Activities of carboxylation enzymes and products of 14CO2 fixation in photoautotrophically cultured cells

Sato, Fumihiko; Nishida, Kojiro; Yamada, Yoshihiko

doi:10.1016/0304-4211(80)90027-9

Cited by 26 publications

(9 citation statements)

References 17 publications

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“…The reaction was initiated by adding 0.2 mM RuBP, and activity was assayed spectrophotometrically at 340 nm for 10 min at 30°C (Sato et al 1980). Enzyme activities were expressed as lmol h -1 g -1 .…”

Section: Enzyme Extraction and Assaymentioning

confidence: 99%

Responses of Arabidopsis thaliana to bicarbonate-induced iron deficiency

et al. 2009

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Morpho-physiological and biochemical responses of Arabidopsis thaliana (accession N1438) to bicarbonate-induced iron deficiency were investigated. Plants were grown in cabinet under controlled conditions, in a nutrient solution containing 5 lM Fe, added or not with 10 mM NaHCO 3 . After 30 days, bicarbonate-treated plants displayed significantly lower biomass, leaf number and leaf surface area as compared to control plants, and slight yellowing of their younger leaves was observed. Potassium (K ? ) content was not modified by bicarbonate treatment in roots, whereas it was significantly diminished in shoots. Their content in ferrous iron (Fe 2? ) and in leaf total chlorophylls was noticeably lower than in control plants. Root Fe(III)-chelate reductase and phosphoenolpyruvate carboxylase (PEPC) activities were significantly enhanced, but leaf ribulose 1.5-bisphosphate carboxylase (Rubisco) activity was decreased.

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Section: Enzyme Extraction and Assaymentioning

confidence: 99%

Responses of Arabidopsis thaliana to bicarbonate-induced iron deficiency

et al. 2009

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“…Rubisco activity: The activity (Rubisco) (EC 4.1.1.39) was measured in a crude leaf extract following Sato et al (1980). The assay reaction mixture consisted of 50 mM Tris-HCl buffer (pH 8.0), 10 mM NaHCO 3 , 5 mM MgCl 2 , 2 mM NADH, 1 mM ATP, 1 mM DTT, 0.5 mM RuBP, 5 U glyceraldehyde-3-phosphodehydrogenase, and 5 U phosphoglycerate kinase in a final volume of 3 mL.…”

Section: Photosynthetsis In Salvinia Exposed To Chromium and Zinc Ricmentioning

confidence: 99%

Photosynthetic performance of Salvinia natans exposed to chromium and zinc rich wastewater

Dhir

Sharmila

Saradhi

2008

Braz. J. Plant Physiol.

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Investigations were carried out to evaluate alterations in photosynthetic performance of Salvinia natans (L.) exposed to chromium (Cr) and zinc (Zn) rich wastewater. Accumulation of high levels of Cr and Zn in plants affected photosynthetic electron transport. Photosystem-(PS) II-mediated electron transport was enhanced in plants exposed to Cr rich wastewater while a decline was observed in Zn-exposed plants. Photosystem-I-mediated electron transport increased in plants exposed to Cr and Zn rich wastewater. Efficiency of photosystem II (F v /F m ) measured by fluorescence did not show any significant change in Cr-exposed plants but a decrease was observed in Zn-exposed plants as compared to the control. The enhancement in PS I-induced cyclic electron transport in Cr and Zn exposed plants led to a build up of the transthylakoidal proton gradient (∆pH) which subsequently helped in maintaining the photophosphorylation potential to meet the additional requirement of ATP under stress. The carbon assimilation potential was adversely affected as evident from the decrease in Rubisco (EC 4.1.1.39) activity. The alterations in photosynthetic electron transport affected stromal redox status and induced variations in the level of stromal components such as pyridine nucleotides in plants exposed to Cr and Zn rich wastewater. The present investigations revealed that alteration in the photosynthetic efficiency of Salvinia exposed to Cr could primarily be the result of a decline in carbon assimilation efficiency relative to light-mediated photosynthetic electron transport, though in the case of Zn-exposed plants both these factors were affected equally. Key words: carbon assimilation, electron transport, heavy metals, photophosphorylation, redox status Desempenho fotossintético de plantas de Salvinia natans expostas a água de esgoto rica em cromo e zinco: Avaliou-se o desempenho fotossintético de plantas de Salvinia natans L. expostas a água de esgoto rica em cromo (Cr) e zinco (Zn). O acúmulo de Cr e Zn em altos níveis afetou o transporte fotossintético de elétrons. O transporte de elétrons mediado pelo fotossistema (FS) II aumentou nas plantas expostas ao esgoto rico em Cr, observando-se o oposto nas plantas expostas ao esgoto rico em Zn. O transporte de elétrons mediado pelo FS I aumentou nas plantas expostas ao esgoto rico tanto em Cr como em Zn. A eficiência do FS II (F v /F m ) avaliada por fluorescência não variou significativamente em resposta ao Cr, porém reduziu-se nas plantas expostas ao esgoto rico em Zn, em comparação com as plantas-controle. O aumento do transporte cíclico de elétrons associado ao FS I acarretou em aumento do gradiente transtilacoidal de prótons (∆pH) que, por seu turno, esteve associado com o potencial de fotofosforilação para suportar as necessidades adicionais de ATP sob condições de estresse. O potencial de assimilação do carbono foi decrescido, conforme se deduz da menor atividade da Rubisco (EC 4.1.1.39). As alterações no transporte fotossintético de elétrons afetaram o status redox do estro...

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“…This was primarily due to low total Rubisco activity rather than much higher PEP carboxylase activity (3). Photoautotrophic cells of several C3 species have been shown to incorporate more CO2 via PEP carboxylase than cells from leaves of C3 plants (3,5,9,16,23).…”

Section: Pep Carboxylase Activitymentioning

confidence: 99%

Photosynthetic Characterization of Photoautotrophic Cells Cultured in a Minimal Medium

Goldstein¹,

Widholm²

1990

Plant Physiol.

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Photosynthetic properties of photoautotrophic suspensions cultured in a minimal growth medium have been evaluated to determine whether changes have occurred in ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity, phosphoenol-pyruvate (PEP) carboxylase activity, chlorophyll content, or culture growth. Five photoautotrophic lines Amaranthus powellii, Datura innoxia, Glycine max, Gossypium hirsutum, and a Nicotiana tabacum-Nicotiana glutinosa fusion hybrid were grown in a medium without organic carbon other than phytohormones, and without vitamins. These photoautotrophic lines had total Rubisco activities ranging from 85 to 266 micromoles CO2 fixed per milligram chlorophyll hour-1, with percent activation of Rubisco ranging from 16 to 53%. Inclusion of protease inhibitors in the homogenization buffer did not result in higher Rubisco activity. PEP carboxylase activity for cells cultured in minimal medium was found to range from 16 to 146 micromoles CO2 per milligram chlorophyll hour-', with no higher activity in the C4 Amaranthus cells compared with PEP carboxylase activity in the C3 species assayed. Rubisco-to-PEP carboxylase ratios ranged from 2.2 to 1 up to 9.4 to 1. Chlorophyll contents increased in all but the Nicotiana cell line, and all of the photoautotrophic culture lines were capable of growth in vitamin-free medium with the exception of SB-P, which requires thiamine.

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Activities of carboxylation enzymes and products of 14CO2 fixation in photoautotrophically cultured cells

Cited by 26 publications

References 17 publications

Responses of Arabidopsis thaliana to bicarbonate-induced iron deficiency

Responses of Arabidopsis thaliana to bicarbonate-induced iron deficiency

Photosynthetic performance of Salvinia natans exposed to chromium and zinc rich wastewater

Photosynthetic Characterization of Photoautotrophic Cells Cultured in a Minimal Medium

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