Activities of bacteria‐free preparations from <i>Vibrio cholerae</i>

De, S. N.; Ghose, M. L.; Sen, A.

doi:10.1002/path.1700790219

Cited by 37 publications

(10 citation statements)

References 7 publications

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“…The abscissa give the dilution of the PSUP toxin from cultures of different ' classical ' (~34580 and D34580GP) and EL TOR (133,282,929) vibrios. The variations in the quantities of toxin produced by the strains agreed with the findings of other workers (De, Ghose & Sen, 1960;Finkelstein et al 1964;Burrows, 1968).…”

Section: Serological Methodssupporting

confidence: 90%

Studies on Cholera Toxin and Antitoxin

Ungar

Stanić

Chariatte

et al. 1970

Journal of General Microbiology

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Section: Serological Methodssupporting

confidence: 90%

Studies on Cholera Toxin and Antitoxin

Ungar

Stanić

Chariatte

et al. 1970

Journal of General Microbiology

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“…Thus De et al (1960De et al ( , 1962 reported the liberation of cholera exo-toxin in young cultures of vibrios in peptone water medium, and Finkelstein et al (1964) found the presence of choleragenic products, as tested by the infant rabbit technique (Dutta & Habbu, 1955), in young cultures of the vibrios in brain heart infusion broth and also in the syncase medium of Finkelstein ef al. (1966).…”

Section: S N Chatterjee and 3 D A Smentioning

confidence: 94%

“…It has been reported (Gallut, 1954) that the toxins are released when the cholera vibrios are incubated in glucose saline medium at 37" for 4-6 hr. Recent investigations (Burrows, 1965;De, Ghose & Sen, 1960;De, Ghose & Chandra, 1962;Finkelstein, Noms & Dutta, 1964;Finkelstein, 1965) have indicated that the choleragenic products are likely to be liberated by the young vibrios presumably by a secretion process; however conclusive evidence for this has not yet been obtained.…”

Section: Introductionmentioning

confidence: 93%

Electron Microscopic Observations on the Excretion of Cell-wall Material by Vibrio cholerae

Chatterjee¹,

Das²

1967

Journal of General Microbiology

222

134

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S U M M A R YThin sections of Vibrio cholerae harvested during the logarithmic phase of growth in alkaline peptone water or in syncase medium have revealed an excretion process of the cell wall in the form of bulging out and pinching-off of portions. An identical phenomenon has also been revealed in cells harvested after a short period of incubation (1-5 hr) in glucose saline solution at 37". Particles closely resembling the pinched-off cell-wall structures have been detected by metal shadowing and negative staining techniques in the bacteriafree atrates of the log phase cultures (in both media) and the lucose saline a maximum frequency in the range 600-800 A. No similar cell-wall changes have been detected in vibrios harvested from the stationary phase of growth in any of the culture media, nor in vibrios undergoing plasmolysis. Turbidimetric tests revealed no significant lysis of the vibrios when harvested during the logarithmic growth phase and incubated for several hours in saline or in Kellenberger buffer as compared to the lysis detected in distilled water. It is suggested that the cell-wall process described represents an excretory mechanism of V. cholerae, and the nature of the products released by the young vibrios and their probable relation with cholera toxin is discussed. incubation medium. These particles are in the range 400-1 IOO x in size with

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“…Initially ligated loops of rabbit small intestine were used as a model to assess the contribution of putative virulence factors to bacterial pathogen-induced diarrhea. This model was used for Vibrio cholerae, where the injection of whole cultures (24,25), culture supernatants (26), and cell extracts (27) caused dilation of the loop due to fluid accumulation. The rabbit ileal loop model also has been used to study pathogenesis of Escherichia coli (28)(29)(30), Salmonella (20,31,32), Shigella (33), Pseudomonas aeruginosa (34), Clostridium perfringens (35), Vibrio parahaemolyticus, Vibrio alcaligenes (36), and Bacillus cereus (37).…”

mentioning

confidence: 99%

Inflammatory Effects of Edwardsiella ictaluri Lipopolysaccharide Modifications in Catfish Gut

et al. 2014

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e Bacterial lipopolysaccharides (LPS) are structural components of the outer membranes of Gram-negative bacteria and also are potent inducers of inflammation in mammals. Higher vertebrates are extremely sensitive to LPS, but lower vertebrates, like fish, are resistant to their systemic toxic effects. However, the effects of LPS on the fish intestinal mucosa remain unknown. Edwardsiella ictaluri is a primitive member of the Enterobacteriaceae family that causes enteric septicemia in channel catfish (Ictalurus punctatus). E. ictaluri infects and colonizes deep lymphoid tissues upon oral or immersion infection. Both gut and olfactory organs are the primary sites of invasion. At the systemic level, E. ictaluri pathogenesis is relatively well characterized, but our knowledge about E. ictaluri intestinal interaction is limited. Recently, we observed that E. ictaluri oligo-polysaccharide (O-PS) LPS mutants have differential effects on the intestinal epithelia of orally inoculated catfish. Here we evaluate the effects of E. ictaluri O-PS LPS mutants by using a novel catfish intestinal loop model and compare it to the rabbit ileal loop model inoculated with Salmonella enterica serovar Typhimurium LPS. We found evident differences in rabbit ileal loop and catfish ileal loop responses to E. ictaluri and S. Typhimurium LPS. We determined that catfish respond to E. ictaluri LPS but not to S. Typhimurium LPS. We also determined that E. ictaluri inhibits cytokine production and induces disruption of the intestinal fish epithelia in an O-PS-dependent fashion. The E. ictaluri wild type and ⌬wibT LPS mutant caused intestinal tissue damage and inhibited proinflammatory cytokine synthesis, in contrast to E. ictaluri ⌬gne and ⌬ugd LPS mutants. We concluded that the E. ictaluri O-PS subunits play a major role during pathogenesis, since they influence the recognition of the LPS by the intestinal mucosal immune system of the catfish. The LPS structure of E. ictaluri mutants is needed to understand the mechanism of interaction.

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Activities of bacteria‐free preparations from Vibrio cholerae

Cited by 37 publications

References 7 publications

Studies on Cholera Toxin and Antitoxin

Studies on Cholera Toxin and Antitoxin

Electron Microscopic Observations on the Excretion of Cell-wall Material by Vibrio cholerae

Inflammatory Effects of Edwardsiella ictaluri Lipopolysaccharide Modifications in Catfish Gut

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