxidized there to pyruvate by mLDH, which provides pyruvate to oxidative processes and to gluconeogenesis in the matrix (Storey, 2015;Meshcheryakova et al, 2016). LDH is composed of A and B subunits, that forms homo-or heterotetramers in fish and higher animals.…”

Section: Introductionmentioning

confidence: 99%

Isozymes of malate-and lactate dehydrogenase of Astyanax bimaculatus as biomarkers of environmental impact

et al. 2019

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The activity of certain isozymes may serve as biomarkers of specific physiological conditions of living organisms. The present work aimed to evaluate malate dehydrogenase (MDH) and lactate dehydrogenase (LDH) isozymes from the hepatic, branchial and renal tissues of the fish Astyanax bimaculatus as biochemical markers of environmental changes in the Una River Basin (Taubaté, SP, Brazil). For this study, the specimens were collected in water bodies located in three sampling sites of the basin, called P1 (Itaim stream), P2 (a stream along the Remédios municipal road) and P3 (a small lake near the Dr. José Luiz Cembranelli municipal road). Physicochemical analysis of the water from these sites indicated that P1 was the less polluted site, P2 presented high levels of electrolytes in water, and P3 presented the worst water quality among the sampling sites. The isoenzymes were separated by native-PAGE and identified by their activity on the polyacrylamide gel. The results indicated that four isozymes of MDH occur in the liver of these fish, and the activities of MDH-1 and MDH-4 were detected only in fish from P2, suggesting that these isoforms have potential as biomarkers of the presence of high levels of electrolytes in water. In the kidney, MDH-1 was detected in fish from P2 and P3, but it was not detected in those from P1, suggesting that the presence of this isozyme may be a biomarker of low-quality water. The gill MDH and LDH isozyme profiles of all tissues examined showed some similarities between individuals from the three collection sites, indicating that they are not suitable as biomarkers of the environmental conditions of these sites.

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“…in fish energy balance, affecting the rate of food intake, metabolic rate and growth performance (Forseth et al, 2009;Elliott and Elliott, 2010;Elliott and Allonby, 2013). It is also involved in many other physiological functions such as blood and reproductive maturation (Jeffries et al, 2012), reproductive timing (Warren et al, 2012), gametogenesis (Lahnsteiner and Leitner, 2013), cardiac function (Vornanen et al, 2014), gene expression (White et al, 2012;Meshcheryakova et al, 2016), ecological relationships (Hein et al, 2013;Fey and Herren, 2014), and fish behaviour (Colchen et al, 2016). 5…”

mentioning

confidence: 99%

Waning habitats due to climate change: effects of streamflow and temperature changes at the rear edge of the distribution of a cold-water fish

Saez¹,

Muñoz‐Mas²,

Solana³

et al. 2017

Preprint

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Climate change affects aquatic ecosystems altering temperature and precipitation patterns, and the rear edge of the distribution of cold-water species is especially sensitive to them. The main goal was to predict in detail how change in air temperature and precipitation will affect streamflow, the thermal habitat of a cold-water fish (brown trout, Salmo trutta Linnaeus 1758), and their synergistic relationships at the rear edge of its natural distribution. 31 sites in 14 mountain rivers 15 and streams were studied in Central Spain. Models at several sites were built using regression trees for streamflow, and a non-linear regression method for stream temperature. Nine global climate models simulations for the RCP4.5 and RCP8.5 (Representative Concentration Pathways) scenarios were downscaled to a local level. Significant streamflow reductions were predicted in all basins (max. -49 %) by the year 2099, showing seasonal differences between them. The stream temperature models showed relationships between models parameters, geology and hydrologic responses. Temperature was sensitive to 20 the streamflow in one set of streams, and summer reductions contributed to additional stream temperature increases (max.3.6ºC), although the most deep-aquifer dependent sites better resisted warming. The predicted increase in water temperature reached up to 4.0ºC. Temperature and streamflow changes will cause a shift of the rear edge of the species distribution.However, geology conditioned the extent of this shift. Approaches like these should be useful in planning the prevention and mitigation of negative effects of climate change by differentiating areas based on the risk level and viability of fish 25 populations.

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Activities of cytochrome c oxidase and mitochondrial lactate dehydrogenase isozymes and Cox1, Cox2, Cox4, and Cox6 gene subunit expression in cold adaptation of Salmo trutta L.

Cited by 8 publications

References 25 publications

response to comments

response to comments

Isozymes of malate-and lactate dehydrogenase of Astyanax bimaculatus as biomarkers of environmental impact

Waning habitats due to climate change: effects of streamflow and temperature changes at the rear edge of the distribution of a cold-water fish

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