Activities of cyclooxygenases, and levels of prostaglandins E2 And F2α, in fetopathy associated with experimental diabetic gestation

Al-Matubsi, Hisham Y.; Salim, M.; El-Sharaky, A.S.; Kamel, Maher A.; Oriquat, Ghaleb A.; Helmy, Mai M.; El-Bassiouni, E. A.; Shourbaji, M.

doi:10.1016/j.diabet.2009.06.009

Cited by 14 publications

(17 citation statements)

References 42 publications

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“…It has been suggested that COX-2, but not COX-1, has a crucial role during the initial stages of pregnancy [36]. Our findings that the activity of COX-1 represented only a small percentage of total COX activity are in agreement with data reported previously [6]. …”

Section: Discussionsupporting

confidence: 93%

“…Thus, in these cases, LA may need to be obtained from outside sources by consuming certain foods and from supplements [13]. In this study, the fetal weights from V-TD mothers were significantly less than those in both control and in LA-TD groups, which is in agreement with other studies of diabetic embryopathy in vivo [6, 23]. Furthermore, daily injection of LA for 15 days also improved fetal, placental, and membrane mean weight of diabetic rats.…”

Section: Discussionsupporting

confidence: 90%

“…The data from the present study for levels of PGEM and PGFM are not in concordance with those reports. The results from this study and another study [6] showed that, in V-TC fetuses, the levels of PGEM and PGFM were nearly equal while, in the placentas and membranes, the level of PGEM was higher than that of PGFM. The general trend was that the levels of PGEM were lower in both the diabetic groups—mainly in those associated with malformation—and had lower total glutathione contents.…”

Section: Discussionsupporting

confidence: 57%

“…Therefore, it is possible that not all the oxygen-free radicals could be neutralized by LA and that other factors, such as arachidonic acid deficiency and altered prostaglandin metabolism [6, 34], may be involved in the pathogenesis of the diabetes-induced congenital malformations.…”

Section: Discussionmentioning

confidence: 99%

“…There has been an ongoing research that seeks to identify the factors that have been involved in diabetic embryopathy, including increased levels of glucose and ketone bodies [2, 3] and reduced levels of arachidonic acid and prostaglandin E 2 (PGE 2 ) [4, 5]. Furthermore, several studies suggested that disequilibrium of oxidant-antioxidants levels prevalent in diabetic patients is a potential cause of these aberrations [6–11]. Increased formation of reactive oxygen species in association with increased teratogenesis has been demonstrated in rat embryos cultured in vitro in medium containing high levels of glucose [7].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Al-Matubsi

Oriquat

Abu-Samak

et al. 2016

Journal of Diabetes Research

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Background. Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers. Methods. Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1; n = 9; and group 2; n = 7) or pregnant diabetic (group 3; n = 10; and group 4; n = 8) rats were treated daily with either LA (groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15. On day 15 of gestation, the fetuses, placentas, and membranes were dissected, examined morphologically, and then homogenized, to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2 (PGEM) and PGF2 α (PGFM) levels. The level of total glutathione was measured in the maternal liver and plasma and in all fetuses. Results. Supplementation of diabetic rats with LA was found to significantly (p < 0.05) reduce resorption rates in diabetic rats and led to a significant (p < 0.05) increase in liver, plasma, and fetuses total glutathione from LA-TD rats as compared to those from V-TD. Decreased levels of PGEM and elevated levels of PGFM in the fetuses, placentas, and membranes were characteristic of experimental diabetic gestation associated with malformation. The levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p < 0.05) higher than those in malformed fetuses from V-TD rats. Conclusions. LA treatment did not completely prevent the occurrence of malformations. Thus, other factors may be involved in the pathogenesis of the diabetes-induced congenital malformations.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Al-Matubsi

Oriquat

Abu-Samak

et al. 2016

Journal of Diabetes Research

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High glucose leads to redistribution of the proteasomal system

2023

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The impact of high glucose on the cellular redox state, causing both induction of antioxidative systems and also enhanced protein oxidation is discussed for a long time. It is established that elevated glucose levels are disrupting the cellular proteostasis and influencing the proteasomal system. However, it is still unresolved whether this is due to a reaction of the cellular proteasomal system towards the high glucose or whether this is a secondary reaction to inflammatory stimuli. Therefore, we used a dermal fibroblast cell line exposed to high glucose in order to reveal whether a response of the proteasomal system takes place. We investigated the α4 and the inducible iβ5 subunits of the 20S proteasome, as well as the Rpn1‐subunit of the 19S proteasomal regulator complex, measured activity of the 20S, 20S1, and 26S proteasome and detected as well changes in expression as a redistribution into the nucleus. Interestingly, while the activity of the proteasomal forms rather decreased under high glucose treatment; higher expression levels of components of the proteasomal system and higher concentrations of protein‐bound 3‐nitrotyrosine and Nrf2 (nuclear factor [erythroid‐derived 2]‐like 2) were detected. However, no change in the cytosol‐nucleus distribution could be detected for most of the quantified parameters. We concluded that high glucose alone, without additional inflammatory stimuli, provokes a regulatory response on the ubiquitin‐proteasomal system.

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Current literature in diabetes

2010

Diabetes Metabolism Res

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of diabetes/metabolism. Each bibliography is divided into 26 sections: 1 Reviews; 2 General; 3 Genetics; 4 Epidemiology; 5 Immunology; 6 Obesity; 7 Prediction and Prevention; 8 Intervention: a) General; b) Care; c) Drug Therapy; d)Economics; e) Gene therapy; f) Nursing; g) Nutrition; h) Surgery; i) Transplantation; 9 Pathology and Complications: a) General; b) Cardiovascular; c) Eye disease; d) Gestational and fetal; e) Neurological; f) Podiatrical; g) Renal; 10 Endocrinology & Metabolism; 11 Experimental Studies; 12 Diagnosis and Techniques. Within each section, articles are listed in alphabetical order with respect to author

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Activities of cyclooxygenases, and levels of prostaglandins E2 And F2α, in fetopathy associated with experimental diabetic gestation

Cited by 14 publications

References 42 publications

Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

High glucose leads to redistribution of the proteasomal system

Current literature in diabetes

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Activities of cyclooxygenases, and levels of prostaglandins E2 And F2α, in fetopathy associated with experimental diabetic gestation

Cited by 14 publications

References 42 publications

Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2and F2αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2and F2αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

High glucose leads to redistribution of the proteasomal system

Current literature in diabetes

Contact Info

Product

Resources

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Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E₂and F₂αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes